Development of a highly efficient gene targeting system induced by transient repression of YKU80 expression in Candida glabrata [0.03%]
通过瞬时抑制YKU80表达高效诱导基因定位技术在光滑念珠菌中的发展研究论文
Keigo Ueno,Jun Uno,Hironobu Nakayama et al.
Keigo Ueno et al.
In the pathogenic yeast Candida glabrata, gene targeting to generate knockouts and "knockins" is a potentially powerful method for the analysis of gene function. Its importance increased after the C. glabrata genome sequence project, but pr...
Genomic and population analyses of the mating type loci in Coccidioides species reveal evidence for sexual reproduction and gene acquisition [0.03%]
火谷属菌种的交配型位点的基因组和群体分析揭示了性繁殖和基因获取的证据
M Alejandra Mandel,Bridget M Barker,Scott Kroken et al.
M Alejandra Mandel et al.
Coccidioides species, the fungi responsible for the valley fever disease, are known to reproduce asexually through the production of arthroconidia that are the infectious propagules. The possible role of sexual reproduction in the survival ...
Protein trafficking to the apicoplast: deciphering the apicomplexan solution to secondary endosymbiosis [0.03%]
顶质体蛋白质输送机制及阿米巴虫门解决次级内共生的方案
Marilyn Parsons,Anuradha Karnataki,Jean E Feagin et al.
Marilyn Parsons et al.
Characterization of three classes of membrane proteins involved in fungal azole resistance by functional hyperexpression in Saccharomyces cerevisiae [0.03%]
通过在Saccharomyces cerevisiae中功能高表达表征三类与真菌唑类抗性相关的膜蛋白
Erwin Lamping,Brian C Monk,Kyoko Niimi et al.
Erwin Lamping et al.
The study of eukaryotic membrane proteins has been hampered by a paucity of systems that achieve consistent high-level functional protein expression. We report the use of a modified membrane protein hyperexpression system to characterize th...
Use of recombinant Entamoeba histolytica cysteine proteinase 1 to identify a potent inhibitor of amebic invasion in a human colonic model [0.03%]
用人结肠模型筛选溶组织内阿米巴囊泡蛋酶-1重组抗侵袭剂的研究
Samuel G Meléndez-López,Scott Herdman,Ken Hirata et al.
Samuel G Meléndez-López et al.
Cysteine proteinases are key virulence factors of the protozoan parasite Entamoeba histolytica. We have shown that cysteine proteinases play a central role in tissue invasion and disruption of host defenses by digesting components of the ex...
Split-ubiquitin two-hybrid assay to analyze protein-protein interactions at the endosome: application to Saccharomyces cerevisiae Bro1 interacting with ESCRT complexes, the Doa4 ubiquitin hydrolase, and the Rsp5 ubiquitin ligase [0.03%]
用于分析囊泡末次体蛋白质间相互作用的裂解泛素双杂交测定法及其在模式生物假丝酵母Bro1与ESCRT复合物、Doa4去泛素化酶及Rsp5泛素连接酶相互作用中的应用
Elina Nikko,Bruno André
Elina Nikko
Targeting of membrane proteins into the lysosomal/vacuolar lumen for degradation requires their prior sorting into multivesicular bodies (MVB). The MVB sorting pathway depends on ESCRT-0, -I, -II, and -III protein complexes functioning on t...
Localization of RHO-4 indicates differential regulation of conidial versus vegetative septation in the filamentous fungus Neurospora crassa [0.03%]
RHO-4的定位表明了粗糙脉胞菌中分生孢子形成与营养生长的隔膜化过程具有不同的调节机制
Carolyn G Rasmussen,N Louise Glass
Carolyn G Rasmussen
Rho-4 mutants of the filamentous fungus Neurospora crassa lack septa and asexual spores (conidia) and grow slowly. In this report, localization of green fluorescent protein-tagged RHO-4 is used to elucidate the differences in factors contro...
Host factors that control long terminal repeat retrotransposons in Saccharomyces cerevisiae: implications for regulation of mammalian retroviruses [0.03%]
控制酿酒酵母长末端重复序列转座子的宿主因素及其对哺乳动物反转录病毒调节的启示
Patrick H Maxwell,M Joan Curcio
Patrick H Maxwell
Two novel, putatively cell wall-associated and glycosylphosphatidylinositol-anchored alpha-glucanotransferase enzymes of Aspergillus niger [0.03%]
两项新的假定的与细胞壁相连和糖基磷脂酰肌醇锚定的α-葡萄聚糖转移酶类酶的曲霉菌物种新型蛋白质编码基因AN15803和AN4629的研究报告
R M van der Kaaij,X-L Yuan,A Franken et al.
R M van der Kaaij et al.
In the genome sequence of Aspergillus niger CBS 513.88, three genes were identified with high similarity to fungal alpha-amylases. The protein sequences derived from these genes were different in two ways from all described fungal alpha-amy...
Development and application of a green fluorescent protein sentinel system for identification of RNA interference in Blastomyces dermatitidis illuminates the role of septin in morphogenesis and sporulation [0.03%]
白色念珠菌RNA干扰技术的建立及其在Septin基因功能分析中的应用
T Krajaejun,G M Gauthier,C A Rappleye et al.
T Krajaejun et al.
A high-throughput strategy for testing gene function would accelerate progress in our understanding of disease pathogenesis for the dimorphic fungus Blastomyces dermatitidis, whose genome is being completed. We developed a green fluorescent...