Agarose degradation for utilization: Enzymes, pathways, metabolic engineering methods and products [0.03%]
琼脂糖降解的利用：酶、途径、代谢工程方法和产物
Chengcheng Jiang,Zhen Liu,Danyang Cheng et al.
Chengcheng Jiang et al.
Red algae are important renewable bioresources with very large annual outputs. Agarose is the major carbohydrate component of many red algae and has potential to be of value in the production of agaro-oligosaccharides, biofuels and other ch...
Molecularly imprinted polymers for the selective recognition of microorganisms [0.03%]
用于特异性识别微生物的分子印迹聚合物
Kamaran Khurshid Dar,Shengnan Shao,Tianwei Tan et al.
Kamaran Khurshid Dar et al.
Molecularly imprinted polymers (MIPs) emerged half a century ago have now attracted tremendous attention as artificial receptors or plastic antibodies. Although the preparation of MIPs targeting small molecules, peptides, or even proteins i...
Philip M Stanley,Lisa M Strittmatter,Alice M Vickers et al.
Philip M Stanley et al.
While DNA's perpetual role in biology and life science is well documented, its burgeoning digital applications are beginning to garner significant interest. As the development of novel technologies requires continuous research, product deve...
What do we learn from enzyme behaviors in organic solvents? - Structural functionalization of ionic liquids for enzyme activation and stabilization [0.03%]
有机溶剂中酶的行为能告诉我们什么？--离子液体的结构功能化在酶激活和稳定中的应用
Hua Zhao
Hua Zhao
Enzyme activity in nonaqueous media (e.g. conventional organic solvents) is typically lower than in water by several orders of magnitude. There is a rising interest of developing new nonaqueous solvent systems that are more "water-like" and...
Joseph Emerson,Bo Kara,Jarka Glassey
Joseph Emerson
The emergence of cell gene therapy (CGT) as a safe and efficacious treatment for numerous severe inherited and acquired human diseases has led to growing interest and investment in new CGT products. The most successful of these have been au...
Expansion of human mesenchymal stem/stromal cells (hMSCs) in bioreactors using microcarriers: lessons learnt and what the future holds [0.03%]
生物反应器中微载体对人源间充质干细胞的扩增：总结经验展望未来
P Silva Couto,M C Rotondi,A Bersenev et al.
P Silva Couto et al.
Human mesenchymal stem/stromal cells (hMSCs) present a key therapeutic cellular intervention for use in cell and gene therapy (CGT) applications due to their immunomodulatory properties and multi-differentiation capability. Some of the indi...
Next-generation metabolic engineering approaches towards development of plant cell suspension cultures as specialized metabolite producing biofactories [0.03%]
下一代代谢工程方法推动植物细胞悬浮培养物作为专门代谢物生产生物反应器的发展
Sagar S Arya,James E Rookes,David M Cahill et al.
Sagar S Arya et al.
Plant cell suspension culture (PCSC) has emerged as a viable technology to produce plant specialized metabolites (PSM). While Taxol® and ginsenoside are two examples of successfully commercialized PCSC-derived PSM, widespread utilization o...
Engineering Staphylococcal Protein A for high-throughput affinity purification of monoclonal antibodies [0.03%]
工程改造葡萄球菌蛋白质A以实现单克隆抗体的高通量亲和纯化
Vinod Amritkar,Satish Adat,Vijay Tejwani et al.
Vinod Amritkar et al.
Protein A chromatography is one of the most widely used purification steps in the manufacturing of the various classes of recombinant and non-recombinant antibodies. Due to the higher cost, lower binding capacity, and limited life cycle of ...
Phosphorylase-catalyzed bottom-up synthesis of short-chain soluble cello-oligosaccharides and property-tunable cellulosic materials [0.03%]
磷酸酶催化短链可溶性纤维寡糖及性能可控的纤维素材料的底向上合成过程
Bernd Nidetzky,Chao Zhong
Bernd Nidetzky
Cellulose-based materials are produced industrially in countless varieties via top-down processing of natural lignocellulose substrates. By contrast, cellulosic materials are only rarely prepared via bottom up synthesis and oligomerization-...
Simone Savino,Marco W Fraaije
Simone Savino
Carbohydrates are widely abundant molecules present in a variety of forms. For their biosynthesis and modification, nature has evolved a plethora of carbohydrate-acting enzymes. Many of these enzymes are of particular interest for biotechno...