Exponential rolling circle amplification-hybridization chain reaction (EXRCA-HCR) for AgNPs@gel-enhanced fluorescence ultrasensitive detection of miRNA-21 [0.03%]
基于银纳米颗粒@凝胶增强荧光的指数滚环扩增-杂交链式反应 ultra miRNA-21检测技术(EXRCA-HCR)
Wenjiao Zhang,Yuchen Song,Dongmei Deng et al.
Wenjiao Zhang et al.
MicroRNA (miRNA) is a common tumor marker, whose abnormal expression is often closely related to the occurrence of various diseases. However, the conventional method for detecting miRNA is qRT-PCR, requiring additional reverse transcription...
Rapid filamentous fungi gene knockout identification through high-throughput droplet microfluidics [0.03%]
基于高通量微滴流体学的丝状真菌基因敲除快速鉴别法
Yuwen Li,Jing Dai,Huan Zhang et al.
Yuwen Li et al.
Fungi are a diverse group of eukaryotic organisms, with over 1.5 million species inhabiting ecosystems worldwide. Many fungi grow as filaments (hyphae) and play critical ecological roles, both beneficial and harmful. Understanding their fun...
γ-cyclodextrin metal-organic framework-activated gold nanoclusters with water solubility as multi-enzyme mimics for simultaneous detection of pesticide and cholinesterase activity [0.03%]
γ-环糊精金属有机骨架活化金纳米团簇（溶于水），同时检测农药和胆碱酯酶活性的多酶模拟物
Bing Tan,Qian Wang,Qiaoli Liu et al.
Bing Tan et al.
Cyclodextrin metal-organic framework (CD-MOF) with environmentally friendly components has attracted enormous attention in the past few decades. However, the fragile aqueous stability has limited its application in aqueous catalysis. In thi...
A novel chemiresistive biosensor utilizing green-synthesized AgNPs and MWCNT-ZnO nanofibers for rapid detection of tuberculosis Lipoarabinomannan (LAM) antigen [0.03%]
一种利用绿色合成的AgNPs和MWCNT-ZnO纳米纤维的新型化学电阻式生物传感器，用于快速检测结核病脂阿拉伯甘聚糖（LAM）抗原
Dinesh R Rotake,Jitendra B Zalke,Shubham C Anjankar et al.
Dinesh R Rotake et al.
Background: Tuberculosis (TB) poses a major global health threat, especially in low- and middle-income countries with limited diagnostic infrastructure. Early and accurate detection is essential to control its spread and ...
A minimally instrumented method for the detection of rifampicin resistance-causing mutations in Mycobacterium tuberculosis utilizing lateral flow readout [0.03%]
一种检测结核分枝杆菌利福平耐药突变的极简型方法（采用侧向流动读取）
Ayushi Chauhan,S SivaKumar,Bhushan J Toley
Ayushi Chauhan
Background: Genotypic methods for detecting antimicrobial resistance in Mycobacterium tuberculosis (M.tb) typically require expensive instrumentation, limiting their accessibility in peripheral and resource-limited settin...
Ultrasensitive TALEs-mediated electrochemical sensor for DNA methylation detection based on RCA reaction and Exo III cyclic amplification-assisted "silver-link" crossing electrode [0.03%]
基于滚环扩增和Exo III循环放大辅助“银链接”交叉电极的超灵敏TALE介导的用于DNA甲基化检测的电化学生物传感器
Jialin Zhang,Mengyu Kang,Jinhui Li et al.
Jialin Zhang et al.
Pancreatic cancer has become one of the most challenging malignant tumors. This type of cancer shows a high mortality paralleling morbidity and a rather low 5-year survival rate. Early diagnosis of the disease is the key to prevent and trea...
Enhanced microextraction kinetics: Sorptive-dispersive vs. conventional techniques on rotating disks [0.03%]
增强的微提取动力学：旋转盘上的吸附-分散与常规技术比较研究
Nicolás Morales,Alejandra Molina-Balmaceda,Daniel Arismendi et al.
Nicolás Morales et al.
Background: A new approach based on Stir Bar Sorptive Dispersive Microextraction (SBSDME), termed Rotating Disk Sorptive Dispersive Extraction (RDSDE), was developed to confirm and quantify the kinetic advantages of sorbe...
An integrated non-alkaline derivatization strategy using 2,2'-dithiodipyridine and HPLC for simultaneous analysis of total and low-molecular-weight free thiols in human serum [0.03%]
一种集成的非碱衍生化策略，采用2,2'-二噻基吡啶和HPLC同时分析人血清中总低分子量游离硫醇
Zhiyu Shao,Jie Zeng,Jun Dong et al.
Zhiyu Shao et al.
Background: Oxidative stress is a major mechanism underlying aging and health damage, and it is associated with decreased serum free thiol levels. Monitoring serum free thiol levels provides a valuable reference for asses...
Nasopharyngeal cancer screening and immunotherapy efficacy evaluation based on plasma separation combined with label-free SERS technology [0.03%]
基于血浆分离结合无标签SERS技术的鼻咽癌筛查及免疫治疗疗效评价研究
Ruiying Lin,Zhangying Jiang,Jinyong Lin et al.
Ruiying Lin et al.
Background: In recent years, significant progress has been made in the treatment of nasopharyngeal carcinoma (NPC). The application of immunotherapy, especially the use of Programmed Cell Death Protein 1 inhibitors, has d...
Multi-interaction (substituted-phenylcarbamate)-hydroxypropyl-β-cyclodextrin chiral stationary phases for liquid chromatography [0.03%]
用于液相色谱的手性固定相（取代的芳基碳酸盐）-羟丙基-β-环糊精
Junchao Zhu,Linna Xu,Wentao Xu et al.
Junchao Zhu et al.
Background: (Substituted-phenylcarbamate)-β-cyclodextrin (β-CD) chiral stationary phases (CSPs) showed high enantioselectivity and complementary capabilities compared to polysaccharide-based CSPs in liquid chromatograph...