pr2-Wormifier: A Bioinformatics Pipeline to Create Custom Reference Databases for Improved Metabarcoding of Marine Protists [0.03%]
改进海洋原生动物代谢条形码的生物信息学流程PR2-Wormifier：创建自定义参考数据库
Stefanie Knell,Juliane Romahn,Miklós Bálint
Stefanie Knell
Metabarcoding of environmental and ancient environmental DNA (eDNA and sedaDNA) is a powerful approach for studying and monitoring marine communities. However, its effectiveness is limited by the availability of comprehensive and well-curat...
Charlotte E Eriksson,Marcus Bianco,Joel Ruprecht et al.
Charlotte E Eriksson et al.
Noninvasive genetic sampling is widely used in ecology and conservation to identify predators and their diets but recovering individual-level information from consumed prey remains largely unexplored. We evaluated whether individual prey ca...
Wild Pedigree exploreR (wpeR): Streamlined Analysis and Visualization of Wild Pedigrees in Time and Space [0.03%]
野生谱系探索者（wpeR）：野生谱系在时间和空间中的分析和可视化的一站式服务
Gregor Simčič,Tomaž Skrbinšek
Gregor Simčič
Advances in non-invasive genetic sampling and long-term genetic monitoring programmes have enabled collection of large individual genotype datasets for many wildlife populations, often accompanied by rich field metadata that place the genot...
Integrating Megabarcoding and Metabarcoding to Unlock Diversity and Distribution Data Shortfalls in Dark Taxa [0.03%]
结合巨条形码和元条形码解锁暗淡类群的多样性与分布数据缺口
I Santos-Perdomo,A Salces-Castellano,M L Moraza et al.
I Santos-Perdomo et al.
Persistent biodiversity data shortfalls undermine our capacity to detect species, map their distributions and characterize their spatial genetic structure, limiting robust biogeographic analyses and the development of effective conservation...
BeeGees: A High-Throughput Protein-Coding DNA Barcode Recovery Pipeline Tailored for Genome Skims of Museum Specimens [0.03%]
BeeGees：一种针对博物馆标本基因组概要的高通量蛋白质编码DNA条形码回收流水线
Daniel A J Parsons,Rutger A Vos,Benjamin W Price
Daniel A J Parsons
Natural history collections are unparalleled archives of global biodiversity, yet most specimens remain molecularly uncharacterised due to the technical challenges of historical DNA (hDNA), including degradation, low endogenous content and ...
De Novo Genome Assemblies of Four Rainbow Trout Genetic Lines Reveal Structural Variants in Pursuit of a Pangenome Reference [0.03%]
从四种虹彩 trout 基因组中 de novo 组装出的四个虹鳟遗传系基因组揭示了结构变异以追求泛基因组参考序列
Ali Ali,Geoffrey C Waldbieser,Ramey C Youngblood et al.
Ali Ali et al.
Rainbow trout (Oncorhynchus mykiss) exhibit extensive genomic diversity shaped by domestication, life history and geographic origin. To advance the development of a comprehensive pangenome reference, we present new de novo genome assemblies...
Fragmentation of Long Reads Enables Reliable Mitogenome Assembly From Whole-Genome Amplification Data With Pervasive Palindromic Reads [0.03%]
长读段碎片化使拼接全基因组扩增数据中的线粒体基因组序列成为可能，具有广泛回文片段读取的特点
Matteo Vecchi,Bartłomiej Surmacz,Ingemar K Jönsson et al.
Matteo Vecchi et al.
Whole genome amplification (WGA), and in particular multiple displacement amplification (MDA), has become a key technique for genomic sequencing of microscopic organisms, yet it introduces artefacts such as palindromic (inverted chimeric) r...
Treeline Provides a Unified Strategy for Optimising Phylogenetic Trees Under Alternative Criteria [0.03%]
用于在替代标准下优化系统发生树的统一策略
Erik S Wright
Erik S Wright
The choice of optimality criterion is a key consideration in phylogenetic studies. Recent work challenges the notion that more computationally demanding optimisation objectives result in better phylogenetic trees. This finding underscores t...
Using Metabarcoding Techniques to Map Multiple Dung Beetle-Vertebrate iDNA Networks in a Southeast Asian Forest [0.03%]
基于东南亚森林中多类粪甲虫-脊椎动物的iDNA网络运用 metabarcoding 技术制图
Xin Rui Ong,Rosie Drinkwater,Samuel K S Cheong et al.
Xin Rui Ong et al.
Given the high levels of endemism, diversity, and increasing anthropogenic impacts in tropical regions, studies on species interactions and ecological networks are urgently needed to understand community responses to environmental change. I...
A Roadmap for Using Hybridisation Capture-Based Target Enrichment of Ancient Environmental DNA in Palaeoecology [0.03%]
基于古代环境DNA的杂交捕获靶点富集在古生态学中的路线图
Nicole R Foster,Luke E Holman,Linda Armbrecht et al.
Nicole R Foster et al.
Recovering ancient DNA from environmental samples is transforming the way we understand historical ecosystems. While high-throughput sequencing of the total DNA in environmental samples (shotgun metagenomic sequencing) reveals the taxonomic...