Epimerization and Decomposition of Kojibiose and Sophorose by Heat Treatment under Neutral pH Conditions [0.03%]
中性条件下加热导致 kojibiose 和 sophorose 的差向异构化和降解
Kazuhiro Chiku,Mami Wada,Haruka Atsuji et al.
Kazuhiro Chiku et al.
We evaluated the stabilities of kojibiose and sophorose when heated under neutral pH conditions. Kojibiose and sophorose epimerized at the C-2 position of glucose on the reducing end, resulting in the production of 2-O-α-D-glucopyranosyl-D...
Cellulase Production of Trichoderma reesei (Hypocrea jecorina) by Continuously Fed Cultivation Using Sucrose as Primary Carbon Source [0.03%]
以蔗糖为主要碳源的里氏木霉（球抱白僵菌）纤维素酶流加培养产酶研究
Masakazu Ike,Ken Tokuyasu
Masakazu Ike
To expand the range of soluble carbon sources for our enzyme production system, we investigated the properties of sucrose utilization and its effect on cellulase production by Trichoderma reesei M2-1. We performed batch cultivation of T. re...
Preparation of a Molecular Library of Branched β-Glucan Oligosaccharides Derived from Laminarin [0.03%]
以海藻糖为原料的分支β-葡聚糖寡糖分子库的构建
Shunji Natsuka,Aki Tachibana,Wataru Sumiyoshi et al.
Shunji Natsuka et al.
To study the structure of β-glucans, we developed a separation method and molecular library of β-glucan oligosaccharides. The oligosaccharides were prepared by partial acid hydrolysis from laminarin, which is a β-glucan of Laminaria digi...
Glucosamine Extends the Lifespan of Caenorhabditis elegans via Autophagy Induction [0.03%]
葡萄糖胺通过诱导自噬延长线虫寿命
Tomoya Shintani,Yuhei Kosuge,Hisashi Ashida
Tomoya Shintani
Glucosamine (GlcN) is commonly used as a dietary supplement to promote cartilage health in humans. We previously reported that GlcN could induce autophagy in cultured mammalian cells. Autophagy is known to be involved in the prevention of v...
Molecular Design and Synthesis of a Novel Substrate for Assaying Lysozyme Activity [0.03%]
LYZ新型底物的设计、合成及应用研究
Megumi Matsui,Haruka Kono,Makoto Ogata
Megumi Matsui
A novel substrate {Galβ1,4GlcNAcβ1,4GlcNAc-β-pNP [Gal(GlcNAc)2-β-pNP]} for assaying lysozyme activity has been designed using docking simulations and enzymatic synthesis via β-1,4-galactosyltransferase-mediated transglycosylation from ...
Characterization of a GH36 β-L-Arabinopyranosidase in Bifidobacterium adolescentis [0.03%]
青春双歧杆菌GH36型β-L-阿拉伯糖吡喃糖苷酶的特性分析
Yuki Sasaki,Nami Togo,Kanefumi Kitahara et al.
Yuki Sasaki et al.
β-L-Arabinopyranosidases are classified into the glycoside hydrolase family 27 (GH27) and GH97, but not into GH36. In this study, we first characterized the GH36 β-L-arabinopyranosidase BAD_1528 from Bifidobacterium adolescentis JCM1275. ...
Purification, Cloning, Functional Expression, Structure, and Characterization of a Thermostable β-Mannanase from Talaromyces trachyspermus B168 and Its Efficiency in Production of Mannooligosaccharides from Coffee Wastes [0.03%]
来源于Talaromyces trachyspermus B168的耐热性β-甘露聚糖酶的纯化、克隆、功能表达、结构及其在生产咖啡废弃物中甘露低聚糖中的效率
Kentaro Suzuki,Mari Michikawa,Haruna Sato et al.
Kentaro Suzuki et al.
Highly thermostable β-mannanase, belonging to glycoside hydrolase family 5 subfamily 7, was purified from the culture supernatant of Talaromyces trachyspermus B168 and the cDNA of its transcript was cloned. The recombinant enzyme showed ma...
Identification of a Point Mutation in the Granule-bound Starch Synthase I Gene (GBSSI) in a waxy Diploid Wheat Mutant and Design of Molecular Markers for Backcrossing [0.03%]
二倍体蜡质小麦突变体淀粉合成酶I基因（GBSSI）中点突变的鉴定及轮回选择分子标记的设计
Satoko Miura,Naoko Crofts,Misato Abe et al.
Satoko Miura et al.
In cereals, granule-bound starch synthase I (GBSSI)-deficient mutants accumulate glutinous (amylose-free) starch in their storage tissues. The amylose-free starch produced by waxy (wx) mutants of hexaploid bread wheat (Triticum aestivum L.)...
Enhanced Azidolysis by the Formation of Stable Ser-His Catalytic Dyad in a Glycoside Hydrolase Family 10 Xylanase Mutant [0.03%]
甘露聚糖内切酶突变体中稳定Ser-His催化二联体的形成及其对叠氮水解反应的促进作用
Ryuichiro Suzuki,Zui Fujimoto,Satoshi Kaneko et al.
Ryuichiro Suzuki et al.
Glycoside hydrolases require carboxyl groups as catalysts for their activity. A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10 possesses Glu128 and Glu236 that respectively function as a...
Structural Analysis of a Novel Oligosaccharide Isolated from Fermented Beverage of Plant Extracts [0.03%]
一种植物提取物发酵饮料寡糖的结构分析
Akira Yamamori,Yusuke Takata,Eri Fukushi et al.
Akira Yamamori et al.
A fermented beverage of plant extracts (Super Ohtaka®) was prepared from about 50 kinds of fruits and vegetables. This natural fermentation was performed by yeast (Zygosaccharomyces spp. and Pichia spp.) and lactic acid bacteria (Leuconost...