Tetrahedral framework DNA-aptamer clicked affinity monolith with enhanced specificity for online profiling of Okadaic acid [0.03%]
一种特异性增强的 tetrahedral 框架DNA适配器点击亲和单柱用于实时检测石房蛤毒素
Qi Peng,Dexin Luo,Chengqun Chen et al.
Qi Peng et al.
Developing high-performance aptamer-mediated affinity monolith represent a powerful strategy for highly efficient recognition and online precise target profiling (e.g. shellfish toxin). However, challenges including spontaneous aggregation ...
Analysis of polybrominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs) in air emission samples by GCHRMS: method development, validation and a real-case application [0.03%]
采用GC-HRMS分析空气样品中的多溴代二苯并对二噁英和呋喃(PBDD/F)：方法开发、验证及实际案例应用
Albert Sales-Alba,Xavier Ortiz Almirall,Jordi Diaz-Ferrero
Albert Sales-Alba
Polybrominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs) are emerging dioxin-like pollutants formed during bromine-containing thermal processes, yet not harmonized analytical methods currently exist for their determination in atmospheri...
Investigation of adsorption behavior and scale up for hydroxysafflor yellow A purification from Safflower: a case study [0.03%]
羟基番红黄色素从红花中吸附行为及放大研究：一例研究
Amin Tabesh,Hassan Rezadoost,Masoud Rahimi et al.
Amin Tabesh et al.
Given the complexity of natural extracts, multiple purification steps are often required to satisfy strict purity requirements for the isolated product. In this context, mathematical modeling could be a useful tool to evaluate the feasibili...
Uncertainty-driven model-based search methods for method development in liquid chromatography [0.03%]
基于不确定性的模型驱动的液相色谱方法开发中的搜索方法研究
Leon E Niezen,Deirdre Cabooter,Gert Desmet
Leon E Niezen
Empirical retention modelling is a proven method development approach that uses a limited set of initial "scanning" experiments to build analyte retention models and predict optimal experimental conditions from a predicted chromatographic r...
Synthesis of C18-modified radial mesoporous core-shell silica microspheres with tunable shell thickness for ultra-high performance liquid chromatography [0.03%]
一种用于超高效液相色谱的C18功能化介孔核壳硅微球的合成及其壳层厚度的调控
Renxiu Huang,Yali Yang,Yue Ming et al.
Renxiu Huang et al.
With the advent of ultra-high performance liquid chromatography (UHPLC), core-shell silica microspheres (CSSMs) have emerged to balance system backpressure and column efficiency. As one of the methods for preparing CSSMs, the biphasic synth...
Fabrication of trypsin-immobilized metal-organic framework-polymer monolithic spin column through a facile biomineralization strategy for efficient proteome digestion [0.03%]
一种通过简便的生物矿化策略制备胰蛋白酶金属有机骨架聚合物整体柱用于有效蛋白质组酶解的新方法
Shengman Zhang,Ke Xu,Bingjie Li et al.
Shengman Zhang et al.
In this work, a novel trypsin-immobilized metal-organic framework (MOF)-polymer monolithic spin column was prepared by a facile biomineralization strategy, which intelligently utilizes the monolith to provide a porous support and the MOF to...
An isotopologue-mixture-based internal standard strategy for LC-MS/MS quantification in plasma [0.03%]
基于同位素内标的LC-MS/MS定量方法在血浆中的应用
Ping Guo,Yuan Liu,Guozhu Liu et al.
Ping Guo et al.
Stable isotope-labeled internal standards (SIL-IS) are the gold standard for liquid chromatography-tandem mass spectrometry (LC-MS/MS) quantification in complex biological matrices. Their application can be limited by synthetic complexity, ...
Establishment of an integrated profiling-fingerprinting-chemometrics strategy for quality control and mechanistic investigation of Fuhuo Capsule [0.03%]
建立综合的特征谱集-化学计量策略控制独活胶囊质量并探讨其作用机理
Shuang Liang,Jie Yang,Jiachen Ge et al.
Shuang Liang et al.
Fuhuo Capsule is widely prescribed for the treatment of osteonecrosis of the femoral head; however, its chemical composition and reliable quality markers have not been comprehensively elucidated. In this work, comprehensive chemical profili...
Quantification of residual protein from biocatalysts employed in the synthesis of pharmaceutical drug substances using reversed-phase HPLC [0.03%]
采用反相高效液相色谱法测定生物催化剂在合成药物活性物质过程中残留蛋白量
Matthew R Swoyer,Kaitlyn M Flynn,Timothy A Brown et al.
Matthew R Swoyer et al.
Biocatalysis, the use of enzymes to catalyze chemical transformations, is becoming an increasingly important focus in the manufacture of pharmaceutical drug substances. The use of biocatalysts lends marked improvements in process efficiency...
Porous organic framework materials for the separation of natural products: A comparative study of separation mechanisms in metal-organic frameworks and covalent organic frameworks [0.03%]
用于天然产物分离的多孔有机骨架材料：金属有机框架和共价有机框架中分离机制的比较研究
Ting Zhu,Xianyu Li,Bing Liu et al.
Ting Zhu et al.
The efficient isolation and purification of natural products are critical for fundamental research, drug discovery, and quality control. However, the complexity of sample matrices and the structural diversity of target analytes present sign...