Preparation of a multiple interacting magnetic fluid for rapid and sensitive simultaneous extraction of β-nicotinamide mononucleotide metabolites in biological samples [0.03%]
一种用于快速灵敏地同时提取生物样本中烟酰胺单核苷酸代谢物的多功能磁性流体的制备方法
Yajie Zhang,Zhuhui Chen,Aoli Ye et al.
Yajie Zhang et al.
Background: β-nicotinamide mononucleotide stands out as an essential breakthrough in "anti-aging" and consistently leads the list of top-selling nutritional supplements in terms of quantity. As the metabolites of β-nico...
Spatially lipidomic characterization of patient-derived organoids by whole-mount autofocusing SMALDI mass spectrometry imaging [0.03%]
基于自聚焦SMALDI质谱成像的患者衍生类器官全层脂质组学表征
Chunyan Lan,Ying Peng,Hengtong Zuo et al.
Chunyan Lan et al.
Background: Patient-derived organoids (PDOs) are multi-cellular cultures with specific three-dimensional (3D) structures. Tumor organoids (TOs) offer a personalized perspective for assessing treatment response. However, t...
High-resolution quadrupole improves spectral purity and reduces interference from non-target ions in isobaric multiplexed quantitative proteomics [0.03%]
高分辨率四极杆可提高谱图纯度并减少等重多路复用定量蛋白质组学中的非目标离子干扰
Shen Zhang,J C Yves Le Blanc,Brett Larsen et al.
Shen Zhang et al.
Background: Mass spectrometry (MS)-based proteomics is a powerful tool for identifying and quantifying proteins. However, chimeric spectra caused by the fragmentation of multiple precursors within the same isolation windo...
Advancements in effervescent-assisted dispersive micro-solid phase extraction for the analysis of emerging pollutants [0.03%]
起泡辅助离散微固相萃取分析新兴污染物的研究进展
Nor Munira Hashim,Nurina Izzah Mohd Husani,Niluh Indria Wardani et al.
Nor Munira Hashim et al.
Emerging pollutants pose an increasing threat to the environment and human well-being, requiring substantial progress in analytical methodologies. Dispersive micro-solid phase extraction (μ-dSPE) has proven successful in detecting and meas...
An electrochemiluminescent imaging strategy based on CRISPR/Cas12a for ultrasensitive detection of nucleic acid [0.03%]
一种基于CRISPR/Cas12a的电化学发光成像策略实现核酸的超灵敏检测
Sijian Luo,Jie Wu,Min Zhong et al.
Sijian Luo et al.
Background: Persistent infection with human papillomavirus (HPV) significantly contributes to the development of cervical cancer. Thus, it is urgent to develop rapid and accurate methods for HPV detection. Herein, we pres...
Enzyme- and label-free cascade isothermal amplification aptasensor for the ultrasensitive detection of ochratoxin A [0.03%]
一种超灵敏检测伏马毒素的酶和标记免扩增放大适配体传感器
Ming Li,Yu Xiao,Zexuan Wang et al.
Ming Li et al.
Background: Ultrasensitive detection is crucial for the early warning and intervention of risk factors, ultimately benefiting the environment and human health. Low levels of ochratoxin A (OTA) present a hidden yet signifi...
Highly sensitive ECL detection of miRNA based on self-generated coreactant and target-induced catalyzed hairpin assembly [0.03%]
基于自生成芯反应剂和靶标诱导催化发夹装配的超灵敏微滴定免疫层析法检测miRNA
Xinli Wang,Hongyan Liu,Shaoqiang Zhang et al.
Xinli Wang et al.
Background: Recently, various techniques have been developed to accurately and sensitively detect tumor biomarkers for the early diagnosis and effective therapy of cancer. The electrochemiluminescence (ECL) method holding...
Cellulose-based yellow-green emitting carbon dots with large Stokes shift as effective "turn off-on" fluorescence platforms for Cr (VI) and AA dual efficacy detection [0.03%]
一种基于大斯托克斯位移的黄色-绿色发射碳点，可有效检测Cr（VI）和AA的“关启”型荧光平台
Yan-Feng Li,Xian Zhang,Qian Lu et al.
Yan-Feng Li et al.
Background: Hexavalent chromium (Cr (VI)) is highly carcinogenic to humans. Ascorbic acid (AA) deficiency can be hazardous to health. And the dual-effect fluorescence detection of them is an important research topic. Carb...
Lysine methylation: A strategy to improve in-cell NMR spectroscopy of proteins [0.03%]
组蛋白赖氨酸甲基化提高蛋白质在细胞内的核磁共振分析
Xiong Xiao,Jianhua Zhan,Biao Liu et al.
Xiong Xiao et al.
Background: In-cell NMR is a valuable technique for investigating protein structure and function in cellular environments. However, challenges arise due to highly crowded cellular environment, where nonspecific interactio...
A quantitative method for aquaporin-1 protein using magnetic preconcentration and probe-based immunoassay coupling to inductively coupled plasma mass spectrometry in urine analysis [0.03%]
一种定量分析尿液中水通道蛋白的方法：结合磁性富集和ICPMS免疫分析法
Shuqi Liang,Shuang Zhao,Hongtao Liu et al.
Shuqi Liang et al.
Background: Aquaporin-1 (AQP1) protein plays a crucial role in intracellular and extracellular water homeostasis and fluid transport in organs and tissues associated with diverse life activities and is extremely abundant ...