Engineering prime editors in Salvia miltiorrhiza for precise genome modification [0.03%]
药用植物丹参精准基因组编辑工程化prime editor系统的创建及应用研究
Qi Yao,Yi Ye,Muyao Yu et al.
Qi Yao et al.
An optimized prime editing system in the model medicinal plant Danshen (Salvia miltiorrhiza) was developed, enabling base transversions, complex insertions and base substitutions. Precise editing of key genes generated germplasm with elevat...
Base editing in rice using nuclease-deactivated CRISPR/Cas-SF01 [0.03%]
基于CRISPR/Cas-SF01核酸内切酶失活系统的水稻碱基编辑器
Meng Jia,Yangqing Xie,Chuang Wei et al.
Meng Jia et al.
Adenine and cytosine base editing using dCas-SF01 and the 35S-CmYLCV-U6 composite promoter successfully introduced targeted base substitutions at multiple loci in rice, with average editing efficiency of 33.3%. Using the protospacer adjacen...
SIB1-SEC23A undergo ER to chloroplast relocalization to mediate immunity in Arabidopsis thaliana [0.03%]
拟南芥SIB1-SEC23A介导的免疫反应中的内质网至叶绿体重定位现象
Jialin Peng,Huan Zhong,Jianan Zhang et al.
Jialin Peng et al.
Inter-organellar communication has emerged as a critical factor in maintaining cellular homeostasis under stress conditions. Chloroplasts function not only as central organelles for energy production but are also increasingly recognized as ...
From recognition to proteolytic control: NLRs and metacaspases in plant antiviral immunity [0.03%]
从识别至蛋白酶控制：NLR和半胱氨酸天冬氨酸蛋白酶（metacaspase）在植物抗病毒免疫中的作用
Lin Liang,Yuhang Jiang,Mengrong Wang et al.
Lin Liang et al.
Plant immunity requires not only intracellular recognition of pathogen invasion but also downstream execution mechanisms that directly restrict pathogen proliferation and spread. Nucleotide-binding leucine-rich repeat receptors (NLRs) funct...
OmicsCanvas: A multi-omics platform for integration and visualization of epigenetic regulation [0.03%]
OmicsCanvas：一种表观遗传调控的多组学整合及可视化平台
Zeyu Zhang,Zuoling Ma,Tian Hua et al.
Zeyu Zhang et al.
OmicsCanvas integrates diverse epigenomic and transcriptomic data into a unified framework, enabling users to visualize complex multi-omics patterns, generate publication-ready figures, and uncover regulatory relationships. This tool makes ...
Pangenome analysis reveals the genetic mechanism underlying high-altitude adaptation in Qinghai-Xizang (Tibet) Plateau Rhododendron [0.03%]
泛基因组分析揭示青藏高原杜鹃花高位居适应的遗传机制
Haoyang Zhou,Zhongping Xu,Fanhuang Zeng et al.
Haoyang Zhou et al.
Rhododendron, a globally important group of alpine flowering plants, provides an exceptional system for investigating ecological adaptation and stress resistance owing to its high-altitude specialization. Using 18 Rhododendron species, a gr...
Louise Vilain,Marie-Laure Fauconnier,Manon Genva
Louise Vilain
From seed dormancy to pathogen attacks and senescence, many events in a plant's life are critically controlled by low-molecular-weight signaling molecules, called plant hormones. These master regulators include abscisic acid, auxins, brassi...
Engineering the efficient Exo-Cas12i2 for MITE manipulation in rice [0.03%]
工程化高效的水稻小型回文重复序列元件操纵工具Exo-Cas12i2
Nan Chai,Wuge Liu,Kangli Sun et al.
Nan Chai et al.
Exo-Cas12i2 v1, a fusion of the 5' exonucleases T5E and PapE, facilitates editing of TA-rich regions and mediates deletions of large genomic fragments. Exo-Cas12i2 v1-driven MITE manipulation enables precise regulation of genes involved in ...
The mechanism of newly differentiated leaves of kale turning white in late autumn [0.03%]
晚秋时节羽衣甘蓝新生叶片变白的机制研究
Zhichao Gong,Yupeng He,Xinyuan Li et al.
Zhichao Gong et al.
Newly differentiated leaves of albino ornamental kale turn white as exposed to low temperatures, producing a distinctive and commercially desirable unique color pattern. Here, we show that this low-temperature-induced albinism is caused by ...
Enhancing CRISPR-Cas12a base editing in plants with LbCas12a variants and introns [0.03%]
利用LbCas12a变异体和内含子在植物中增强CRISPR-Cas12a碱基编辑
Yanhao Cheng,Gen Li,Man Zhou et al.
Yanhao Cheng et al.
Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools for precise genome editing in plants. Conventionally, such base editors are built upon the CRISPR-Cas9 systems where Cas9 nickases are used. To expand the base ...