The Effects of Post-Divergence Gene Flow on Simultaneous Divergence Time Testing [0.03%]
分化后的基因流对同时发散时间测试的影响
Michael A Tofflemire,Kevin J Burns,Jeet Sukumaran
Michael A Tofflemire
Estimating simultaneous divergence across multiple taxonomic groups from genetic data is a common approach in phylogeographical studies, providing insight into the historical and evolutionary factors shaping species diversification. However...
The Pan-Genome of 1107 Spotted Sea Bass Accessions Reveals Gene Evolution Patterns During Domestication Selection [0.03%]
基于月鳢的泛基因组揭示人工驯化选择下的鱼类基因演化模式
Chong Zhang,Cong Liu,Jinxi Hua et al.
Chong Zhang et al.
Spotted sea bass (Lateolabrax maculatus) is an ecologically and economically important species that supports a large-scale aquaculture industry in China. However, most farmed populations have not undergone systematic breeding programs and a...
Comparing Accuracy and Biases of DNA Metabarcoding, Hybridization Capture, and Metagenomic Sequencing for Quantifying Herbivore Diets [0.03%]
比较DNA metabarcoding、杂交捕获和宏基因组测序在量化食草动物饮食中的准确性与偏差
Charlotte E Eriksson,Lisa Shipley,Darren A Clark et al.
Charlotte E Eriksson et al.
DNA metabarcoding using relative read abundance (RRA) is commonly applied to estimate herbivore diet composition, yet its quantitative accuracy remains uncertain. We assessed taxonomic resolution and quantitative performance of RRA from DNA...
Comparative Study
Molecular ecology resources. 2026 Jul;26(5):e70175. DOI:10.1111/1755-0998.70175 2026
K L Vinay,Naman Goyal,Ashwin Warudkar et al.
K L Vinay et al.
Tropical regions are biodiversity-rich, yet remain underrepresented in the availability of genomic resources, as is evident in the Western Ghats of India, a biodiversity hotspot with high endemism. Here, we present high-quality, de novo gen...
Phylogenetic Authentication of Amplicon Sequence Variants in Single-Specimen Metabarcoding of Tropical Insects [0.03%]
基于单一标本的热带昆虫宏条形码序列变体的系统发育认证
Sarawut Ounjai,Huaxi Liu,Zichen Zhou et al.
Sarawut Ounjai et al.
High-throughput sequencing (HTS) allows large-scale DNA barcoding of individually tagged specimens ('megabarcoding'), but deep amplicon sequencing produces a mixture of authentic mitochondrial sequences together with nuclear pseudogenes (NU...
Single-Cell Transcriptomics and Development of Gametocyte-Specific Molecular Markers for Avian Malaria Parasites [0.03%]
单细胞转录组和鸟疟原虫配子体特异性分子标记物的开发
A Berthomieu,I Rizos,J W Bagnoli et al.
A Berthomieu et al.
Avian malaria, caused by Plasmodium parasites, poses a significant threat to bird populations worldwide, particularly in vulnerable island ecosystems. Yet, progress in understanding avian malaria transmission dynamics has been hampered by t...
From Gene Copies to Cell Numbers: Advancing Quantitative Approaches in Protistan Ecology Using Digital PCR [0.03%]
从基因拷贝到细胞数量：使用数字PCR推进原生动物生态学的定量方法
Megan Gross,Ulrike Koll,Bettina Sonntag et al.
Megan Gross et al.
Quantifying abundances of unicellular eukaryotes (protists) remains a central challenge in microbial ecology, as methodological differences can strongly influence abundance estimates and ecological interpretation. Although molecular tools h...
EasyCen: A Lightweight Framework for Centromere Localisation and Repeat-Organisation Profiling in Telomere-to-Telomere Genomes [0.03%]
易居中心框架：端粒到端粒基因组中着丝粒定位和重复序列构象分析的轻量级框架
Yunyun Lv,Yanping Li,Jia Li et al.
Yunyun Lv et al.
Accurate identification of centromeres in telomere-to-telomere (T2T) genomes remains difficult due to the rapid evolution of centromeric repeats and their lack of conserved sequence features. In this study, we present EasyCen, a lightweight...
Chandika Rg,Caitlin N Ott-Conn,Peter T Euclide et al.
Chandika Rg et al.
Genotyping-in-thousands by sequencing (GT-seq) panels are powerful tools in ecological, evolutionary and conservation genomics, yet the optimization process critical for robust and reproducible genotyping remains poorly formalized. Here, we...
Comparison of Environmental DNA and Bulk DNA Metabarcoding for Assessing Terrestrial Arthropod Diversity Across Three Habitat Types on Guam [0.03%]
比较环境DNA和总DNA metabarcoding评估关岛三种生境类型的陆地节肢动物多样性的方法
Pritam Banerjee,Samantha Al-Bayer,Jerilyn Calaor et al.
Pritam Banerjee et al.
DNA-based methods offer a rapid and cost-effective way for detecting species occurrence and monitoring biodiversity; among them, bulk DNA metabarcoding is well-established, and recently developed environmental DNA (eDNA)-based methods offer...
Comparative Study
Molecular ecology resources. 2026 Jul;26(5):e70172. DOI:10.1111/1755-0998.70172 2026