Quantifying the impact of cell culture media on CHO cell growth and protein production [0.03%]
测定培养基对CHO细胞生长和蛋白表达的影响
Michelle Combe,Stanislav Sokolenko
Michelle Combe
In recombinant protein production, cell culture media development and optimization is typically seen as a useful strategy to increase titer and cell density, reduce by-products, as well as improve product quality (with cell density and tite...
Non-viral transfection technologies for next-generation therapeutic T cell engineering [0.03%]
下一代治疗性T细胞工程的非病毒转染技术
Laurens Raes,Stefaan C De Smedt,Koen Raemdonck et al.
Laurens Raes et al.
Genetically engineered T cells have sparked interest in advanced cancer treatment, reaching a milestone in 2017 with two FDA-approvals for CD19-directed chimeric antigen receptor (CAR) T cell therapeutics. It is becoming clear that the next...
Synthetic biology-inspired strategies and tools for engineering of microbial natural product biosynthetic pathways [0.03%]
合成生物学理念、策略和工具在微生物次级代谢产物生物合成途径工程中的应用
Khorshed Alam,Jinfang Hao,Youming Zhang et al.
Khorshed Alam et al.
Microbial-derived natural products (NPs) and their derivative products are of great importance and used widely in many fields, especially in pharmaceutical industries. However, there is an immediate need to establish innovative approaches, ...
Jorge João,João Lampreia,Duarte Miguel F Prazeres et al.
Jorge João et al.
Bacteriophages, or simply phages, are the most abundant biological entities on Earth. One of the most interesting characteristics of these viruses, which infect and use bacteria as their host organisms, is their high level of specificity. S...
The emerging role of cellular post-translational modifications in modulating growth and productivity of recombinant Chinese hamster ovary cells [0.03%]
细胞翻译后修饰在调控基因工程中国仓鼠卵巢细胞生长和产量中的作用
Laura Bryan,Martin Clynes,Paula Meleady
Laura Bryan
Chinese hamster ovary (CHO) cells are one of the most commonly used host cell lines used for the production human therapeutic proteins. Much research over the past two decades has focussed on improving the growth, titre and cell specific pr...
Surrogate broodstock to enhance biotechnology research and applications in aquaculture [0.03%]
Aquaculture生物技术研究与应用的供体亲鱼育苗技术
Ye Hwa Jin,Diego Robledo,John M Hickey et al.
Ye Hwa Jin et al.
Aquaculture is playing an increasingly important role in meeting global demands for seafood, particularly in low and middle income countries. Genetic improvement of aquaculture species has major untapped potential to help achieve this, with...
Proteomic and Glyco(proteo)mic tools in the profiling of cardiac progenitors and pluripotent stem cell derived cardiomyocytes: Accelerating translation into therapy [0.03%]
心肌前体和多能干细胞来源的心肌细胞谱系特异性蛋白质组学和糖蛋白组学技术：促进其向治疗的转化
Maria J Sebastião,Lara Marcos-Silva,Patrícia Gomes-Alves et al.
Maria J Sebastião et al.
Research in stem cells paved the way to an enormous amount of knowledge, increasing expectations on cardio regenerative therapeutic approaches in clinic. While the first generation of clinical trials using cell-based therapies in the heart ...
Algae biostimulants: A critical look at microalgal biostimulants for sustainable agricultural practices [0.03%]
微藻生物刺激剂：可持续农业实践中的微藻生物刺激剂的批判性研究 взгляд на биостимуляторы микроалге для устойчивых сельскохозяйственных практик
Rahul Vijay Kapoore,Eleanor E Wood,Carole A Llewellyn
Rahul Vijay Kapoore
For the growing human population to be sustained during present climatic changes, enhanced quality and quantity of crops are essential to enable food security worldwide. The current consensus is that we need to make a transition from a petr...
Jiyoung Shim,Chuqing Zhou,Ting Gong et al.
Jiyoung Shim et al.
The recent development of synthetic biology has expanded the capability to design and construct protein networks outside of living cells from the bottom-up. The new capability has enabled us to assemble protein networks for the basic study ...
Self-assembling peptide-based hydrogels: Fabrication, properties, and applications [0.03%]
基于肽的自组装水凝胶：制备、性质及应用
Kun Fu,Hanguang Wu,Zhiqiang Su
Kun Fu
The hierarchical formation of self-assembling peptide-based hydrogels (SAPHs) starts from peptide to nanofibers, following with the entanglement into hydrogels with nanofibrous network. Such characteristic structure and extraordinary biocom...