Recovering Historical eDNA From Museum-Preserved Filter Feeders via Non-Destructive Metabarcoding [0.03%]
通过非破坏性元条形码技术从博物馆保存的滤食者中恢复历史性的环境DNA
Gert-Jan Jeunen,Sadie Mills,Marc Bailie et al.
Gert-Jan Jeunen et al.
Recent technical advances have significantly enhanced the value of museum specimens for molecular research, with metagenomic and metabarcoding approaches expanding further the utility of museum collections. However, given the finite number ...
GT-Seq Panel Development for Species Identification and Parentage Analysis of Closely Related Hybridising Scaphirhynchus Sturgeons [0.03%]
用于Scaphirhynchus 鳜鱼物种鉴定和亲缘关系分析的GT-Seq基因面板开发
Junman Huang,Richard Flamio Jr,Nathan R Campbell et al.
Junman Huang et al.
Hatchery supplementation is vital for conserving dwindling fish populations. Effective augmentation requires distinguishing hatchery-origin from wild individuals and accurately identifying species, particularly in systems where closely rela...
Accounting for Intra- and Intergenomic Sequence Variation in Reference Barcodes Improves eDNA Metabarcoding Biodiversity Assessment [0.03%]
考虑参考条形码中的基因组内和基因组间序列变异可改进环境DNA metabarcoding多样性评估
Luke J McCartin,Samuel A Vohsen,Annemarie L Wood et al.
Luke J McCartin et al.
Environmental DNA (eDNA) metabarcoding can rapidly characterise biodiversity, yet its accuracy and effectiveness are limited by incomplete DNA barcode reference databases. We evaluated how comprehensive reference databases that include sequ...
Comparative Multi-Marker Environmental DNA Metabarcoding of Marine Metazoan Communities: Water vs. Sediment [0.03%]
海洋多毛类群落海水与沉积物中eDNA metabarcoding比较研究:标志物间的差异分析
Alice Tagliabue,Giulia Furfaro,Alberto Pallavicini et al.
Alice Tagliabue et al.
This study investigates the metazoan biodiversity in the Southern Adriatic Sea using environmental DNA (eDNA) metabarcoding. Sediment and adjacent water samples were collected from three sites (one pristine, two impacted by human activities...
Comparative Study
Molecular ecology resources. 2026 Apr;26(3):e70126. DOI:10.1111/1755-0998.70126 2026
Limitations of Common Molecular Markers in Fungal Biodiversity Analysis and the Benefits of Their Synergistic Use [0.03%]
常用分子标记在真菌多样性分析中的局限性及其协同应用的优势
Vasilii Shapkin,Tomáš Zelenka,Tomáš Větrovský et al.
Vasilii Shapkin et al.
High-throughput sequencing of the Internal Transcribed Spacer (ITS) regions is the primary method for estimating fungal diversity from environmental DNA. However, reliance solely on ITS markers is complicated by its high variability in sequ...
Validating COI eDNA Metabarcoding Primers for Detection of Subterranean Fauna [0.03%]
用于检测地下生物的COI eDNA metabarcoding引物的有效性验证
Mieke van der Heyde,Michael Curran,Stephanie Floeckner et al.
Mieke van der Heyde et al.
Subterranean ecosystems host a diverse range of ancient fauna, but studying these ecosystems is challenging due to significant sampling difficulties. Environmental DNA (eDNA) metabarcoding offers a promising approach for monitoring subterra...
Development and Laboratory Validation of a Field-Deployable CRISPR-Cas12a eDNA Assay for Phylogeographic Lineage Detection in Arctic Char (Salvelinus alpinus) [0.03%]
用于北极Char(Salvelinus alpinus)的现场可部署CRISPR-Cas12a eDNA测定法的发展和实验室验证,以检测谱系地理种群
Darren J Walsh,Rosaleen Hynes,Weili Guo et al.
Darren J Walsh et al.
Environmental DNA (eDNA) tools are increasingly used for biodiversity monitoring, with most existing assays targeting species-level identification. However, the use of eDNA to resolve intraspecific genetic variation remains rare and methodo...
An Epigenetic Clock for Accurate Age Prediction in Atlantic Cod Populations for Improved Fisheries Management [0.03%]
用于准确预测大西洋鳕鱼种群年龄的表观遗传时钟以改善渔业管理
Dafni Anastasiadi,Yassine Kasmi,Christoph Stransky et al.
Dafni Anastasiadi et al.
Fisheries management relies on accurate stock assessments, which in turn depend on precise age information. Recent molecular tools called 'epigenetic clocks' harness age-related DNA methylation changes to build accurate and precise age-pred...
Comparative Genomics Provide Insights Into Karyotype Evolution in Vespertilionid Bats (Vespertilionidae, Chiroptera) [0.03%]
比较基因组学揭示了蝙蝠菊头蝠科物种的染色体进化规律
Linjing Lan,Xin Zhang,Jinjin Xie et al.
Linjing Lan et al.
Studies elucidating the molecular basis and evolutionary consequences of karyotypic changes in mammals remain scarce. Here, we investigate chromosomal evolution by focusing on two contrasting lineages within the family Vespertilionidae (Chi...
Disentangled Assembly Graphs Reveal Hidden Eukaryotic Diversity in eDNA Metagenomic Data [0.03%]
离散组装图揭示环境 DNA 元基因组数据中的隐藏真核生物多样性
Manon Mireille Geerts,Manuel Curto,Andrew J Alverson et al.
Manon Mireille Geerts et al.
Genome assembly graphs contain valuable yet frequently overlooked information that can enhance assembly completeness by revealing contig connectivity. Here, we demonstrate how leveraging these information-rich structures enables the discove...