Chandranandani Negi,Neeraj Kumar Vasistha,Dharmendra Singh et al.
Chandranandani Negi et al.
Plant gene editing has become an important molecular tool to revolutionize modern breeding of crops. Over the past years, remarkable advancement has been made in developing robust and efficient editing methods for plants. Despite a variety ...
BSA-PEI Nanoparticle Mediated Efficient Delivery of CRISPR/Cas9 into MDA-MB-231 Cells [0.03%]
BSA-PEI纳米颗粒介导的CRISPR/Cas9在MDA-MB-231细胞中的有效递送
Hossein Rahimi,Kasra Arbabi Zaboli,Jose Thekkiniath et al.
Hossein Rahimi et al.
The discovery of bacterial-derived Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has revolutionized genome engineering and gene therapy due to its wide range of applications. On...
Further Characterization of MUAS35SCP and FUAS35SCP Recombinant Promoters and Their Implication in Translational Research [0.03%]
MUAS35SCP和FUAS35SCP重组启动子的进一步表征及其在转化研究中的应用价值
Lini Sethi,Tsheten Sherpa,Khushbu Kumari et al.
Lini Sethi et al.
Recombinant promoters are of high value in translational research. Earlier, we developed two recombinant promoters, namely MUAS35SCP and FUAS35SCP, and their transcriptional activities were found to be stronger than that of the most widely ...
Let-7c-5p Restrains Cell Growth and Induces Apoptosis of Lung Adenocarcinoma Cells via Targeting ESPL1 [0.03%]
let-7c-5p通过靶向ESPL1抑制肺癌腺癌细胞增殖并诱导其凋亡
Xiang Liu,Wei Zeng,Dayang Zheng et al.
Xiang Liu et al.
Lung adenocarcinoma (LUAD) is a predominant malignancy, and its high mortality prompts us to incessantly probe the relevant targeted treatment. This work intended to study the molecular mechanism of ESPL1 in LUAD. Bioinformatics analysis wa...
Impact of Lysosomal Fraction on Melanin Synthesis Inhibition in a Zebrafish Melanogenesis Phenotype Model [0.03%]
溶酶体组分对斑马鱼黑色素合成抑制的效应研究模型
Gyeongchan Jeon,Yang-Hoon Kim,Jiho Min
Gyeongchan Jeon
To develop whitening cosmetic materials, we conducted a study on lysosomes that decisively contribute to the decomposition of melanin during autophagy in keratinocytes. In this study, we found that the lysosomal fraction inhibits melanin sy...
LncRNA VPS9D1-AS1 Sponging miR-520a-5p Contributes to the Development of Uterine Corpus Endometrial Carcinoma by Enhancing BIRC5 Expression [0.03%]
长链非编码RNA VPS9D1-AS1通过海绵吸附miR-520a-5p促进BIRC5表达从而参与子宫体子宫内膜癌发生发展的作用机制研究
Lu Chen,Meng Shen
Lu Chen
The pattern of VPS9D1-AS1 expression and its effects on uterine corpus endometrial carcinoma (UCEC) remained unclear. VPS9D1-AS1, miR-520a-5p, and BIRC5 mRNA levels were quantified by qRT-PCR. Bax, Bcl-2, N-cadherin, E-cadherin, and BIRC5 p...
Mi-Young Heo,Bit-Na Kim,Jaewoong Lee et al.
Mi-Young Heo et al.
In this study, we were investigated the effect of lysosomal extracts (named as lysosomal enzymes) on extending the vase life of cut flowers. The results confirmed that senescence of cut freesia treated with lysosomal enzymes delayed. Also, ...
Structural Organization of Human Full-Length PAR3 and the aPKC-PAR6 Complex [0.03%]
全长PAR3的结构组织及aPKC-PAR6复合物的结构
Le T M Le,Srdja Drakulic,Jens R Nyengaard et al.
Le T M Le et al.
The tripartite partition defect (PAR) polarity complex, which includes the proteins PAR3, atypical protein kinase C (aPKC), and PAR6, is a major regulator of cellular polarity. It is highly conserved and expressed in various tissues. Its la...
Evaluation of Methylotrophic Yeast Ogataea thermomethanolica TBRC 656 as a Heterologous Host for Production of an Animal Vaccine Candidate [0.03%]
热甲醇利用毕赤酵母 Ogataea thermomethanolica TBRC 656 高效表达动物疫苗候选蛋白的价值评估
Benjamas Liwnaree,Katanchalee Muensaen,Jaraspim Narkpuk et al.
Benjamas Liwnaree et al.
Multiple yeast strains have been developed into versatile heterologous protein expression platforms. Earlier works showed that Ogataea thermomethanolica TBRC 656 (OT), a thermotolerant methylotrophic yeast, can efficiently produce several i...
CRISPR-Cas9-Mediated NPC1 Gene Deletion Enhances HEK 293 T Cell Adhesion by Regulating E-Cadherin [0.03%]
基于CRISPR-Cas9的NPC1基因敲除通过调节E-钙黏蛋白增强HEK 293 T细胞粘附作用的研究
Zisen Jia,Minlin Yang,Yanchun Zhao et al.
Zisen Jia et al.
NPC1 gene encodes a transmembrane glycoprotein on the late endosome/lysosomal membrane. Its mutation leads to a rare and aggravated autosomal recessive neurovisceral condition, termed Niemann-Pick disease type C1 (NPC1), which is characteri...