Harnessing spatial transcriptomics to understand host-parasite interactions in plants and animals [0.03%]
利用空间转录组学理解植物和动物中宿主-寄生虫互作
Anna Pijnacker,Christine W Bruggeman,Hendrik C Korswagen et al.
Anna Pijnacker et al.
Obligate parasites pose a significant threat to animal, human, and plant health by affecting host gene expression through mechanisms that are poorly understood. Spatial transcriptomic technologies are revolutionizing our understanding of an...
Engineering strategies for microbial synthesis, customized modification, and application of hemoglobin [0.03%]
工程策略驱动的微生物血红蛋白合成、定制化修饰及应用改造
Fan Liu,Chunxiang Feng,Zirui Yin et al.
Fan Liu et al.
Hemoglobin is a functional protein with heme as a cofactor, playing a crucial role in transporting oxygen and maintaining nitric oxide metabolic balance. Besides its physiological functions, hemoglobin has broad potential applications in me...
Optimizing single and cascade microbial enzyme systems through site-directed mutagenesis for enhancing mycotoxin detoxification [0.03%]
通过定向进化优化单酶和级联酶系统以增强霉菌毒素解毒效能
Tosin Victor Adegoke,Sifan Lu,Ogedegbe Gloria Adegoke et al.
Tosin Victor Adegoke et al.
Mycotoxins, among the most extensively studied biological toxins, pose significant health risks to humans and animals, causing substantial economic losses in the agricultural sector. Numerous conventional enzymes isolated from microorganism...
Ying-Ying Xu,Sheng-Mei Zhou,Lu-Yan Wang et al.
Ying-Ying Xu et al.
The CRISPR/Cas9 system has emerged as a revolutionary tool for gene editing, widely used in the biomedical field due to its simplicity, efficiency, and cost-effectiveness. However, evidence suggests that CRISPR/Cas9 can induce off-target ef...
Precise control of transcriptional stoichiometry in bacteria: From mechanisms to synthetic biology applications [0.03%]
细菌转录比例的精准调控：从机制到合成生物学应用
Duodong Wang,Na Wang,Houhui Song et al.
Duodong Wang et al.
Bacteria exhibit remarkable precision in controlling the stoichiometry of protein subunits within metabolic pathways and macromolecular complexes-a requirement for optimal function and fitness. This review explores the RNA-level mechanisms ...
Cuifang Ye,Xiaoqian Li,Tao Liu et al.
Cuifang Ye et al.
Peroxisome engineering in yeast has emerged as a promising strategy for biomanufacturing, as it enables the compartmentalization of biosynthetic pathways and thus alleviates key bottlenecks in natural product biosynthesis. By sequestering s...
Advances in biosensors for microbial biosynthesis of amino acids and their derivatives [0.03%]
微生物合成氨基酸及其衍生物的生物传感器研究进展
Huina Dong,Tingting Li,Xinyi Ren et al.
Huina Dong et al.
Amino acids and their derivatives play pivotal roles across diverse fields including biotechnology, pharmaceuticals, agriculture, and industrial manufacturing. The development of high-throughput screening methods for strains producing amino...
Accelerated adaptive laboratory evolution: A tool for evolutionary biotechnology [0.03%]
加速的适应性实验室进化：一种进化生物技术工具
Luna Declerck,Florent Bouchon,Wouter Demeester et al.
Luna Declerck et al.
Adaptive laboratory evolution (ALE) is a powerful strategy for enhancing microbial traits by harnessing the principles of natural selection in controlled environments. It has enabled significant advances in microbial growth, stress toleranc...
Recent advances in microbial biosynthesis of L-cysteine and its derivative sulfur-containing antioxidants [0.03%]
近年来微生物法合成L半胱氨酸及其含硫抗氧化衍生物的研究进展
Hui Gao,JunJie Sun,Xian Zhang et al.
Hui Gao et al.
Sulfur is an essential element for life, and most microorganisms are actively involved in the global sulfur cycle through sulfur oxidation and sulfate reduction pathways. Among sulfur-containing metabolites, L-cysteine plays a central role ...
From IscB to Cas9: Engineering and advances in the next generation of miniature gene editing tools [0.03%]
从IscB到Cas9：下一代迷你基因组编辑工具的改造和进展
Changli Zhou,Sisi Zhu,Chengjian Luo et al.
Changli Zhou et al.
The CRISPR-Cas system, distinguished by its inherent modularity and broad programmability, has catalyzed a paradigm shift in genome engineering due to its unprecedented accuracy, specificity, and on-target efficiency, now serving as the cor...