Next-Generation Sequencing Library Preparation from FFPE Tissue Samples [0.03%]
福尔马林固定石蜡包埋组织样本的下一代测序文库构建方法
Kathrin Wolf,Dominic O&#x;Neil,Stefanie Schroeer et al.
Kathrin Wolf et al.
Formalin-fixed, paraffin-embedded (FFPE) tissue samples represent an invaluable biobank for retrospective cancer research with molecular methods such as real-time PCR and next-generation sequencing (NGS). However, the usage of FFPE material...
Marian F Laughery,John J Wyrick
Marian F Laughery
CRISPR-Cas9 has emerged as a powerful method for editing the genome in a wide variety of species, since it can generate a specific DNA break when targeted by the Cas9-bound guide RNA. In yeast, Cas9-targeted DNA breaks are used to promote h...
CRISPR-Cas9-Guided Genome Engineering in Caenorhabditis elegans [0.03%]
基于CRISPR-Cas9的基因组编辑在秀丽隐杆线虫中的应用
Hyun-Min Kim,Monica P Colaiácovo
Hyun-Min Kim
The CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein) system is being used successfully for efficient and targeted genome editing in various organisms, including the nematode Caenorhabditis ele...
deltaTE: Detection of Translationally Regulated Genes by Integrative Analysis of Ribo-seq and RNA-seq Data [0.03%]
通过整合Ribo-seq和RNA-seq数据检测翻译调控基因的方法(deltaTE)
Sonia Chothani,Eleonora Adami,John F Ouyang et al.
Sonia Chothani et al.
Ribosome profiling quantifies the genome-wide ribosome occupancy of transcripts. With the integration of matched RNA sequencing data, the translation efficiency (TE) of genes can be calculated to reveal translational regulation. This layer ...
Optimizing Tissue Preservation for High-Resolution Confocal Imaging of Single-Molecule RNA-FISH [0.03%]
用于单分子RNA-FISH的高分辨率共聚焦成像的组织保存优化方法
Nash Redmayne,Shawn L Chavez
Nash Redmayne
Over the past century, formalin-fixed, paraffin-embedded (FFPE) tissue samples have represented the standard for basic histology and immunostaining. However, FFPE has several limitations and less stringent tissue preservation methods are re...
RNA Fragmentation and Sequencing (RF-Seq): Cost-Effective, Time-Efficient, and High-Throughput 3' mRNA Sequencing Library Construction in a Single Tube [0.03%]
RNA片段化与测序（RF-seq）：单管构建高效、快速且通量高的3' mRNA 测序文库的方法
Yaligara Veeranagouda,Anne Remaury,Jean-Claude Guillemot et al.
Yaligara Veeranagouda et al.
Over the past decade, transcriptomic studies using next-generation sequencing (NGS)-based RNA sequencing (RNA-Seq) have greatly contributed to characterizing biochemical and physiological changes in cells and tissues across organisms and ex...
Raghuvir Viswanatha,Roderick Brathwaite,Yanhui Hu et al.
Raghuvir Viswanatha et al.
High-throughput screens in Drosophila melanogaster cell lines have led to discovery of conserved gene functions related to signal transduction, host-pathogen interactions, ion transport, and more. CRISPR/Cas9 technology has opened the door ...
Rapid Tagging of Human Proteins with Fluorescent Reporters by Genome Engineering using Double-Stranded DNA Donors [0.03%]
利用双链DNA供体进行基因组工程快速对人类蛋白质进行荧光标记技术
Alexandre Paix,Dominique Rasoloson,Andrew Folkmann et al.
Alexandre Paix et al.
Tagging proteins with fluorescent reporters such as green fluorescent protein (GFP) is a powerful method to determine protein localization, especially when proteins are tagged in the endogenous context to preserve native genomic regulation....
Rajaraman Gopalakrishnan,Fred Winston
Rajaraman Gopalakrishnan
The budding yeast, Saccharomyces cerevisiae, has been widely used for genetic studies of fundamental cellular functions. The isolation and analysis of yeast mutants is a commonly used and powerful technique to identify the genes that are in...
Auxin-Inducible Degron System for Depletion of Proteins in Saccharomyces cerevisiae [0.03%]
应用于酿酒酵母的吲哚乙酸诱导降解系统的蛋白质耗竭方法
Ameet Shetty,Natalia I Reim,Fred Winston
Ameet Shetty
The auxin-inducible degron (AID) is a powerful tool that is used for depletion of proteins to study their function in vivo. This method can conditionally induce the degradation of any protein by the proteasome simply by the addition of the ...