Benchmarking Full-Length ITS Metabarcoding Across Illumina 2 × 500, PacBio, and Oxford Nanopore Sequencing Using Mock and Soil Communities [0.03%]
基于模拟和土壤群落利用Illumina 2×500、PacBio和Oxford Nanopore测序对全长ITS元条形码进行基准测试
Leho Tedersoo,Marko Prous,Meirong Chen et al.
Leho Tedersoo et al.
Metabarcoding is a powerful tool for biodiversity comparisons, where standard-size DNA barcodes (> 500 bases) offer better taxonomic resolution than shorter ones. Still, the choice of sequencing platforms and bioinformatics pipelines may st...
FishChip: Development of a High-Throughput qPCR Assay for Freshwater Fish eDNA Quantification and Its Comparison With Droplet Digital PCR [0.03%]
鱼芯片：淡水鱼类eDNA定量的高通量qPCR测定法及其与微滴式数字pcr比较研究
Shoutao Cheng,Jiasheng Zhang,Zhuming Liang et al.
Shoutao Cheng et al.
Quantitative monitoring of fish using environmental DNA (eDNA) typically relies on quantitative real-time PCR (qPCR) and droplet digital PCR (ddPCR), yet both methods are constrained in throughput, making it difficult to meet the demands of...
Comparative Study
Molecular ecology resources. 2026 Aug;26(6):e70187. DOI:10.1111/1755-0998.70187 2026
Improving Malaria Parasite Gene Flow Inference Under Sparse Spatial Sampling [0.03%]
稀疏抽样下的疟疾基因流动推断改进
Yao Li,Bing Guo,Timothy D O&#x;Connor et al.
Yao Li et al.
Estimating malaria parasite migration is crucial for informing elimination strategies, particularly by identifying regions with higher parasite migration that may serve as transmission sources and intervention targets. Methods that infer sp...
Umbilical Cord: A Non-Invasive DNA Source for Sea Turtle Genomics [0.03%]
用于海龟基因组学的非侵入性DNA来源——胎盘脐带丝组织样本
Gisela Marín-Capuz,Elena Abella,Marta Pascual et al.
Gisela Marín-Capuz et al.
High-throughput sequencing has revolutionised conservation genomics by enabling genome-wide information even in non-model species. However, obtaining non-invasive samples for genomics remains challenging in species like sea turtles, which s...
Operationalising Environmental DNA (eDNA) Detection of Major Malaria Vector Species in Ghana [0.03%]
在加纳将环境DNA(eDNA)检测主要疟疾媒介物种付诸实施
Mojca Kristan,Yaw A Afrane,Sophie Moss et al.
Mojca Kristan et al.
The global landscape of vector-borne diseases (VBDs) is shifting, driven by climate change, urbanisation and widespread habitat modification, requiring novel approaches to surveillance, prevention and control. To improve our capacity to res...
Genomic Data Describe Population Structure but Struggle to Estimate Directed Connectivity Networks [0.03%]
基因组数据可描述种群结构但难以估计有向连接网络
Camille Sant,Didier Forcioli,Cécile Fauvelot et al.
Camille Sant et al.
The movement of individuals between fragmented populations, known as population connectivity, plays a crucial role in population persistence, genetic structure and, ultimately, the definition of a species distribution range. Advances in mol...
Enrichment Probe Sets Combining Universal and Lineage-Specific Targets Help Resolve Recalcitrant Lineages [0.03%]
结合通用和谱系特异性靶标的富集探针集合有助于解析难处理的谱系
Irene Villa-Machío,Irene Masa-Iranzo,Nicolai M Nürk et al.
Irene Villa-Machío et al.
The combination of target capture sequencing (TCS) with low-coverage whole genome sequencing (lcWGS), an approach known as Hyb-Seq, has allowed the integration of natural history collections into the genomics revolution, transforming biodiv...
Unified Multi-Caller Ensemble (UME) Generates an Unbiased Maize Haplotype Map for Variable Coverage Whole Genome Data [0.03%]
统一的多调用器集成（UME）生成用于变量覆盖率全基因组数据的无偏倚的玉米单倍型图谱
Miguel Vallebueno-Estrada,Kelly Swarts
Miguel Vallebueno-Estrada
We present a novel diversity-focused haplotype map (HapMap) that characterizes over 64.5 million maize (Zea mays ssp. mays) single nucleotide polymorphisms (SNPs) genotyped across 818 individuals from diverse backgrounds. This HapMap aims t...
Imputation in the Wild: Genome-Wide Robustness and Fine-Scale Limitations of Low-Coverage Genomes in Endangered Species [0.03%]
野生环境中的数据填补：濒危物种低覆盖率基因组的全基因组稳健性和细尺度限制性
Lucía Mayor-Fidalgo,Enrico Bazzicalupo,Laia Pérez-Sorribes et al.
Lucía Mayor-Fidalgo et al.
Low-coverage whole-genome sequencing (lcWGS) combined with genotype imputation is increasingly being used to generate large genomic datasets at reduced cost, offering a promising alternative for conservation genomics. Here, we use the Iberi...