A safety and absolute activity measurement method for Phi29 DNA polymerase based on chemiluminescent detection of dATP consumption [0.03%]
基于化学发光检测dATP消耗的Phi29 DNA聚合酶安全性和绝对活性测定方法
Xuefeng Wang,Ping Gui,Xiao Li et al.
Xuefeng Wang et al.
Background: Phi29 DNA polymerase serves as a cornerstone enzyme in molecular biology, enabling critical applications such as rolling-circle amplification, multiple strand-displacement amplification, and single-molecule re...
Pixel array-based urine biosensor for detection of trimethylamine N-oxide and glucose for early detection of diabetic kidney disease [0.03%]
一种基于像素阵列的尿液生物传感器用于检测三甲胺N-氧化物和葡萄糖以期实现糖尿病肾脏疾病的早期发现
Mei-Ching Yu,Chi-Jen Lo,Wei-Cheng Lin et al.
Mei-Ching Yu et al.
Trimethylamine N-oxide (TMAO) serves as a crucial biomarker for early detection and prevention of cardiovascular and chronic kidney diseases. In this study, we design and implement a novel pixel array-based urine biosensor to explore the re...
Development of ultra-miniaturized weak affinity chromatography coupled to mass spectrometry as a high throughput fragment screening method against wild-type and purified membrane proteins embedded in biomimetic membranes [0.03%]
超小型弱亲和色谱与质谱联用技术作为高通量片段筛选方法的发展：用于野生型及纯化膜蛋白在仿生膜中的筛选
François-Xavier Vidal,Julie Gil,Maud Gregson et al.
François-Xavier Vidal et al.
Background: Membrane proteins, which make up approximately 30 % of the proteome, are important drug targets but present many challenges in drug discovery, including limited production rates, low final yields of pure and f...
AIE-based dual-ratiometric chemosensor for detection of sulfur dioxide derivatives and viscosity and its application in food samples and biological imaging [0.03%]
基于AIE的用于检测硫化物和粘度的双比值化学传感器及其在食品样品和生物成像中的应用
Na Tang,Ruian Sun,Kangshuo Xu et al.
Na Tang et al.
Background: SO2 is an important gas molecular messenger that participates in many physiological activities and helps maintaining the redox homeostasis in biological system. Especially, SO2 can effectively regulate oxidati...
Blood detection of autoimmune encephalitis based on laser-induced breakdown spectroscopy and Raman spectroscopy [0.03%]
基于激光诱导击穿光谱和拉曼光谱的自身免疫性脑炎血液检测方法
Zhifang Zhao,Wangshu Xu,Geer Teng et al.
Zhifang Zhao et al.
Background: Recently, the incidence range of autoimmune encephalitis (AE) in people has rapidly expanded, and the diagnosis procedure of clinical criteria for AE remains complicated. Herein, with advantages of rapid speed...
Multiscale workflow for the profiling and identification of urinary food bioactives metabolites Part I: Optimizing urine extraction [0.03%]
尿液食物生物活性代谢产物分析鉴定的多尺度工作流程 第一部分 优化样品前处理方法
Evangelos Kalampokis,Theodora Nikou,Stavros Beteinakis et al.
Evangelos Kalampokis et al.
Background: The relationship between diet, human health, and disease prevention is well-established, with food bioactive compounds (FBs) widely recognized for their beneficial effects. Metabolism is key in transforming pr...
Electrochemistry sensing of ascorbic acid based on conductive metal-organic framework (Cu3(benzenehexathiol)) nanosheets modified electrode [0.03%]
基于导电金属有机骨架(Cu3(benzenehexathiol)纳米片修饰电极的电化学检测维生素C传感器研究
Runhao Yuan,Xiaolong Zhong,Weiming Sun et al.
Runhao Yuan et al.
Background: Bulk-type conductive metal-organic frameworks (c-MOFs) had been applied to modify the electrode and used in electrochemical sensing because of the high conductive properties. But which still suffer from low ma...
Enhanced SERS performance through defect-guided growth of 2D/3D AuAg nanoplates for chemical sensing and cellular imaging applications [0.03%]
缺陷引导生长的二维/三维AuAg纳米板的增强型SERS性能在化学传感和细胞成像中的应用研究
Kuan-Wen Liu,Pei-Yu Sie,Hsi-Ying Chen et al.
Kuan-Wen Liu et al.
Background: Surface-enhanced Raman scattering (SERS) is a powerful analytical technique that utilizes localized electromagnetic fields to amplify molecular vibrational signatures. The effectiveness of SERS substrates reli...
Stable isotope tracing reveals glucose metabolism characteristics of drug-resistant B-cell acute lymphoblastic leukemia [0.03%]
稳定同位素示踪揭示药物难治性B细胞急性淋巴细胞白血病糖代谢特征
Rong Hu,Zhengwei Duan,Mengyao Wang et al.
Rong Hu et al.
Background: Adult B-cell acute lymphocytic leukemia (B-ALL) is a malignant hematologic tumor characterized by the uncontrolled proliferation of B-cell lymphoblasts in the bone marrow. Despite advances in treatment, includ...
Facile preparation of isolation columns filled with integral hybrid materials for efficient isolation of extracellular vesicles from microliter sample [0.03%]
一种用于从微升样品中高效分离细胞外囊泡的集成混合材料填充隔离柱的简易制备方法
Yujie Lin,Mengxi Chen,Yuanyuan Yu et al.
Yujie Lin et al.
Background: Extracellular vesicles (EVs) and their anomalously altered cargoes represent a promising avenue for clinical diagnostics and prognostics. A critical challenge in EV research is the efficient isolation of these...