An Improved, High-Yield Method for Isolating Nuclei from Individual Zebrafish Embryos for Single-Nucleus RNA Sequencing [0.03%]
用于斑马鱼胚胎单核RNA测序的一种改进的、高产量的分离细胞核的方法
Clifford Rostomily,Heidi Lee,Amy Tresenrider et al.
Clifford Rostomily et al.
Zebrafish is an ideal system to study the effect(s) of chemical, genetic, and environmental perturbations on development due to their high fecundity and fast growth. Recently, single-cell sequencing has emerged as a powerful tool to measure...
Renata Villar Dantas,Lis Santos Marques,Thaiza Rodrigues Freitas et al.
Renata Villar Dantas et al.
This study aimed to evaluate the viability of gametes in zebrafish (Danio rerio), at different rigor mortis stages. Viability assessments were conducted on oocytes at various developmental stages using LIVE/DEAD and the 3-(4,5-dimethylthiaz...
Small Fishes, Big Issues: Species Delimitation in Hemigrammus Marginatus, Gill, 1958 (Acestrorhamphidae: Pristellinae) from Brazilian Coastal Basins Based on Integrative Genetics [0.03%]
小鱼，大问题：基于综合遗传学对1958年Gill的巴西海岸盆地Hemigrammus Marginatus（Acestrorhamphidae: Pristellinae）物种界限的划分
Mauricio B Fernandes,Jamille De Araújo Bitencourt,André Teixeira Da Silva et al.
Mauricio B Fernandes et al.
The small characins represent a systematic puzzle in the Neotropical ichthyofauna as a result of independent miniaturization processes, adaptive convergence and lack of diagnostic characters for several genera. In order to diminish the taxo...
In Vivo Measurement of Intestinal Permeability to Macromolecules in Adult Zebrafish (Danio rerio) [0.03%]
成人斑马鱼（丹尼罗氏斑鱼）体内大分子肠道通透性的活体测量
Marcela G Meirelles,Camila I M Fénero,Bruna F Nornberg et al.
Marcela G Meirelles et al.
Intestinal permeability plays a crucial role in intestinal barrier function. Altered intestinal permeability is well documented in numerous chronic diseases and may serve as a risk factor for disease onset as well as a target for innovative...
An Image Processing Tool for Automated Quantification of Bacterial Burdens in Zebrafish Larvae [0.03%]
一种用于自动化定量斑马鱼幼虫细菌负荷的图像处理工具
Naoya Yamaguchi,Hideo Otsuna,Michal Eisenberg-Bord et al.
Naoya Yamaguchi et al.
Zebrafish larvae are used to model the pathogenesis of multiple bacteria. This transparent model offers the unique advantage of allowing quantification of fluorescent bacterial burdens (fluorescent pixel counts [FPC]) in vivo by facile micr...
An Automated Low-Cost Solution for Creating a Multiple-Step Thermal Gradient to Record Daily Fish Thermoregulatory Behavior [0.03%]
一种自动化的低成本解决方案，用于创建多步热梯度以记录鱼类每日的温度调节行为
Francesca Conti,Gonzalo de Alba,Jose F López-Olmeda et al.
Francesca Conti et al.
Photoperiod and temperature are two of the most powerful environmental cues that entrain circadian clocks. Being ectothermic, fish must keep their body temperature within a physiological range to optimize biological processes mainly applyin...
Candidate Gene Expression in Adult Zebrafish Models of Type 2 Diabetes Mellitus [0.03%]
2型糖尿病成人斑马鱼模型中的候选基因表达分析
Mlondi Shezi,Celia Snyman,Carola Ulrike Niesler
Mlondi Shezi
Animal models are an important tool for studying noncommunicable diseases (NCDs) as they provide a unique opportunity to investigate real-time changes that occur in the onset of, and during, the diseased state. This is of particular importa...
Zebrafish as a Model Organism for Congenital Hydrocephalus: Characteristics and Insights [0.03%]
斑马鱼在先天性脑积水研究中的模式生物特性及启示
Kaiyue Wang,Zhi Tang,Yijian Yang et al.
Kaiyue Wang et al.
Hydrocephalus is a cerebrospinal fluid-related disease that usually manifests as abnormal dilation of the ventricles, with a triad of clinical findings including walking difficulty, reduced attention span, and urinary frequency or incontine...
Advancing Zebrafish Husbandry: Takeaways From the 2024 Husbandry Workshop and Husbandry Summit [0.03%]
斑马鱼饲养技术的提升：2024年饲养工作坊与饲养峰会精华总结
Michelle Altemara,Bobbi Baur,Zoltan M Varga
Michelle Altemara
The 2024 Zebrafish Husbandry Workshop and Summit held during the World Aquaculture Society Meeting in San Antonio focused on key areas for improving zebrafish husbandry research. Discussions highlighted the need for comprehensive literature...