Characterization of a GH Family 20 Exo-β- N-acetylhexosaminidase with Antifungal Activity from Streptomyces avermitilis [0.03%]
来源于链霉菌的具有抗真菌活性的家族20氨基己糖苷酶的特性研究
Naoki Shirasaka,Koichi Harazono,Ryota Nakahigashi et al.
Naoki Shirasaka et al.
We characterized SaHEX, which is a glycoside hydrolase (GH) family 20 exo-β-N-acetylhexosaminidase found in Streptomyces avermitilis. SaHEX exolytically hydrolyzed chitin oligosaccharides from their non-reducing ends, and yielded N-acetylg...
A Novel α-Glucosidase of the Glycoside Hydrolase Family 31 from Aspergillus sojae [0.03%]
来源于Aspergillus sojae的甘露聚糖酶家族31成员的新α-葡萄糖苷酶基因的克隆、表达及鉴定
Atsushi Kawano,Yuji Matsumoto,Nozomi Nikaido et al.
Atsushi Kawano et al.
We characterized an α-glucosidase belonging to the glycoside hydrolase family 31 from Aspergillus sojae. The α-glucosidase gene was cloned using the whole genome sequence of A. sojae, and the recombinant enzyme was expressed in Aspergillu...
Construction of Cellulose Binding Domain Fusion FMN-Dependent NADH-Azoreductase and Glucose 1-Dehydrogenase for the Development of Flow Injection Analysis with Fusion Enzymes Immobilized on Cellulose [0.03%]
用于固定化融合酶的流动注射分析的基于细胞结合域的FMN依赖型烟酰胺腺嘌呤二核苷酸还原酶和葡萄糖脱氢酶的构建
Shigekazu Yano,Yukari Hori,Tatsuro Kijima et al.
Shigekazu Yano et al.
The cellulose binding domain (CBD) of cellulosome-integrating protein A from Clostridium thermocellum NBRC 103400 was genetically fused to FMN-dependent NADH-azoreductase (AZR) and glucose 1-dehydrogenase (GDH) from Bacillus subtilis. The f...
Generation of Trichoderma reesei Mutant with Enhanced Xylanase Activity by Using Disparity Mutagenesis [0.03%]
利用差异诱变技术获得具有更强木聚糖酶活性的热带青霉变异株
Taisuke Watanabe,Masashi Nasukawa,Yuki Yoshida et al.
Taisuke Watanabe et al.
In the current study, we attempted to enhance the xylanase activity of Trichoderma reesei ATCC66589 by using disparity mutagenesis, wherein a plasmid harboring proofreading-impaired DNA polymerase δ was inserted. Following selection on xyl...
Physicochemical Properties of Starches from Lotus Rhizomes Harvested in Different Months [0.03%]
不同月份采收的莲根淀粉理化性质分析
Yuji Honda,Tetsuya Yamazaki,Naoya Katsumi et al.
Yuji Honda et al.
We investigated the physicochemical properties of starches extracted from 8 lotus (Nelumbo nucifera Gaertn.) rhizomes harvested in different months (September 2012 to May 2013). The physicochemical properties of the lotus starches depended ...
Sugar Composition in Asparagus Spears and Its Relationship to Soil Chemical Properties [0.03%]
芦笋中糖类的构成及其与土壤化学特性之间的关系
Hideyuki Takahashi,Chiharu Yoshida,Takumi Takeda
Hideyuki Takahashi
Glycoside hydrolases require carboxyl groups as catalysts for their activity. A retaining xylanase from Streptomyces olivaceoviridis E-86 belonging to glycoside hydrolase family 10 possesses Glu128 and Glu236 that respectively function as a...
Three Major Nucleotide Polymorphisms in the Waxy Gene Correlated with the Amounts of Extra-long Chains of Amylopectin in Rice Cultivars with S or L-type Amylopectin [0.03%]
与水稻S型和L型稲米Amylopectin长链含量相关的三个蜡基因核苷酸多态性
Naoko Crofts,Ayaka Itoh,Misato Abe et al.
Naoko Crofts et al.
Extra-long chains (ELC) of amylopectin in rice endosperm are synthesized by granule-bound starch synthase I encoded by the Waxy (Wx) gene, which primarily synthesizes amylose. Previous studies showed that single nucleotide polymorphisms (SN...
Functional Characterization of the GH10 and GH11 Xylanases from Streptomyces olivaceoviridis E-86 Provide Insights into the Advantage of GH11 Xylanase in Catalyzing Biomass Degradation [0.03%]
橄榄绿链霉菌E-86的家族10和家族11木聚糖酶的功能表征为家族11木聚糖酶催化生物质降解提供了新见解
Haruka Yagi,Ryo Takehara,Aika Tamaki et al.
Haruka Yagi et al.
We functionally characterized the GH10 xylanase (SoXyn10A) and the GH11 xylanase (SoXyn11B) derived from the actinomycete Streptomyces olivaceoviridis E-86. Each enzyme exhibited differences in the produced reducing power upon degradation o...
Reusable Floating Beads with Immobilized Xylose-Fermenting Yeast Cells for Simultaneous Saccharification and Fermentation of Lime-Pretreated Rice Straw [0.03%]
固定化木糖发酵酵母细胞的可重复使用浮珠用于石灰预处理稻草同步糖化与发酵
Di Guan,Rui Zhao,Yuan Li et al.
Di Guan et al.
Novel bioreactor beads for simultaneous saccharification and fermentation (SSF) of lime-pretreated rice straw (RS) into ethanol were prepared. Genetically modified Saccharomyces cerevisiae cells expressing genes encoding xylose reductase, x...
Washing Lime-Pretreated Rice Straw with Carbonated Water Facilitates Calcium Removal and Sugar Recovery in Subsequent Enzymatic Saccharification [0.03%]
碳酸水洗涤可促进石灰预处理水稻秸秆糖化过程中钙的去除和糖类物质的回收
Kenji Yamagishi,Masakazu Ike,Di Guan et al.
Kenji Yamagishi et al.
Generally, Ca(OH)2 pretreatment of lignocellulosics for fermentable sugar recovery requires a subsequent washing step for calcium removal and pH control for optimized saccharification. However, washing Ca(OH)2-pretreated feedstock with wate...