Beyond Biodiversity: Incorporating Uncertainty Into Metabarcoding Data for Improved Inference of Ecological Relationships [0.03%]
超越生物多样性：将不确定性纳入元条形码数据中以改善生态关系的推断
Nastassia V Patin,Kathleen Pitz,Kimani Kimbrough et al.
Nastassia V Patin et al.
Metabarcoding sequence data from environmental DNA (eDNA) is rapidly expanding as a powerful method for biodiversity surveys. In order to interpret these data, tools are needed that account for the uncertainty associated with eDNA sampling,...
PopGenHelpR: An R Package to Streamline and Facilitate Informed Population Genomic Analyses and Visualization of Genetic Ancestry, Diversity and Differentiation [0.03%]
PopGenHelpR：一个用于促进和简化知情的群体基因组分析以及遗传祖先、多样性和差异化的可视化的R包
Keaka Farleigh,Mason O Murphy,Christopher Blair et al.
Keaka Farleigh et al.
Analysing large population genomic datasets requires an interdisciplinary skillset. Beyond a knowledge base in genetics and population biology, population genomic analyses involve computer science and statistics, representing a barrier for ...
Dietary RNA: Integration of RNA Data Offers a Potential Paradigm Shift for Molecular Dietary Analyses [0.03%]
饮食RNA：整合RNA数据为分子膳食分析提供了潜在的范式转变
Jordan P Cuff,James J N Kitson,Fredric M Windsor et al.
Jordan P Cuff et al.
Just over two decades ago, DNA-based dietary analysis promised to advance the resolution, sensitivity, and speed with which we could detect and identify trophic interactions. Since then, these approaches have generated a paradigm shift in o...
Optimising Zooplankton DNA Metabarcoding: Methodological Considerations for Large-Scale Monitoring [0.03%]
浮游动物DNA宏条形码优化：大规模监测方法论考虑因素
Elizaveta Ershova-Menze,Jon-Ivar Westgaard,Helene Hjellnes et al.
Elizaveta Ershova-Menze et al.
DNA metabarcoding is becoming an increasingly common approach in ecological monitoring of marine and freshwater planktonic communities, yet methodological choices along the metabarcoding workflow and data post-processing approaches remain h...
Evaluating the Molecular Potential and Interpretability of DNA in Historical Spirit Collection Media [0.03%]
评估历史精神收集介质中DNA的分子潜力和可解释性
Rachel L Tulloch,Jack Rojahn,Linda E Neaves et al.
Rachel L Tulloch et al.
Advancements in historical genomics increasingly leverage museum collections to study past ecosystems, species interactions and biodiversity. Formalin-fixed, ethanol-preserved specimens, once thought inaccessible to molecular analyses due t...
A Scalable and Cost-Effective In-Line Barcoding Strategy for Standardized 16S rRNA Gene Amplicon Sequencing: Performance Evaluation and Bias Assessment [0.03%]
一种可拓展且成本效益高的条形码方案用于标准化的16S rDNA基因扩增子测序：性能评估与偏差分析
Lisa Jourdain,Pierre Rossi,Aline Charpagne et al.
Lisa Jourdain et al.
In-line barcoding offers a streamlined and scalable alternative to two-step PCR library preparation for 16S rRNA gene amplicon sequencing, enabling cost-effective, high-throughput profiling of microbial communities. Here, we tested 136 and ...
On the Use of the Jackknife to Compare RXY Statistics in Conservation Genomics Research [0.03%]
刀切法在保护基因组学研究中比较RXY统计量的应用
Oliver Patrick Stuart
Oliver Patrick Stuart
Many useful quantities in conservation genomics are point estimates that summarise genetic variation at the population or species level. This presents a challenge to the application of null hypothesis testing frameworks, which require some ...
Hybrid Horizons: Screening Hybridisation Through Nuclear Environmental DNA [0.03%]
混合视野：通过核环境DNA筛查杂交现象
Daniel Zumel,Emilie Didaskalou,Tijana Vučić et al.
Daniel Zumel et al.
Effective monitoring of hybrid zones is essential for understanding evolutionary dynamics and mitigating species loss caused by human-mediated hybridisation. Conventional methods rely on sampling numerous individuals, which is costly, time-...
DIY: A Practical Field-to-Sequencer Workflow for Metabarcoding the Diet of Terrestrial Carnivore Species [0.03%]
DIY：陆生食肉动物饮食的代谢条形码从野外到序列器的实际工作流程
Francisca A S Virtuoso,Jos Boekhorst,Shasa van Ravenstein et al.
Francisca A S Virtuoso et al.
Metabarcoding of faecal samples is a powerful, non-invasive approach for investigating the feeding ecology of carnivores, revealing prey diversity and unexpected dietary components with greater resolution than traditional methods. However, ...
BINge: Multispecies Ortholog Clustering for Differential Gene Expression Analyses [0.03%]
BINge：差分基因表达分析的多物种直系同源物聚类
Zachary Stewart,Dimitri Perrin,Alexie Papanicolaou et al.
Zachary Stewart et al.
Differential gene expression (DGE) analysis enables researchers to investigate the link between gene expression and the phenotypic responses observed in organisms across time, experimental, or field conditions. Accurate quantification of ge...