SERS-based lateral flow immunoassay for rapid and sensitive sensing of nucleocapsid protein toward SARS-CoV-2 screening in clinical samples [0.03%]
基于SERS的横向流动免疫测定法，快速灵敏地检测临床样本中的SARS-CoV-2核衣壳蛋白
Weixia Zhi,Lingwei Wang,Li Dai et al.
Weixia Zhi et al.
Early and accurate identification of SARS-CoV-2 infection is crucial for epidemic prevention and control. Lateral flow immunoassay (LFIA) has become the mainstream method for screening SARS-CoV-2 infection due to its rapid, simple and amena...
Rapid baseline hump-free analysis of therapeutic proteins in a wide molecular weight range by SDS - capillary agarose gel electrophoresis [0.03%]
宽分子量范围治疗蛋白基线分析的毛细管聚丙烯酰胺凝胶电泳快速无驼峰法
Dániel Sárközy,Anna Farkas,András Guttman
Dániel Sárközy
Background: Sodium dodecyl sulfate capillary gel electrophoresis traditionally employs entangled polymer networks (CE-SDS) or borate cross-linked gels (SDS-CGE) for size-based protein analysis. However, the separation of ...
Using CE to confirm the activity of fluorescent miRFP670-LIMK1 protein produced for MST assays directly in cell lysate [0.03%]
利用CE直接确认用于MST测定的荧光miRFP670-LIMK1蛋白在细胞裂解液中的活性
Solweig Chartier,Bérengère Claude,Rouba Nasreddine et al.
Solweig Chartier et al.
LIMK1 is a promising therapeutic target for multiple pathologies. Its holistic study seems crucial, as none of its inhibitors has passed clinical trials. The need for new fluorescent probes for the study of enzyme/ligand interactions is alw...
Integrating click chemistry into protein expression for efficient preparation of immobilized protein bio-surface with enhanced stability and reproducibility in affinity chromatography [0.03%]
基于点击化学的固定化亲和层析用重组蛋白高效制备及稳定性研究
Xue Zhao,Yunshan Wang,Jiatai Yin et al.
Xue Zhao et al.
Background: Protein immobilization method play a pivotal role in developing affinity chromatographic assays for both basic and applied research, and site-specific, covalent attachment serves as the most desirable strategy...
Red-emitting aggregation-induced emission fluorescent probe for monitoring fluctuation of HClO in mitochondria during ferroptosis [0.03%]
一种用于铁死亡期间线粒体内HOCl波动监测的红色发射聚集诱导发光型荧光探针
Bin Yang,Wangbo Qu,Jiao Lu et al.
Bin Yang et al.
Background: Ferroptosis is an iron-dependent programmed cell death pathway driven by lipid peroxidation that involves various inflammation-related diseases and even cancer, which is often accompanied by the accumulation o...
Dual-modal biosensor based on proton-modulated mechanism integrated nanozyme for acetylcholinesterase analysis [0.03%]
基于质子调节机制集成纳米酶的双模生物传感器用于乙酰胆碱酯酶检测
Xueli Jin,Ying Shao,Sixuan Zhu et al.
Xueli Jin et al.
Background: Acetylcholinesterase (AChE) not only serves as an important biomarker for nervous system diseases, but also facilitates the discovery of related drugs. Therefore, the sensitive and accurate detection of AChE i...
Novel strategy for screening aptamers of Staphylococcus aureus enterotoxin A based on active fragments and fusion design [0.03%]
基于活性片段和融合设计的新颖金黄色葡萄球菌海藻毒素A适配体筛选策略
Shujing Ge,Mengyao Guan,Fei Pan et al.
Shujing Ge et al.
Background: Staphylococcus aureus enterotoxin A (SEA) is the major toxin responsible for food poisoning caused by Staphylococcus aureus infection. Aptamers are an ideal solution for visual detection of SEA. However, scree...
Flip-PAD integrating laser-scribed platinum-nanozyme for rapid smartphone-based colorimetric determination of ascorbic acid [0.03%]
基于智能手机的快速荧光试纸条测定维生素C含量
Annalisa Scroccarello,Flavio Della Pelle,Selene Fiori et al.
Annalisa Scroccarello et al.
Background: The development of portable easy-to-use devices to selectively determine antioxidants still represents an open issue; antioxidants, in fact, often coexist and have similar redox reactivity. In this framework, ...
How excitation-emission matrices have revolutionised multi-way data analyses - A tutorial for multi-way EEM-based calibration method developments [0.03%]
激发发射矩阵如何彻底改变了多维数据分析-一种基于EEM的校正方法发展的教程式研究
Marina Antonio,Arsenio Muñoz de la Peña,Héctor C Goicoechea et al.
Marina Antonio et al.
For many years, excitation-emission matrix (EEM) fluorescence spectroscopy has been a compelling analytical technique, fostering the development of multidimensional data analysis-based methods. Its applications span diverse fields, includin...
Target inhibition of rolling circle amplification generates G43 long nanowires for reverse signaling determination of alkaline phosphatase [0.03%]
目标抑制滚环放大生成G43长纳米线用于碱性磷酸酶的反向信号测定
Shuyu Zhu,Shiying Zhou,Liyuan Deng et al.
Shuyu Zhu et al.
Background: Alkaline phosphatase (ALP) can catalyze the hydrolysis of phosphate esters under alkaline conditions, mediate the dephosphorylation of substances such as proteins and nucleic acids. ALP can participate in phys...