High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation [0.03%]
电穿孔促进三角褐指藻高效核转化
Chunye Zhang,Hanhua Hu
Chunye Zhang
We established a high-efficiency nuclear transformation method for the diatom Phaeodactylum tricornutum using an electroporation system. Based on a universal electroporation protocol, the conditions for the introduction of exogenous DNA inc...
Influence of moonlight on mRNA expression patterns of melatonin receptor subtypes in the pineal organ of a tropical fish [0.03%]
月光对热带鱼松果体内皮质醇受体亚型基因表达的影响
Yong-Ju Park,Ji-Gweon Park,Yuki Takeuchi et al.
Yong-Ju Park et al.
The goldlined spinefoot, Siganus guttatus, is a lunar-synchronized spawner, which repeatedly releases gametes around the first quarter moon during the reproductive season. A previous study reported that manipulating moonlight brightness at ...
PCR survey of 50 introns in animals: cross-amplification of homologous EPIC loci in eight non-bilaterian, protostome and deuterostome phyla [0.03%]
动物中的50个内含子的PCR调查：非两侧对称、原口和后口动物八个门中同源EPIC位点的交叉扩增
K Gérard,E Guilloton,S Arnaud-Haond et al.
K Gérard et al.
Exon Primed Intron Crossing (EPIC) markers provide molecular tools that are susceptible to be variable within species while remaining amplifiable by PCR using potentially universal primers. In this study we tested the possibility of obtaini...
Bioinformatical analysis of nuclear localisation sequences in penaeid densoviruses [0.03%]
对对虾密集病毒属核定位序列的生物信息学分析
Leigh Owens
Leigh Owens
Nuclear location sequences (NLSs) link proteins to importation molecules for transportation into the nucleus. A bioinformatical search of the penaeid parvoviruses was undertaken to look for NLS. All three ORFs of Penaeus merguiensis densovi...
Protein degradation during the diatom cell cycle: annotation and transcriptional analysis of SCF and APC/C ubiquitin ligase genes in Phaeodactylum tricornutum [0.03%]
蛋白在硅藻细胞周期中的降解：半胱天冬酶和APC/C泛素连接酶基因的注释及转录分析——以三角褐指藻为例
Marie J J Huysman,Cindy Martens,Wim Vyverman et al.
Marie J J Huysman et al.
In every eukaryotic organism, unidirectional cell cycle progression is driven by controlled proteolysis. Here, we present the identification of two ubiquitin ligase complexes in the diatom Phaeodactylum tricornutum, the SCF and APC/C, being...
Sulfide oxidation, nitrate respiration, carbon acquisition, and electron transport pathways suggested by the draft genome of a single orange Guaymas Basin Beggiatoa (Cand. Maribeggiatoa) sp. filament [0.03%]
来自瓜雅basin的一个单一的橙色菌丝体贝格亚托氏硫化菌（暂命名：Cand. Maribeggiatoa）sp.草图基因组所建议的硫化物氧化、硝酸盐呼吸作用、碳获取和电子传递途径
Barbara J MacGregor,Jennifer F Biddle,Christopher Harbort et al.
Barbara J MacGregor et al.
A near-complete draft genome has been obtained for a single vacuolated orange Beggiatoa (Cand. Maribeggiatoa) filament from a Guaymas Basin seafloor microbial mat, the third relatively complete sequence for the Beggiatoaceae. Possible pathw...
Selection of reference genes for reverse transcription quantitative real-time PCR normalization in black rockfish (Sebastes schlegeli) [0.03%]
适用于黑岩鱼（ Sebastes schlegeli ）反转录定量实时荧光 PCR 的内参基因筛选
Ma Liman,Wang Wenji,Liu Conghui et al.
Ma Liman et al.
Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a technique widely used for quantification of mRNA transcription. Data normalization is an indispensable process for RT-qPCR and reference genes are most co...
Next-generation transcriptome profiling reveals insights into genetic factors contributing to growth differences and temperature adaptation in Australian populations of barramundi (Lates calcarifer) [0.03%]
下一代转录组分析揭示了遗传因素对澳大利亚海水鲈生长差异和温度适应性的贡献的见解
James R Newton,Kyall R Zenger,Dean R Jerry
James R Newton
Identification of genetically-regulated adaptation in fish is a precursor to understanding how populations will respond to future climate induced stressors like temperature. Australian populations of barramundi (Lates calcarifer) show stron...
Cloning and expression analysis of nonspecific cytotoxic cell receptor 1 (Ls-NCCRP1) from red snapper (Lutjanus sanguineus) [0.03%]
红海真鲷(Lutjanus sanguineus)非特异性细胞毒效应细胞受体1克隆及表达分析
Jia Cai,Shina Wei,Bei Wang et al.
Jia Cai et al.
It is well known that nonspecific cytotoxic cells (NCCs) are kinds of natural killer cell mediated innate immune responses in teleosts. The nonspecific cytotoxic cell receptor protein 1 (NCCRP-1) is an important cell surface protein on NCC,...
The genetic diversity of the noble scallop (Chlamys nobilis, Reeve 1852) in China assessed using five microsatellite markers [0.03%]
基于5个微卫星核苷酸多态性标记的我国扇贝遗传多样性分析研究
Yanhong Wang,Dingkun Fu,Jianjun Xia
Yanhong Wang
Five highly variable microsatellite loci were used to investigate the genetic diversity and population structure of the noble scallop Chlamys nobilis, in the South China Sea. A total of 200 individual scallops from 4 populations were genoty...