Improved accumulation of betulin and betulinic acid in cell suspension culture of Betula pendula roth by abiotic and biotic elicitors [0.03%]
白桦素和桦木酸生物及非生物诱导剂的累积改善
Razieh Jafari Hajati,Vahide Payamnoor,Najmeh Ahmadian Chashmi et al.
Razieh Jafari Hajati et al.
Betulin (B) and betulinic acid (BA) are two triterpenes with diverse pharmacological and physiological actions. Elicitation of Betula pendula Roth cell cultures by elicitors is an excellent strategy to increase B and BA levels. Six abiotic ...
Candida rugosa lipase encapsulated with magnetic sporopollenin: design and enantioselective hydrolysis of racemic arylpropanoic acid esters [0.03%]
磁性花粉壁质封装的星形皮炎毛癣菌脂肪酶的设计及芳基丙酸酯消旋体的拆分水解反应
Elif Ozyilmaz,Kubra Etci,Mehmet Sezgin
Elif Ozyilmaz
The aim of this study was to prepare the encapsulation of Candida rugosa lipase (CRL) with magnetic sporopollenin. The sporopollenin was covalent immobilized onto magnetic nanoparticles (Fe3O4), grafted amino (APTES), or epoxy groups (EPPTM...
Expression, purification, and characterization of N-terminal His-tagged proteins with mutations in zinc finger 3 of zinc finger protein ZNF191(243-368) [0.03%]
锌指蛋白ZNF191（243-368）中第3个C₂H₂型锌指突变体N端His标签融合蛋白质的表达、纯化与鉴定
Dongxin Zhao,Zhongxian Huang,Jie Liu et al.
Dongxin Zhao et al.
Zinc finger protein ZNF191(243-368), the zinc finger region of ZNF191, is potentially associated with cell proliferation in hepatocellular carninoma. A His-tag expression system was used to express and purify proteins with mutations in the ...
A novel amperometric bienzymatic biosensor based on alcohol oxidase coupled PVC reaction cell and nanomaterials modified working electrode for rapid quantification of alcohol [0.03%]
一种基于醇氧化酶的新型安培双酶生物传感器定量测定酒精含量
Vinita Hooda,Vikas Kumar,Anjum Gahlaut et al.
Vinita Hooda et al.
A new amperometric sensor has been fabricated for sensitive and rapid quantification of ethanol. The biosensor assembly was prepared by covalently immobilizing alcohol oxidase (AOX) from Pichia pastoris onto chemically modified surface of p...
Immobilization and characterization of lipase from an indigenous Bacillus aryabhattai SE3-PB isolated from lipid-rich wastewater [0.03%]
从富含脂质的废水中分离土著Bacillus aryabhattai SE3-PB的脂肪酶的固定化及特征分析
Adegoke Isiaka Adetunji,Ademola Olufolahan Olaniran
Adegoke Isiaka Adetunji
Extracellular lipase from an indigenous Bacillus aryabhattai SE3-PB was immobilized in alginate beads by entrapment method. After optimization of immobilization conditions, maximum immobilization efficiencies of 77% ± 1.53% and 75.99% ± 3...
Production of γ-aminobutyric acid in Escherichia coli by engineering MSG pathway [0.03%]
通过工程化MSG途径在大肠杆菌中生产γ-氨基丁酸
Ping Yu,Kaifei Chen,Xingxing Huang et al.
Ping Yu et al.
The compound γ-aminobutyric acid (GABA) has many important physiological functions. The effect of glutamate decarboxylases and the glutamate/GABA antiporter on GABA production was investigated in Escherichia coli. Three genes, gadA, gadB, ...
Prospecting of soybean hulls as an inducer carbon source for the cellulase production [0.03%]
筛选大豆皮作为纤维素酶发酵的诱导碳源
Thayná G Fernandes,Jorge A López,Luana A Silva et al.
Thayná G Fernandes et al.
Cellulases constitute an enzymatic complex involved in the cellulose hydrolysis β-1, 4-glycosidic linkages to release of glucose. Therefore, its application to degrade agro-industrial residues becomes relevant, since glucose is a product o...
A combined treatment using ethylmethane sulfonate and ultraviolet light to compare amylase production by three Bacillus sp. isolates [0.03%]
乙基甲烷磺酸联合紫外线处理三种芽孢杆菌分离株淀粉酶产生的比较分析
Betul Zehra Karakus,İlknur Korkmaz,Kubra Demirci et al.
Betul Zehra Karakus et al.
In this study, three Bacillus sp.-producing amylase enzymes were isolated from soil samples and identified using 16S rDNA sequence analysis. Amylase production and total protein productions were spectrophotometrically measured. The followin...
An effective, simple and low-cost pretreatment for culture clarification in tetanus toxoid production [0.03%]
破伤风毒素生产用有效的简易低成本培养液澄清预处理方法
Lucía Avila,Osvaldo Cascone,Mirtha Biscoglio et al.
Lucía Avila et al.
Chemically inactivated tetanus toxin (tetanus toxoid, TT), purified from cultures of a virulent Clostridium tetani strain, is the active pharmaceutical ingredient of anti-tetanus vaccines. Culture clarification for TT production and is usua...
Cansu Sunguroğlu,Dilber Ece Sezgin,Pınar Aytar Çelik et al.
Cansu Sunguroğlu et al.
Hyaluronic acid (HA) is a natural biopolymer and has long been attracting the attention of biotechnology industry due to its various biological functions. HA production with natural producer Streptococcus equi subsp. zooepidemicus has not b...