Characterization and Functional Analysis of Mouse Semi-invariant Natural T Cells [0.03%]
小鼠半不变自然T细胞的特征和功能分析
Amrendra Kumar,Jelena S Bezbradica,Aleksandar K Stanic et al.
Amrendra Kumar et al.
Semi-invariant natural killer T (iNKT) cells are CD1d-restricted innate-like lymphocytes that recognize lipid agonists. Activated iNKT cells have immunoregulatory properties. Human and mouse iNKT cell functions elicited by different glycoli...
M Michele Hogan,Stefanie N Vogel
M Michele Hogan
The tumor necrosis factor (TNF) superfamily of cytokines plays critical roles in all aspects of the immune response. TNF and the lymphotoxins (LT), LTα and LTβ, are particularly important as major effector cytokines and mediators or lymph...
Transfection by Electroporation [0.03%]
电穿孔转染
Huntington Potter,Richard Heller
Huntington Potter
Electroporation-the use of high-voltage electric shocks to introduce DNA into cells-can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps,...
Marie-Paule Lefranc
Marie-Paule Lefranc
This appendix includes a selection of links of interest to immunologists from the international ImMunoGeneTics (IMGT) database, covering a wide variety of subject matter. © 2017 by John Wiley & Sons, Inc. ...
Double-Immunodiffusion Assay for Detecting Specific Antibodies (Ouchterlony) [0.03%]
双向免疫扩散试验(Ouchterlony双扩散实验)
Peter Hornbeck
Peter Hornbeck
The method first described by Ouchterlony in 1948 is a classic and simple technique that permits evaluation and comparison of antibodies in animal or human sera directed against protein or complex carbohydrate antigens. It is a low-tech pro...
Peter Hornbeck,Thomas A Fleisher,Nicholas M Papadopoulos
Peter Hornbeck
On identifying a new monoclonal antibody, or in characterizing antibodies in sera evoked by disease or immunization, it is particularly informative to determine the serological class of the antibodies. The serological class of the protein i...
Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy [0.03%]
紫外吸收及荧光光谱法测定DNA和RNA的浓度
Sean R Gallagher
Sean R Gallagher
Quantitation of nucleic acids is a fundamental tool in molecular biology that requires accuracy, reliability, and the use of increasingly smaller sample volumes. This unit describes the traditional absorbance measurement at 260 nm and three...
Transduction of Herpesvirus saimiri-Transformed T Cells with Exogenous Genes of Interest [0.03%]
携带有意义的外源基因的猿猴逆转录病毒(Simian sarcoma virus)转化T细胞的转导
Rubén Martínez-Barricarte,Sarah Jill de Jong,Janet Markle et al.
Rubén Martínez-Barricarte et al.
Human T cells can be transformed and expanded with herpesvirus saimiri (HVS). HVS-transformed T cells from patients have facilitated the study of a broad range of primary immunodeficiencies (PID) in which T-cell development or function is a...
Carmelo Carmona-Rivera,Mariana J Kaplan
Carmelo Carmona-Rivera
Neutrophil extracellular traps (NET) are web-like structures composed of nuclear material and neutrophil granular proteins that are released to the extracellular space after specific microbial or sterile inflammatory stimuli. NETosis is a s...
Isolation and Characterization of Exosomes from Cultures of Tissue Explants and Cell Lines [0.03%]
组织切片和细胞系培养物中分离及表征外泌体
Lucia Mincheva-Nilsson,Vladimir Baranov,Olga Nagaeva et al.
Lucia Mincheva-Nilsson et al.
Exosomes are specialized, nanometer-sized extracellular vesicles of endosomal origin actively secreted into the extracellular space by a variety of cells under normal and pathological conditions. Exosomes have recently emerged as important ...