Inducing Experimental Polymicrobial Sepsis by Cecal Ligation and Puncture [0.03%]
通过结肠结扎穿孔诱导实验性多微生物脓毒症
Frances V Sjaastad,Isaac J Jensen,Roger R Berton et al.
Frances V Sjaastad et al.
Numerous models are available for the preclinical study of sepsis, and they fall into one of three general categories: (1) administration of exogenous toxins (e.g., lipopolysaccharide, zymosan), (2) virulent bacterial or viral challenge, an...
Haitao Guo,Jenny P-Y Ting
Haitao Guo
This article presents assays that allow induction and measurement of activation of different inflammasomes in mouse macrophages, human peripheral blood mononuclear cell (PBMC) cultures, and mouse peritonitis and endotoxic shock models. Basi...
Purification, Culture, and CD19-CAR Lentiviral Transduction of Adult and Umbilical Cord Blood NK Cells [0.03%]
成人和脐带血NK细胞的纯化、培养以及CD19-CAR慢病毒转导
Lara Herrera,Manel Juan,Cristina Eguizabal
Lara Herrera
Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. The role that NK cells play is analogous to that of cytotoxic T cells in that they provide rapid responses to virus-infected cells a...
Analysis of Antigen-Specific Human Memory B Cell Populations Based on In Vitro Polyclonal Stimulation [0.03%]
基于体外多克隆刺激的抗原特异性人记忆B细胞群分析
Phuong Nguyen-Contant,A Karim Embong,David J Topham et al.
Phuong Nguyen-Contant et al.
Antigen-specific memory B cell (MBC) populations mediate the rapid, strong, and high-affinity secondary antibody responses that play a key role in combating infection and generating protective responses to vaccination. Recently, cell staini...
Tanushree Dangi,Young Rock Chung,Nicole Palacio et al.
Tanushree Dangi et al.
In this invited article, we explain technical aspects of the lymphocytic choriomeningitis virus (LCMV) system, providing an update of a prior contribution by Matthias von Herrath and J. Lindsay Whitton. We provide an explanation of the LCMV...
Establishment and Culture of Human Intestinal Organoids Derived from Adult Stem Cells [0.03%]
来成年人干细胞的人类肠道类器官的建立和培养
Cayetano Pleguezuelos-Manzano,Jens Puschhof,Stieneke van den Brink et al.
Cayetano Pleguezuelos-Manzano et al.
Human intestinal organoids derived from adult stem cells are miniature ex vivo versions of the human intestinal epithelium. Intestinal organoids are useful tools for the study of intestinal physiology as well as many disease conditions. The...
Deepali Rathore,Aleksandra Nita-Lazar
Deepali Rathore
Immune cell signaling is largely regulated by protein phosphorylation. Stimulation of toll-like receptors (TLRs) by pathogen-associated ligands drives the cascade of immune response, which can be influenced by differences in phosphoprotein ...
Immunopharmacology and Quantitative Analysis of Tyrosine Kinase Signaling [0.03%]
酪氨酸激酶信号的免疫药理学和定量分析
Ben F Brian th,Candace R Guerrero,Tanya S Freedman
Ben F Brian th
In this article we describe the use of pharmacological and genetic tools coupled with immunoblotting (Western blotting) and targeted mass spectrometry to quantify immune signaling and cell activation mediated by tyrosine kinases. Transfer o...
Anna S Tocheva,Shalom Lerrer,Adam Mor
Anna S Tocheva
Our understanding of programmed cell death 1 (PD-1) biology is limited due to technical difficulties in establishing reproducible, yet simple, in vitro assays to study PD-1 signaling in primary human T cells. The protocols in this article w...
A Novel In Vitro Mouse Model to Study Mycobacterium tuberculosis Dissemination Across Brain Vessels: A Combination Granuloma and Blood-Brain Barrier Mouse Model [0.03%]
一种新的小鼠体外模型研究结核分枝杆菌通过脑血管扩散：组合肉芽肿和血脑屏障的小鼠模型
Fruzsina R Walter,Trey E Gilpin,Melinda Herbath et al.
Fruzsina R Walter et al.
In vitro culture models of the blood-brain barrier (BBB) provide a useful platform to test the mechanisms of cellular infiltration and pathogen dissemination into the central nervous system (CNS). We present an in vitro mouse model of the B...