Large-scale analysis of protein-protein interactions using cellulose-bound peptide arrays [0.03%]
基于纤维素固定化肽点阵的大规模蛋白质-蛋白质相互作用分析
Ulrike Beutling,Kai Städing,Theresia Stradal et al.
Ulrike Beutling et al.
Peptide arrays for screening large numbers of peptide fragments and probing with large numbers of samples is discussed.
Peter R Shewry,Huw D Jones,Nigel G Halford
Peter R Shewry
Transgenesis is an important adjunct to classical plant breeding, in that it allows the targeted manipulation of specific characters using genes from a range of sources. The current status of crop transformation is reviewed, including metho...
Filamentous fungi for production of food additives and processing aids [0.03%]
丝状真菌在生产食品添加剂和加工助剂中的应用
David B Archer,Ian F Connerton,Donald A MacKenzie
David B Archer
Filamentous fungi are metabolically versatile organisms with a very wide distribution in nature. They exist in association with other species, e.g. as lichens or mycorrhiza, as pathogens of animals and plants or as free-living species. Many...
Identification of protein-protein interactions by mass spectrometry coupled techniques [0.03%]
质谱联用技术鉴定蛋白质相互作用
Mohamed Abu-Farha,Fred Elisma,Daniel Figeys
Mohamed Abu-Farha
The use of mass spectrometry in protein identification has revolutionized the field of proteomics. Coupled to various affinity purification techniques, mass spectrometry is used to identify protein-protein interactions. This chapter looks a...
Catherine S Chan,Tara M L Winstone,Raymond J Turner
Catherine S Chan
The identification of protein interaction partners can often elucidate the function of the protein under investigation based on the "guilty by association" concept. Furthermore, the binding event between two proteins can be used as a functi...
Hongtao Guan,Endre Kiss-Toth
Hongtao Guan
One of the key challenges of biology in the post-genomic era is to assign function to the many genes revealed by large-scale sequencing programmes, since only a small fraction of gene function can be directly inferred from the coding sequen...
Sylvain Pitre,Md Alamgir,James R Green et al.
Sylvain Pitre et al.
Protein-protein interactions (PPIs) play a critical role in many cellular functions. A number of experimental techniques have been applied to discover PPIs; however, these techniques are expensive in terms of time, money, and expertise. The...
Shawn Martin,W Michael Brown,Jean-Loup Faulon
Shawn Martin
There is a wide variety of experimental methods for the identification of protein interactions. This variety has in turn spurred the development of numerous different computational approaches for modeling and predicting protein interactions...