Structure, sequon recognition and mechanism of tryptophan C-mannosyltransferase [0.03%]
色氨酸C-甘露糖基转移酶的结构、序列识别及机制
Joël S Bloch,Alan John,Runyu Mao et al.
Joël S Bloch et al.
C-linked glycosylation is essential for the trafficking, folding and function of secretory and transmembrane proteins involved in cellular communication processes. The tryptophan C-mannosyltransferase (CMT) enzymes that install the modifica...
Structural basis for heparan sulfate co-polymerase action by the EXT1-2 complex [0.03%]
EXT1-2复合体通过肝素硫酸共聚合酶作用的结构基础
Hua Li,Digantkumar Chapla,Robert A Amos et al.
Hua Li et al.
Heparan sulfate (HS) proteoglycans are extended (-GlcAβ1,4GlcNAcα1,4-)n co-polymers containing decorations of sulfation and epimerization that are linked to cell surface and extracellular matrix proteins. In mammals, HS repeat units are e...
Co-evolution-based prediction of metal-binding sites in proteomes by machine learning [0.03%]
基于共进化和机器学习的蛋白质组金属结合位点预测
Yao Cheng,Haobo Wang,Hua Xu et al.
Yao Cheng et al.
Metal ions have various important biological roles in proteins, including structural maintenance, molecular recognition and catalysis. Previous methods of predicting metal-binding sites in proteomes were based on either sequence or structur...
Charlene Bashore,Sumit Prakash,Matthew C Johnson et al.
Charlene Bashore et al.
Engineered destruction of target proteins by recruitment to the cell's degradation machinery has emerged as a promising strategy in drug discovery. The majority of molecules that facilitate targeted degradation do so via a select number of ...
CIDE-stepping E3s [0.03%]
CIDE步进E3蛋白泛素连接酶家族
Gary Tin,Georg E Winter
Gary Tin
A change of phase [0.03%]
相变
Anita Silver,Lingchong You
Anita Silver
Wentao Kong,Yuanchao Qian,Philip S Stewart et al.
Wentao Kong et al.
Synthetic biology has shown remarkable potential to program living microorganisms for applications. However, a notable discrepancy exists between the current engineering practice-which focuses predominantly on planktonic cells-and the ubiqu...
Jonathan M Ellis,Andrew R Buller
Jonathan M Ellis
Designer installation of a substrate recruitment domain to tailor enzyme specificity [0.03%]
通过设计安装底物招募域来定制酶的特异性
Rodney Park,Chayanid Ongpipattanakul,Satish K Nair et al.
Rodney Park et al.
Promiscuous enzymes that modify peptides and proteins are powerful tools for labeling biomolecules; however, directing these modifications to desired substrates can be challenging. Here, we use computational interface design to install a su...
An E. coli display method for characterization of peptide-sensor kinase interactions [0.03%]
一种用于表征肽-传感器激酶相互作用的E.coli 显示方法
Kathryn R Brink,Maxwell G Hunt,Andrew M Mu et al.
Kathryn R Brink et al.
Bacteria use two-component system (TCS) signaling pathways to sense and respond to peptides involved in host defense, quorum sensing and inter-bacterial warfare. However, little is known about the broad peptide-sensing capabilities of TCSs....