Regulatory Mechanisms, Protein Expression and Biological Activity of Photolyase Gene from Spodoptera littoralis Granulovirus Genome [0.03%]
Spodoptera littoralis颗粒体病毒基因组中光复活酶基因的调控机制、蛋白表达及其生物活性研究
Wael Elmenofy,Lamiaa El-Gaied,Reda Salem et al.
Wael Elmenofy et al.
One of the most important factor that affects the efficient using of baculoviruses as a biopesticide is their sensitivity to UV irradiation. In this study, a photolyase gene (phr) of 1.4 kbp DNA fragment was cloned and characterized from Sp...
CRISPR and Antisense RNA Technology: Exploiting Nature's Tool to Restrain Virulence in Tenacious Pathogens [0.03%]
CRISPR与反义RNA技术：利用自然工具抑制顽固病原菌的致病性
Priyanka Ashwath,Disha Somanath,Akhila Dharnappa Sannejal
Priyanka Ashwath
Pathogenic bacteria constitute a significant threat to mankind and at the same time represent a huge reservoir of abeyant therapeutics to prevent and treat various diseases. The concept of virulence determinants has been a compelling tool i...
Laser Microdissection of Woody and Suberized Plant Tissues for RNA-Seq Analysis [0.03%]
用于RNA测序分析的木质和栓质植物组织的激光显微切割技术
Rita Costa Pires,Ana Ferro,Tiago Capote et al.
Rita Costa Pires et al.
An accurate profile of gene expression at a cellular level can contribute to a better understanding of biological processes and complexities involved in regulatory mechanism of woody plants. Laser microdissection is one technique that allow...
Expression and Purification of Porcine Rotavirus Structural Proteins in Silkworm Larvae as a Vaccine Candidate [0.03%]
家蚕表达纯化猪轮状病毒结构蛋白作为疫苗候选者
Tatsuya Kato,Tatsuki Kakuta,Ami Yonezuka et al.
Tatsuya Kato et al.
In this study, silkworm larvae were used for expression of porcine rotavirus A (KS14 strain) inner capsid protein, VP6, and outer capsid protein, VP7. Initially, VP6 was fused with Strep-tag II and FLAG-tag (T-VP6), and T-VP6 was fused furt...
DNA Barcoding of Prunus Species Collection Conserved in the National Gene Bank of Egypt [0.03%]
保存在中国埃及国家基因库的樱桃李属种质资源的DNA条形码研究
Hossam A Sayed,Shimaa Mostafa,Ibrahim M Haggag et al.
Hossam A Sayed et al.
Two intergenic spacers cpDNA barcoding regions were used to assess the genetic diversity and phylogenetic structure of a collection of 25 Prunus accessions. The trnH-psbA and trnL-trnF intergenic spacers were able to distinguish and identif...
CircSHOC2 Knockdown Alleviates High Glucose-Induced Vascular Endothelial Cell Pyroptosis via Targeting miR-145/FOXO1 Axis In Vitro Condition [0.03%]
circshoc2通过靶向miR-145/FOXO1轴缓解高糖诱导的血管内皮细胞焦亡的体外研究
Xuying Meng,Zhenjin Li,Yang Li
Xuying Meng
Emerging evidence indicates that pyroptosis participates in the pathogenic process of vascular endothelial cells in cardiovascular system complications of diabetes. The roles of circular RNAs (circRNAs) in high glucose (HG)-induced vascular...
Comparative Study of Elabela and Apelin on Apelin Receptor Activation Through β-Arrestin Recruitment [0.03%]
Elabela与Apelin激活Apelin受体介导的β- Arrestin募集的比较研究
Hong Zhang,Juan Chen,Min Shi et al.
Hong Zhang et al.
Apelin receptor (APJ) ligands elabela (ELA) and apelin have divergent distributions and function differently in vitro and in vivo. Whether differences exist in their capacity of recruitment of β-arrestins (ARRBs) to APJ remains unknown. Th...
Comparative Study
Molecular biotechnology. 2023 Mar;65(3):394-400. DOI:10.1007/s12033-022-00529-6 2023
Optimized Rhodobacter sphaeroides for the Production of Antioxidants and the Pigments with Antioxidant Activity [0.03%]
优化产抗氧化剂和具有抗氧化活性的色素的紫色非硫细菌RHODOBACTER SPHAEROIDES菌株
Subin Lee,Jaeyoung Yu,Yang-Hoon Kim et al.
Subin Lee et al.
The photosynthetic bacterium, Rhodobacter sphaeroides, is a bacterium that can grow in a variety of environments and produces substances with antioxidant effects. In this study, we investigated the culture conditions to increase the product...
Current and Prospective Applications of CRISPR-Cas12a in Pluricellular Organisms [0.03%]
CRISPR-Cas12a在 multicellular 组织细胞中的现状及展望
Shaheen Khan,Erwan Sallard
Shaheen Khan
CRISPR-Cas systems play a critical role in the prokaryotic adaptive immunity against mobile genetic elements, such as phages and foreign plasmids. In the last decade, Cas9 has been established as a powerful and versatile gene editing tool. ...
Ujjayani Saha,Rashmi Gondi,Amrita Patil et al.
Ujjayani Saha et al.
The ESKAPE (Enterococcus spp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) isolates both from the clinical settings and food products are demonstrated to gain resista...