A Simple Tube Escape Assay to Test Learning and Memory in Zebrafish with Minimized Habituation [0.03%]
一种简单的管逃生实验，用于测试斑马鱼的学习和记忆功能并最小化习惯化的影响
Dilan Wellalage Don,Tae-Yoon Kim,Bin Na Hong et al.
Dilan Wellalage Don et al.
Various methods have been used in rodents to evaluate learning and memory. Although much less frequently used, the zebrafish emerges as an alternative model organism in this context. For example, it allows assessing potential behavioral def...
Madelyn M Jackstadt,Lara Hutson,Jennifer O Liang et al.
Madelyn M Jackstadt et al.
Editorial
Zebrafish. 2024 Apr;21(2):71-72. DOI:10.1089/zeb.2024.0141 2024
Fipronil Affects Craniofacial and Heart Development in Zebrafish Embryos (Danio rerio) [0.03%]
伏虫隆影响斑马鱼胚胎颅面和心脏的发育
Kasey L Cooper,Zoe G Krut,Bennet D Franz et al.
Kasey L Cooper et al.
Fipronil is a broad-spectrum insecticide that has off-target effects in developing vertebrate embryos. In this study, we investigate treatment of zebrafish embryos with fipronil over the course of 5 days and examine the effects on body leng...
β-Estradiol Supplementation Regulates Cholesterol Synthesis Independent of Unsaturated Fat Consumption in Adult Zebrafish [0.03%]
雌二醇补充剂调节成年斑马鱼的胆固醇合成独立于不饱和脂肪摄入量
Rachel V Yinger,Colin J Evangelisto,Nicholas C Tucker et al.
Rachel V Yinger et al.
Obesity is a public health concern resulting in a variety of health complications, including heart disease and insulin resistance. Estrogens have been associated with a reduced risk of obesity, but this relationship remains incompletely und...
Growing Project BioEYES: A Reflection on 20 Years of Developing and Replicating a K-12 Science Outreach Program [0.03%]
见证成长：二十年K-12科学普及项目BioEYES的经验与反思
Jamie R Shuda,Valerie G Butler,Theresa M Nelson et al.
Jamie R Shuda et al.
Project BioEYES celebrated 20 years in K12 schools during the 2022-2023 school year. Using live zebrafish (Danio rerio) during week-long science experiments, sparks the interest of students and teachers from school districts, locally and gl...
Real-Time Observation of Germinal Vesicle Migration During Oocyte Meiotic Cell Division Using Ovarian Fluorescent Transgenic Zebrafish [0.03%]
应用卵巢荧光转基因斑马鱼实时观察卵母细胞减数分裂时生发泡的迁移过程
Eisei Tsutsumi,Toshinobu Tokumoto
Eisei Tsutsumi
The transgenic (TG) zebrafish allows researchers to bio-image specific biological phenomena in cells and tissues in vivo. We established TG lines to monitor changes in the ovaries of live fish. The original TG line with ovarian fluorescence...
An Undergraduate Course in CRISPR/Cas9-Mediated Gene Editing in Zebrafish [0.03%]
CRISPR/Cas9基因编辑斑马鱼本科课程
Renu Srivastava,Connor W Davison,Abigail G Krull et al.
Renu Srivastava et al.
We have developed a one-credit semester-long research experience for undergraduate students that involves the use of CRISPR/Cas9 to edit genes in zebrafish. The course is available to students at all stages of their undergraduate training a...
Zachary Oglesby,Alanna Nicole Rillorta,Cheydon Agos et al.
Zachary Oglesby et al.
The Ala Wai Canal is an artificial waterway in the tourist district of Waikiki in Honolulu, HI. Originally built to collect runoff from industrial, residential, and green spaces dedicated to recreation, the Ala Wai Canal has since experienc...
Laboratory Course Using Zebrafish to Uncover Changing Roles of Wnt Signaling in Early Vertebrate Development [0.03%]
利用斑马鱼实验课程揭秘Wnt信号在早期脊椎动物发育中的变化作用
Manuel Fiegl,Robin A Kimmel
Manuel Fiegl
Coordinated signaling pathway activity directs early patterning to set up the vertebrate body plan. Perturbations in the timing or location of signal molecule expression impacts embryo morphology and organ formation. In this study, we prese...
The InSciEdRS View: A User-Friendly and Accessible Microscope with Easy-to-Follow Companion Curricula [0.03%]
InSciEdUp观点：用户友好且便于获取的显微镜及其配套教学大纲
Soaleha Shams,Sidney Olson,Michael P Ekker et al.
Soaleha Shams et al.
Microscopes are essential for research and education in science. Unlike computers and online learning tools, however, microscopes are not currently a fixed element in K-12 classrooms, due to steep cost, needless complexity, and often requir...