Kai Hao,Yunzheng Liu,Mykel Barrett et al.
Kai Hao et al.
Intensity and duration of biological signals encode a few pathways to direct diverse cellular behaviors, yet quantifying these features in single cells remains difficult. To address this challenge, we developed INSCRIBE, which uses a CRISPR...
Structures of ALG3/9/12 reveal the assembly logic of the N-glycan oligomannose core [0.03%]
ALG3/9/12的结构揭示N-糖链寡甘露糖核心的组装逻辑
J Andrew N Alexander,Shu-Yu Chen,Somnath Mukherjee et al.
J Andrew N Alexander et al.
Asparagine-linked glycans are essential for the maturation and function of most eukaryotic secretory proteins. The biosynthesis and transfer of dolichylpyrophosphate-anchored GlcNAc2Man9Glc3 glycan is a highly conserved process occurring in...
Hetero-oligomerization drives structural plasticity of eukaryotic peroxiredoxins [0.03%]
异寡聚作用驱动真核过氧化物还原酶的结构可塑性
Jannik Zimmermann,Lukas Lang,Julia Malo Pueyo et al.
Jannik Zimmermann et al.
Peroxiredoxins are thiol peroxidases, which detoxify peroxides, relay redox signals and act as chaperones. In eukaryotes, multiple peroxiredoxin-1 (Prx1)/AhpC-type isoforms frequently co-exist in the same subcellular compartment, yet have b...
Hortense Mazon,Benjamin Selles,Sophie Rahuel-Clermont
Hortense Mazon
Junfeng Ma,Chunyan Hou
Junfeng Ma
Mast cell extracellular granules are bioactive condensates assembled by heparin and polyamine [0.03%]
肥大细胞的胞外小体由肝素和多胺组装而成的生物活性凝聚体
Yiwei Xiong,Dylan T Tomares,Jianjian Guo et al.
Yiwei Xiong et al.
Biomolecular condensates are membraneless bodies that organize biochemical reactions typically within cells. However, the roles of condensates in extracellular space-where conditions differ substantially from intracellular space-remain poor...
Defining and refining the cysteine redoxome through sulfur chemical biology [0.03%]
硫化学生物学定义和精炼半胱氨酸氧化还原组学
Kate S Carroll,Jing Yang
Kate S Carroll
Cysteine is one of the rarest amino acids yet exerts a profound influence on biology through the exceptional chemistry of its thiol group. Tunable acidity, high nucleophilicity and access to multiple oxidation states position cysteine as bo...
Grip it and rip it [0.03%]
抓住它，然后快速行动
Thomas A Laskarzewski,Thomas J Maresca
Thomas A Laskarzewski
Designing chemigenetic DNA nanotrap for norepinephrine dynamic imaging in organelles [0.03%]
设计用于细胞器中多巴胺动态成像的化学遗传DNA纳米陷阱
Yilin Chen,Zhichao Liu,Yating Wang et al.
Yilin Chen et al.
The lack of organelle-targeted neurotransmitter probes limits understanding of their intracellular roles. Here we created an organelle-targeted neurotransmitter nanoprobe using specific molecule-trapped DNA nanostructures for multiple recog...