Unconventional monooxygenation by the O2-dependent tRNA wobble uridine hydroxylase TrhO [0.03%]
由分子氧依赖的tRNA颠换尿苷羟基酶TrhO催化的一种非传统单加氧反应
Kiroo Shin,Da Bean Han,Hyun Woo Kim et al.
Kiroo Shin et al.
Modifications at the wobble position of transfer RNA (tRNA) are critical for accurate codon recognition and efficient translation. 5-Hydroxyuridine serves as a key intermediate for more complex wobble uridine derivatives commonly found in b...
Ideal efficacy photoswitching for chromocontrol of TRPC4/5 channel functions in live tissues [0.03%]
用于活体组织中TRPC4/5通道功能的理想功效光电转换法
Markus Müller,Konstantin Niemeyer,Navin K Ojha et al.
Markus Müller et al.
Precisely probing the endogenous roles of target proteins is crucial for biological research. Photochemical tools can be photoactuated with high spatiotemporal resolution but often they are unreliable in vivo because spatiotemporal variatio...
Jana Volarić,Wiktor Szymanski
Jana Volarić
Autopalmitoylation of IDH1-R132H regulates its neomorphic activity in cancer cells [0.03%]
IDH1-R132H 自棕榈酰化调节其在癌细胞中的新表型活性
Lu Hu,Jinyu Lin,Liping Sun et al.
Lu Hu et al.
Gain-of-function mutations of isocitrate dehydrogenase 1 (IDH1) lead to oncometabolite (R)-2-hydroxyglutarate production, contributing to the tumorigenesis of multiple human cancers. While fatty acid biosynthesis is critical for IDH1-mutant...
GCN5-ERK lactylation-phosphorylation loop amplifies lactate-driven cancer progression [0.03%]
GCN5-ERK乳酸化-磷酸化循环放大了乳酸驱动的癌症进展
Bingsong Huang,Mingpeng Jin,Gaofeng Cui et al.
Bingsong Huang et al.
The Warburg effect leads to increased lactate production and promotes cancer progression but the underlying mechanisms remain unclear. Here, we found that lactate activates the MAPK pathway through ERK lactylation, which promotes cancer pro...
Catherine A Musselman,Tatiana G Kutateladze
Catherine A Musselman
LipoID profiles lipid droplet interactions and identifies interorganelle regulators [0.03%]
LipoID鉴定脂滴相互作用及跨细胞器调节因子
Hengke Guo,Wang Wan,Yanan Huang et al.
Hengke Guo et al.
Lipid droplets (LDs) dynamically interact with other organelles, such as mitochondria, in surveillance of cellular metabolic homeostasis. The transient nature of LDs, however, poses technical challenges to snapshot molecular information und...
Unique gluing effect of ASXL1 K351 monoubiquitination stimulates the PR-DUB activity [0.03%]
ASXL1 K351 单泛素化独特的胶水作用促进PR-DUB活性
Tianyi Zhang,Jiqing Zheng,Zebing Tong et al.
Tianyi Zhang et al.
Protein ubiquitination critically regulates biological processes through both proteolytic and nonproteolytic mechanisms. While classically known for protein degradation, ubiquitination also modulates enzymatic activity. However, current mec...
Yuefeng Ma,Leshan Yang,Yantong Chen et al.
Yuefeng Ma et al.
Engineering synthetic intrinsically disordered proteins (synIDPs) enables regulation of biomolecular condensation and protein solubility. However, limited understanding of how sequence-dependent interaction cooperativity relates to the fitn...