Performance of a time-resolved IR facility for assessment of protonation states and polarity changes in carboxyl groups in a large membrane protein, mammalian cytochrome c oxidase, under turnover conditions in a sub-millisecond time resolution [0.03%]
一种时间分辨红外设施的性能评估：用于在亚毫秒级时间分辨率下评估大型膜蛋白——哺乳动物细胞色素c氧化酶的转位条件下羧基质子化态和极性变化的状态
Chen Li,Tatsuhito Nishiguchi,Kyoko Shinzawa-Itoh et al.
Chen Li et al.
Time-resolved IR analyses for the protonation and polarity changes of carboxyl groups involved in proton pump enzymes under turnover conditions are indispensable for elucidation of their proton-pump mechanisms. We have developed a new time-...
In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems [0.03%]
豌豆叶柄皮刺中发生了功能性的光合电子传递尽管LHCII与光系统的动态结合力减弱
Martina Giovanardi,Laura Pantaleoni,Lorenzo Ferroni et al.
Martina Giovanardi et al.
The flexible association of the light harvesting complex II (LHCII) to photosystem (PS) I and PSII to balance their excitation is a major short-term acclimation process of the thylakoid membrane, together with the thermal dissipation of exc...
Online in vivo monitoring of cytosolic NAD redox dynamics in Ustilago maydis [0.03%]
在线检测玉米黑穗病菌细胞内NAD还原型和氧化型比率动态变化
Sandra K Hartmann,Yvonne Stockdreher,Georg Wandrey et al.
Sandra K Hartmann et al.
Maintenance of metabolic redox homeostasis is essential to all life and is a key factor in many biotechnological processes. Changes in the redox state of NAD affect metabolic fluxes, mediate regulation and signal transduction, and thus dete...
Functional roles of tyrosine 185 during the bacteriorhodopsin photocycle as revealed by in situ spectroscopic studies [0.03%]
利用原位光谱研究在视紫红质光循环中酪氨酸185的功能作用
Xiaoyan Ding,Chao Sun,Haolin Cui et al.
Xiaoyan Ding et al.
Tyrosine 185 (Y185), one of the aromatic residues within the retinal (Ret) chromophore binding pocket in helix F of bacteriorhodopsin (bR), is highly conserved among the microbial rhodopsin family proteins. Many studies have investigated th...
Network analysis of a proposed exit pathway for protons to the P-side of cytochrome c oxidase [0.03%]
线粒体呼吸链细胞色素c氧化酶P侧质子出口通道的网络分析研究
Xiuhong Cai,Kamran Haider,Jianxun Lu et al.
Xiuhong Cai et al.
Cytochrome c Oxidase (CcO) reduces O2, the terminal electron acceptor, to water in the aerobic, respiratory electron transport chain. The energy released by O2 reductions is stored by removing eight protons from the high pH, N-side, of the ...
ADP-inhibited structure of non-catalytic site-depleted FoF1-ATPase from thermophilic Bacillus sp. PS-3 [0.03%]
来自嗜热芽孢杆菌PS-3的非催化位点贫乏FoF1 ATP酶的ADP抑制结构
The F1 domain of FoF1-ATP synthases/ATPases (FoF1) possesses three catalytic sites on the three αβ interfaces, termed αEβE, αDβD, and αTβT, located mainly on the β subunits. The enzyme also has three non-catalytic ATP-binding sites...