Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution [0.03%]
大肠杆菌β-葡萄糖苷酶D2-BGL在毕赤酵母中生产产率和糖化效率的提升方向演变
Mu-Rong Kao,Su-May Yu,Tuan-H Ua David Ho
Mu-Rong Kao
Background: β-Glucosidases are essential for cellulose hydrolysis by catalyzing the final cellulolytic degradation of cello-oligomers and cellobiose to glucose. D2-BGL is a fungal glycoside hydrolase family 3 (GH3) β-gl...
Revealing two important tryptophan residues with completely different roles in a dye-decolorizing peroxidase from Irpex lacteus F17 [0.03%]
白腐菌Irpex lacteus F17一株色素脱色过氧化物酶中两个色氨酸残基的不同作用机制研究
Liuqing Li,Tao Wang,Taohua Chen et al.
Liuqing Li et al.
Background: Dye-decolorizing peroxidases (DyPs) represent a novel family of heme peroxidases that use H2O2 as the final electron acceptor to catalyze the oxidation of various organic compounds. A DyP from Irpex lacteus F1...
Evaluation of acidogenesis products' effect on biogas production performed with metagenomics and isotopic approaches [0.03%]
基于元基因组和同位素方法评价酸化产物对生物气产甲烷过程的影响
Anna Detman,Michał Bucha,Laura Treu et al.
Anna Detman et al.
Background: During the acetogenic step of anaerobic digestion, the products of acidogenesis are oxidized to substrates for methanogenesis: hydrogen, carbon dioxide and acetate. Acetogenesis and methanogenesis are highly i...
Oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysate [0.03%]
oleaginous酵母对木质纤维素水解物中存在的碳源的响应不同
Jule Brandenburg,Johanna Blomqvist,Volha Shapaval et al.
Jule Brandenburg et al.
Background: Microbial oils, generated from lignocellulosic material, have great potential as renewable and sustainable alternatives to fossil-based fuels and chemicals. By unravelling the diversity of lipid accumulation p...
Precise high-throughput online near-infrared spectroscopy assay to determine key cell wall features associated with sugarcane bagasse digestibility [0.03%]
用于测定与甘蔗渣消化性相关的细胞壁关键特征的高通量在线近红外光谱测定法
Xinru Li,Fumin Ma,Chengping Liang et al.
Xinru Li et al.
Background: Sugarcane is one of the most crucial energy crops that produces high yields of sugar and lignocellulose. The cellulose crystallinity index (CrI) and lignin are the two kinds of key cell wall features that acco...
Novel molecular biological tools for the efficient expression of fungal lytic polysaccharide monooxygenases in Pichia pastoris [0.03%]
用于在毕赤酵母中高效表达丝状真菌聚糖单加氧酶的新型分子生物学工具
Lukas Rieder,Katharina Ebner,Anton Glieder et al.
Lukas Rieder et al.
Background: Lytic polysaccharide monooxygenases (LPMOs) are attracting large attention due their ability to degrade recalcitrant polysaccharides in biomass conversion and to perform powerful redox chemistry. ...
Quantitative proteomic comparison of salt stress in Chlamydomonas reinhardtii and the snow alga Chlamydomonas nivalis reveals mechanisms for salt-triggered fatty acid accumulation via reallocation of carbon resources [0.03%]
采用定量蛋白质组学比较盐藻和雪藻在盐胁迫下的脂肪酸积累机制及碳资源的再分配
E Hounslow,C A Evans,J Pandhal et al.
E Hounslow et al.
Background: Chlamydomonas reinhardtii is a model green alga strain for molecular studies; its fully sequenced genome has enabled omic-based analyses that have been applied to better understand its metabolic responses to s...
From simple and specific zymographic detections to the annotation of a fungus Daldinia caldariorum D263 that encodes a wide range of highly bioactive cellulolytic enzymes [0.03%]
从简单的斑点酶活测定到报道一株活性广泛且高效的纤维素降解真菌Daldinia caldariorum D263孤儿基因簇
Meng-Chun Lin,Hsion-Wen Kuo,Mu-Rong Kao et al.
Meng-Chun Lin et al.
Background: Lignocellulolytic enzymes are essential for agricultural waste disposal and production of renewable bioenergy. Many commercialized cellulase mixtures have been developed, mostly from saprophytic or endophytic ...
The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis [0.03%]
产虾青素微藻裂壶藻索氏褐变 strain 的潜力：从生产到脂质代谢和类胡萝卜素生物合成研究的新兴模型
Yu Zhang,Ying Ye,Fan Bai et al.
Yu Zhang et al.
The algal lipids-based biodiesel, albeit having advantages over plant oils, still remains high in the production cost. Co-production of value-added products with lipids has the potential to add benefits and is thus believed to be a promisin...
Synergistic mechanism of GH11 xylanases with different action modes from Aspergillus niger An76 [0.03%]
来源于Aspergillus niger An76的具有不同作用模式的GH11木聚糖酶之间的协同机制研究
Shu Zhang,Sha Zhao,Weihao Shang et al.
Shu Zhang et al.
Background: Xylan is the most abundant hemicellulose polysaccharide in nature, which can be converted into high value-added products. However, its recalcitrance to breakdown requires the synergistic action of multiple enz...