Biallelic genome engineering to create isogenic induced pluripotent stem cells modelling Huntington's disease [0.03%]
双等位基因组工程化以生成模拟亨廷顿病的同基因诱导多能干细胞
Hikaru Kurasawa,Yuta Matsuura,Riho Yamane et al.
Hikaru Kurasawa et al.
We developed Huntington's disease (HD) modelling induced pluripotent stem cells (iPSCs) by genome engineering of iPSCs from healthy donors. For this, we established a homologous-recombination-based biallelic substitution technique called th...
Development and application of a sex-linked marker for Herpetospermum pedunculosum based on whole-genome resequencing [0.03%]
基于全基因组重测序的Herpetospermum pedunculosum性连锁标记的开发及应用
An-Ning Li,Zhi-Li Zhou,Xi-Long Wang et al.
An-Ning Li et al.
Sex-specific DNA markers are effective tools for sex identification and sex-controlled breeding of dioecious organisms. The seeds of the dioecious Herpetospermum pedunculosum are utilized in traditional Chinese medicine, and the development...
Tomoaki Watanabe,Shohei Nakata,Tokumasa Horiike
Tomoaki Watanabe
In phylogenetic analysis, long-branch attraction (LBA) occurs when two distantly related species with longer branches are mistakenly grouped as the most closely related species. Previous research addressing this issue has focused on phyloge...
Semi-dominant mutations in the gene encoding histidine kinase influences rice morphology [0.03%]
组氨酸激酶基因半显性突变影响水稻株型
Kaito Chiba,Takumi Tezuka,Mayo Watanabe et al.
Kaito Chiba et al.
Cytokinin plays a major role in the regulation of plant development. It is perceived by receptors with histidine kinase activity to regulate the expression of various transcription factors. In the previous study, we reported a semi-dominant...
Asynchronous evolution of centromeric sequences across chromosomes in Pyricularia oryzae [0.03%]
尖孢镰刀菌不同染色体着丝粒序列的异步进化
Atsumi Morimoto,Thach An Dang,Ken-Ichi Ikeda et al.
Atsumi Morimoto et al.
Centromeres are essential for chromosome segregation, yet they are among the most rapidly evolving regions of the genome. The mechanisms driving this rapid evolution of centromeric sequences are still not well understood. In this study, we ...
Development of a TaqMan-based dosage analysis PCR assay for the molecular diagnosis of 22q11.2 deletion syndrome [0.03%]
一种基于TaqMan的剂量分析PCR试验的方法学研究及在22q11.2缺失综合征分子诊断中的应用研究
Dinali M Ranaweera,Deepthi C de Silva,Duminda Samarasinghe et al.
Dinali M Ranaweera et al.
A 1.5 to 3 Mb microdeletion of chromosome 22q11.2 with loss of multiple genes including histone cell cycle regulator (HIRA) causes 22q11.2 deletion syndrome (22q11.2 DS), a common disorder with variable manifestations including congenital m...
Yu Nakabayashi,Masayuki Seki
Yu Nakabayashi
Nucleosomes are complexes of DNA and histone proteins that form the basis of eukaryotic chromatin. Eukaryotic histones are descended from Archaean homologs; however, how this occurred remains unclear. Our previous genetic analysis on the bu...
Mutations in the 5' untranslated region fine-tune the translational control of heterologously expressed genes [0.03%]
5'-UTR中的突变可精炼异源表达基因的转录调控
Riku Kuse,Kojiro Ishii
Riku Kuse
Strict control of the expression levels of heterologously introduced protein-coding genes is important for the functional analysis of the protein of interest and its effective use in new situations. For this purpose, various promoters with ...
The active ingredient β-sitosterol in Ganoderma regulates CHRM2-mediated aerobic glycolysis to induce apoptosis of lung adenocarcinoma [0.03%]
灵芝中活性成分β-谷甾醇通过调节CHRM2介导的肺腺癌细胞Warburg效应诱导凋亡作用研究
Qiong Zhao,Yuting Pan,Danjia Zhang et al.
Qiong Zhao et al.
Background: β-sitosterol is a natural plant steroidal compound with anti-cancer properties against various tumors. This work attempts to explore the inhibitory effect of β-sitosterol on the progression of lung adenocarc...