Base Editing in Human Cells to Produce Single-Nucleotide-Variant Clonal Cell Lines [0.03%]
在人类细胞中进行碱基编辑以产生单核苷酸变异克隆细胞系
Carlos A Vasquez,Quinn T Cowan,Alexis C Komor
Carlos A Vasquez
Base-editing technologies enable the introduction of point mutations at targeted genomic sites in mammalian cells, with higher efficiency and precision than traditional genome-editing methods that use DNA double-strand breaks, such as zinc ...
Kirsten A Reimer,Karla M Neugebauer
Kirsten A Reimer
Long read sequencing technologies now allow high-quality sequencing of RNAs (or their cDNAs) that are hundreds to thousands of nucleotides long. Long read sequences of nascent RNA provide single-nucleotide-resolution information about co-tr...
Katherine P Gill,Mark Denham
Katherine P Gill
The lentivirus system enables efficient genetic modification of both dividing and non-dividing cells and therefore is a useful tool for elucidating developmental processes and disease pathogenesis. The development of third-generation lentiv...
Virion Display: A High-Throughput Method to Express Functional Membrane Proteins [0.03%]
病毒粒子展示：表达功能膜蛋白的高通量方法
Guan-Da Syu,Eric Johansen,Heng Zhu
Guan-Da Syu
Transmembrane proteins are responsible for many critical cellular functions and represent one of the largest families of drug targets. However, these proteins, especially multipass transmembrane proteins, are difficult to study because they...
ARF-AID: A Rapidly Inducible Protein Degradation System That Preserves Basal Endogenous Protein Levels [0.03%]
ARF-AID：一种可快速诱导的蛋白质降解系统，保留基础内源性蛋白水平
Kizhakke Mattada Sathyan,Thomas G Scott,Michael J Guertin
Kizhakke Mattada Sathyan
Inducible degron systems are widely used to specifically and rapidly deplete proteins of interest in cell lines and organisms. An advantage of inducible degradation is that the biological system under study remains intact and functional unt...
High-Throughput Cellular RNA Sequencing (HiCAR-Seq): Cost-Effective, High-Throughput 3' mRNA-Seq Method Enabling Individual Sample Quality Control [0.03%]
高通量细胞RNA测序（HiCAR-Seq）：一种经济高效的高通量3'mRNA测序方法，实现单个样本质量控制
Yaligara Veeranagouda,Jean-Luc Zachayus,Jean-Claude Guillemot et al.
Yaligara Veeranagouda et al.
High-throughput screening is one of the pillars of drug development. Unbiased transcriptome profiling is now widely used for a deeper understanding of a drug's mechanisms of action, off target effects, and cytotoxicity. Although currently a...
Simultaneous Examination of Cellular Pathways using Multiplex Hextuple Luciferase Assaying [0.03%]
利用六联萤火虫荧光素酶检测系统同时分析细胞信号通路
Alejandro Sarrion-Perdigones,Lyra Chang,Yezabel Gonzalez et al.
Alejandro Sarrion-Perdigones et al.
Multiplex experimentation that can assay multiple cellular signaling pathways in the same cells requires orthogonal genetically encoded reporters that report over large dynamic ranges. Luciferases are cost-effective, versatile candidates wh...
Rapid and Efficient Synthetic Assembly of Multiplex Luciferase Reporter Plasmids for the Simultaneous Monitoring of Up to Six Cellular Signaling Pathways [0.03%]
一种快速高效地用于同时监测多达六条细胞信号转导通路的多重萤火虫荧光素酶报告质粒合成型组装方法
Alejandro Sarrion-Perdigones,Yezabel Gonzalez,Koen J T Venken
Alejandro Sarrion-Perdigones
High-throughput cell-based screening assays are valuable tools in the discovery of chemical probes and therapeutic agents. Such assays are designed to examine the effects of small compounds on targets, pathways, or phenotypes participating ...
Proximity-CLIP and Expedited Non-Radioactive Library Preparation of Small RNA Footprints for Next-Generation Sequencing [0.03%]
邻近连接的CLIP和小RNA印记的快速非放射性文库制备用于下一代测序技术
Dimitrios Anastasakis,Daniel Benhalevy,Markus Hafner
Dimitrios Anastasakis
During the course of their life cycle, most RNAs move between several cellular environments where they associate with different RNA binding proteins (RBPs). Reciprocally, a significant portion of RBPs reside in more than a single cellular c...
Shai Rosenstein,Anna Vaisman-Mentesh,Limor Levy et al.
Shai Rosenstein et al.
Antibodies are widely used in therapeutic, diagnostic, and research applications, and antibody derivatives such as F(ab')2 fragments are used when only a particular antibody region is required. F(ab')2 can be produced through antibody engin...