Colorimetric detection of leucine aminopeptidase based on p-aminophenol-mediated redox cycling between Fe3+-ferrozine and tris(2-carboxyethyl)phosphine [0.03%]
基于对氨基酚介导的Fe3+-萤火虫素与三(2-羧乙基)膦红氧循环的色度法检测谷氨酰胺转氨酶
Congkui Tian,Xiaoning Chu,Changsen Man et al.
Congkui Tian et al.
Background: Leucine aminopeptidase (LAP) is a promising biomarker for the detection and diagnosis of liver diseases encompassing liver cancer and liver injury. Existing assays for LAP activity have limitations, as most of...
Deep intact proteoform quantification of low-microgram-scale proteome by tandem mass tag labeling and online 2D high-pH/low-pH RPLC top-down analysis [0.03%]
基于串联质量标签标记和在线二维反相色谱的顶底结合蛋白质绝对定量研究
Yanting Guo,Trishika Chowdhury,Walter P Galie et al.
Yanting Guo et al.
Isobaric chemical tag labels are the 'gold standard' for quantifying proteins in bottom-up proteomics. Recently, our group developed and optimized an intact protein-level tandem mass tag (TMT) labeling platform to identify and quantify inta...
Conformational changes in a Pseudo[1]catenane induced by ethyl acetate and temperature: A combined HPLC and electronic circular dichroism study [0.03%]
乙酸乙酯和温度对拟[1]_categorical化合物构象变化的影响：高效液相色谱及电子圆二色谱的联合研究
Martina Mazzaferro,Francesca Romana Mammone,Ilenia Pisagatti et al.
Martina Mazzaferro et al.
Background: Stereochemistry plays a pivotal role in the recognition properties of macrocyclic systems bearing multiple stereogenic elements. This study investigates a chiral pseudo[1]catenane based on a pillar[5]arene cor...
Using reverse osmosis to isolate natural organic matter from large samples of fresh water [0.03%]
利用反渗透法从大量淡水样品中分离天然有机物
E Michael Perdue,Yusupha M Sey,A McDonald
E Michael Perdue
Natural organic matter (NOM) has been concentrated from five drinking water sources in Minnesota, Florida, Washington, California, and North Carolina, USA. Reverse osmosis (RO), coupled with cation exchange (CEX-H+) and/or electrodialysis (...
In-situ growth of Eu-doped dual-functional fluorescent MOFs on glass vials: A portable platform for extraction and rapid detection of pyrethroid pesticides residues in tea [0.03%]
原位生长Eu掺杂双功能荧光MOF于玻璃小瓶上：茶叶中拟除虫菊酯类农药残留提取和快速检测的便携式平台
Xiuxiu Wu,Shun Mao,Yanxin Wang et al.
Xiuxiu Wu et al.
Rapid detection of pyrethroid pesticide residues in food is critical to ensure human health. In this work, based on rationally-designed Eu-doped MOFs with dual functions of effective adsorption and fluorescent sensing to pyrethroid pesticid...
Unlocking the chromatographic potential of emerging hydrogen-bonded/metal-organic frameworks as advanced functional materials for silica stationary phase [0.03%]
新兴氢键/金属有机框架的色谱潜力解锁作为硅胶固定相的先进功能材料
Linjun Zuo,Yanjuan Liu,Hongjie Wang et al.
Linjun Zuo et al.
Background: Hydrogen/metal bonded organic frameworks (HOFs/MOFs), with their high porosity and tunable structures, demonstrate broad application prospects as chromatographic separation materials. However, MOF particles of...
Cell micropatterning using an indium-tin-oxide patterned microarray chip [0.03%]
使用图案化微阵列芯片进行细胞微模式培养
Hidetaka Ueno,Shohei Yamamura
Hidetaka Ueno
Cell micropatterning is an essential technology for elucidating intercellular crosstalk and the behavior of single cells and cell populations. However, conventional micropatterning methods based on chemically modified cell-adhesive and nona...
CRISPR/Cas12a and CHA-based SERS platform for ultrasensitive nucleic acid detection [0.03%]
基于CRISPR/Cas12a和CHA的SERS平台用于超灵敏核酸检测
Huimin Wang,Chengxin Bao,Lin Liu et al.
Huimin Wang et al.
Background: Highly sensitive nucleic acid detection is essential for analytical applications. Conventional methods often require complex pre-amplification procedures, limiting their practical utility in screening. Develop...
Interface-enhanced label-free FeOOH-mesoporous TiO2 nanocomposite biosensor for highly sensitive quantitative exosome detection to assist cancer screening [0.03%]
用于高灵敏定量检测外泌体以辅助癌症筛查的界面增强型无标记FeOOH-介孔TiO2纳米复合材料生物传感器
Bokai Zhou,Shuyan Liu,Xu Yin et al.
Bokai Zhou et al.
Exosome-based liquid biopsy has shown great promise in cancer diagnosis. Electrochemical biosensors, owing to their high efficiency, sensitivity, and operational simplicity, have become powerful tools for biological sample analysis. In this...
Intracellular imaging and therapeutic application of liver fibrosis via a microRNA-on self-stacking amplification circuit [0.03%]
基于microRNA-on自堆叠放大回路的肝纤维化细胞内成像及治疗应用研究
Xiao-Meng Sun,Yin-Xiang Chen,Lin-Ru Xu et al.
Xiao-Meng Sun et al.
Background: Liver fibrosis is a major global health issue with limited treatment options. Activation of hepatic stellate cells (HSCs) induces collagen deposition, which is a core step in the progression of liver fibrosis....