GeneMiner2: Accurate and Automated Recovery of Genes From Genome Skimming Data [0.03%]
GeneMiner2：从基因组草图数据中准确而自动地恢复基因
Xinyi Yu,Zizhen Tang,Zhen Zhang et al.
Xinyi Yu et al.
With the growing accessibility of low cost genome skimming, large-scale recovery of target genes in non-model species has become feasible, providing strong data for phylogenomic and evolutionary studies. However, existing tools for data ass...
Damian Käch,Miguel Loera-Sánchez,Beat Reidy et al.
Damian Käch et al.
Permanent grasslands are predominantly composed of allogamous plant species that exhibit high levels of plant genetic diversity (PGD) within their populations. Grasslands with high PGD are more resilient to environmental stress and constitu...
Scoop That Poop: Optimising Faecal Sample Pre-Processing for Parasite Metabarcoding [0.03%]
优化寄生虫元条形码的粪便样品预处理方法
Madison E Patch,Graham B Goodman,Sara B Weinstein
Madison E Patch
Molecular techniques such as DNA metabarcoding are increasingly used to characterise parasite communities. However, relatively few studies have examined how sample processing methods influence detection rates. We used faecal samples collect...
Estimating Effective Number of Breeders Under the Heterozygote-Excess Equilibrium [0.03%]
过剩杂合平衡下的有效奠基者数量估计
Bing Yang,Dan Wang,Yujia Shen et al.
Bing Yang et al.
Estimating the effective number of breeders per reproductive cycle or cohort ( N b $$ {N}_b $$ ) can contribute to understanding the viability of wild populations. However, previous N b $$ {N}_b $$ estimators based on heterozygote-e...
Balancing Barcoding and Genomics: gDNA Quality in Insect Vouchers After HotSHOT DNA Extraction [0.03%]
基于HotSHOT基因组DNA提取的昆虫模式标本中gDNA质量与数量的优化研究
Vivian Feng,Caroline Høegh-Guldberg,Rudolf Meier et al.
Vivian Feng et al.
DNA extraction with an alkaline buffer system called 'HotSHOT' is widely used for barcoding because it is rapid, inexpensive, and voucher preserving, but it remains unclear whether sufficient genomic DNA (gDNA) remains in small vouchers for...
Estimating Organism Abundance Using Within-Sample Haplotype Frequencies of eDNA Data [0.03%]
基于环境DNA数据内样基因型频率估计生物量丰度数量模型研究
Pedro F P Brandão-Dias,Gledis Guri,Megan R Shaffer et al.
Pedro F P Brandão-Dias et al.
Environmental DNA (eDNA) provides powerful insights into species presence and community composition but remains limited in its capacity to infer species abundance or population structure. Here, we show that the deviation between within-samp...
Jinyang Liang,Julien Y Dutheil
Jinyang Liang
Asserting which allele is ancestral or derived, known as polarisation, is a prerequisite of many population and quantitative genetic methods. One important application is the inference of the unfolded site-frequency spectrum (uSFS). The mos...
Properties and Limitations of eDNA Substrates for Terrestrial Animal Monitoring [0.03%]
用于陆地动物监测的环境DNA基质的性质和局限性
Beilun Zhao,Tobias Andermann
Beilun Zhao
Environmental DNA (eDNA) constitutes a valuable tool for monitoring terrestrial animal diversity, but outcomes are affected by multiple factors. Among these factors, the choice of sampling substrate and method is especially important and mu...
From Permits to Samples: Addressing Key Challenges for High-Quality Reference Genome Generation in Europe [0.03%]
从许可证到样本：解决欧洲高质量参考基因组生成的关键挑战
Katja Reichel,Jaakko Pohjoismäki,Jonas J Astrin et al.
Katja Reichel et al.
High-quality reference genome assemblies have become essential for deepening our understanding of biodiversity, yet obtaining them for many species remains surprisingly challenging. Drawing on experiences from the European Reference Genome ...