Strategies to Enhance Stability of Cryopreservation Processes for Cell-Based Products [0.03%]
提高细胞基产品冷冻保存过程稳定性的策略
Yuki Uno,Yusuke Hayashi,Hirokazu Sugiyama et al.
Yuki Uno et al.
The projected expansion of the global market for cell manufacturing, which contributes to regenerative medicine and cell therapies, warrants the designing and development of scalable cryopreservation processes for cell-based products (CBPs)...
Capitalizing on mechanistic insights to power design of future-ready intracellular optogenetics tools [0.03%]
基于机理的新一代细胞内光遗传工具的设计与开发
Si Bin Chew,Emily Harjabrata,Cameron Jing Han Goh et al.
Si Bin Chew et al.
Intracellular optogenetics represents a rapidly advancing biotechnology that enables precise, reversible control of protein activity, signaling dynamics, and cellular behaviours using genetically encoded, light-responsive systems. Originall...
Machine learning methods for small data and upstream bioprocessing applications: A comprehensive review [0.03%]
小数据和上游生物工艺应用的机器学习方法综述
Johnny Peng,Thanh Tung Khuat,Katarzyna Musial et al.
Johnny Peng et al.
Data are crucial for machine learning (ML) applications, yet acquiring large datasets can be costly and timeconsuming, especially in complex, resource-intensive fields like biopharmaceuticals. A key process in this industry is upstream biop...
Harnessing spatial transcriptomics to understand host-parasite interactions in plants and animals [0.03%]
利用空间转录组学理解植物和动物中宿主-寄生虫互作
Anna Pijnacker,Christine W Bruggeman,Hendrik C Korswagen et al.
Anna Pijnacker et al.
Obligate parasites pose a significant threat to animal, human, and plant health by affecting host gene expression through mechanisms that are poorly understood. Spatial transcriptomic technologies are revolutionizing our understanding of an...
Engineering strategies for microbial synthesis, customized modification, and application of hemoglobin [0.03%]
工程策略驱动的微生物血红蛋白合成、定制化修饰及应用改造
Fan Liu,Chunxiang Feng,Zirui Yin et al.
Fan Liu et al.
Hemoglobin is a functional protein with heme as a cofactor, playing a crucial role in transporting oxygen and maintaining nitric oxide metabolic balance. Besides its physiological functions, hemoglobin has broad potential applications in me...
Optimizing single and cascade microbial enzyme systems through site-directed mutagenesis for enhancing mycotoxin detoxification [0.03%]
通过定向进化优化单酶和级联酶系统以增强霉菌毒素解毒效能
Tosin Victor Adegoke,Sifan Lu,Ogedegbe Gloria Adegoke et al.
Tosin Victor Adegoke et al.
Mycotoxins, among the most extensively studied biological toxins, pose significant health risks to humans and animals, causing substantial economic losses in the agricultural sector. Numerous conventional enzymes isolated from microorganism...
Ying-Ying Xu,Sheng-Mei Zhou,Lu-Yan Wang et al.
Ying-Ying Xu et al.
The CRISPR/Cas9 system has emerged as a revolutionary tool for gene editing, widely used in the biomedical field due to its simplicity, efficiency, and cost-effectiveness. However, evidence suggests that CRISPR/Cas9 can induce off-target ef...
Precise control of transcriptional stoichiometry in bacteria: From mechanisms to synthetic biology applications [0.03%]
细菌转录比例的精准调控：从机制到合成生物学应用
Duodong Wang,Na Wang,Houhui Song et al.
Duodong Wang et al.
Bacteria exhibit remarkable precision in controlling the stoichiometry of protein subunits within metabolic pathways and macromolecular complexes-a requirement for optimal function and fitness. This review explores the RNA-level mechanisms ...
Cuifang Ye,Xiaoqian Li,Tao Liu et al.
Cuifang Ye et al.
Peroxisome engineering in yeast has emerged as a promising strategy for biomanufacturing, as it enables the compartmentalization of biosynthetic pathways and thus alleviates key bottlenecks in natural product biosynthesis. By sequestering s...
Advances in biosensors for microbial biosynthesis of amino acids and their derivatives [0.03%]
微生物合成氨基酸及其衍生物的生物传感器研究进展
Huina Dong,Tingting Li,Xinyi Ren et al.
Huina Dong et al.
Amino acids and their derivatives play pivotal roles across diverse fields including biotechnology, pharmaceuticals, agriculture, and industrial manufacturing. The development of high-throughput screening methods for strains producing amino...