Endogenous CRISPR-assisted microhomology-mediated end joining enables rapid genome editing in Zymomonas mobilis [0.03%]
内源性CRISPR介导的同源重组使得Zymomonas mobilis的基因组编辑成为可能
Xin Sui,Xiaojie Wang,Tao Liu et al.
Xin Sui et al.
Background: Zymomonas mobilis is a natural ethanologen with many desirable characteristics, making it an ideal platform for future biorefineries. Recently, an endogenous CRISPR-based genome editing tool has been developed...
CRISPR/Cas9-mediated genome editing directed by a 5S rRNA-tRNAGly hybrid promoter in the thermophilic filamentous fungus Humicola insolens [0.03%]
热嗜酸子囊菌Humicola insolens中由5S rRNA-tRNAGly杂交启动子指导的CRISPR / Cas9介导的基因组编辑技术研究
Chao Fan,Wei Zhang,Xiaoyun Su et al.
Chao Fan et al.
Background: Humicola insolens is a filamentous fungus with high potential of producing neutral and heat- and alkali-resistant cellulase. However, the genetic engineering tools, particularly the genome-editing tool, are sc...
Recent advances in understanding the effects of lignin structural characteristics on enzymatic hydrolysis [0.03%]
对木质素结构特征对酶解效应影响的理解 recent advances 在理解木质素结构特性对酶水解作用的影响方面的最新进展
Yufeng Yuan,Bo Jiang,Hui Chen et al.
Yufeng Yuan et al.
Enzymatic hydrolysis of lignocellulose for bioethanol production shows a great potential to remit the rapid consumption of fossil fuels, given the fact that lignocellulose feedstocks are abundant, cost-efficient, and renewable. Lignin resul...
Improvement of thermostability and catalytic efficiency of glucoamylase from Talaromyces leycettanus JCM12802 via site-directed mutagenesis to enhance industrial saccharification applications [0.03%]
通过对Talaromyces leycettanus JCM12802麦芽糖酶定点突变提高其热稳定性和催化效率以增强工业糖化应用
Lige Tong,Jie Zheng,Xiao Wang et al.
Lige Tong et al.
Background: Glucoamylase is an important industrial enzyme in the saccharification of starch into glucose. However, its poor thermostability and low catalytic efficiency limit its industrial saccharification applications....
Citrulline deiminase pathway provides ATP and boosts growth of Clostridium carboxidivorans P7 [0.03%]
瓜氨酸脱水酶途径为P7型产羧基粘胞菌提供ATP并促进其生长
Xiangfei Li,Rumeng Han,Teng Bao et al.
Xiangfei Li et al.
Background: Clostridium carboxidivorans P7 is capable of producing ethanol and butanol from inexpensive and non-food feedstock, such as syngas. Achieving improved ethanol and butanol production in the strain for industria...
Comprehensive evaluation for the one-pot biosynthesis of butyl acetate by using microbial mono- and co-cultures [0.03%]
利用微生物单菌和共培养体系一步生物合成正丁酸乙酯的综合评价研究
Yang Lv,Yujia Jiang,Jiasheng Lu et al.
Yang Lv et al.
Background: Butyl acetate has shown wide applications in food, cosmetics, medicine, and biofuel sectors. These short-chain fatty acid esters can be produced by either chemical or biological synthetic process with correspo...
RNA sequencing reveals metabolic and regulatory changes leading to more robust fermentation performance during short-term adaptation of Saccharomyces cerevisiae to lignocellulosic inhibitors [0.03%]
RNA测序揭示了酿酒酵母在短期适应木质纤维素抑制剂过程中导致发酵性能更强的代谢和调节变化
Marlous van Dijk,Peter Rugbjerg,Yvonne Nygård et al.
Marlous van Dijk et al.
Background: The limited tolerance of Saccharomyces cerevisiae to inhibitors is a major challenge in second-generation bioethanol production, and our understanding of the molecular mechanisms providing tolerance to inhibit...
Glutamine involvement in nitrogen regulation of cellulase production in fungi [0.03%]
谷氨酰胺在真菌中参与氮调节纤维素酶的生产
Ai-Ping Pang,Funing Zhang,Xin Hu et al.
Ai-Ping Pang et al.
Background: Cellulase synthesized by fungi can environment-friendly and sustainably degrades cellulose to fermentable sugars for producing cellulosic biofuels, biobased medicine and fine chemicals. Great efforts have been...
Hsien-Lin Liu,Christine H-T Wang,En-Pei Isabel Chiang et al.
Hsien-Lin Liu et al.
Background: Isobutanol is considered a potential biofuel, thanks to its high-energy content and octane value, limited water solubility, and compatibility with gasoline. As its biosynthesis pathway is known, a microorganis...
Engineering Escherichia coli for highly efficient production of lacto-N-triose II from N-acetylglucosamine, the monomer of chitin [0.03%]
工程改造大肠杆菌高效地从壳多糖单体N-乙酰氨基葡萄糖生产乳糖-N-三糖II
Duoduo Hu,Hao Wu,Yingying Zhu et al.
Duoduo Hu et al.
Background: Lacto-N-triose II (LNT II), an important backbone for the synthesis of different human milk oligosaccharides, such as lacto-N-neotetraose and lacto-N-tetraose, has recently received significant attention. The ...