Optimization of culture conditions and bench-scale production of anticancer enzyme L-asparaginase by submerged fermentation from Aspergillus terreus CCT 7693 [0.03%]
基于产黄曲霉液体发酵法生产抗癌酶L-天冬酰胺酶的工艺优化及中试研究
T A Costa-Silva,D I Camacho-Córdova,G S Agamez-Montalvo et al.
T A Costa-Silva et al.
L-Asparaginase amidohydrolase (EC 3.5.1.1) has received significant attention owing to its clinical use in acute lymphoblastic leukemia treatment and non-clinical applications in the food industry to reduce acrylamide (toxic compound) forma...
A comparative study of the bispecific monoclonal antibody, blinatumomab expression in CHO cells and E. coli [0.03%]
毕诺莫单抗在CHO细胞和大肠杆菌中表达的比较研究
Fatemeh Naddafi,Farshad H Shirazi,Yeganeh Talebkhan et al.
Fatemeh Naddafi et al.
The "bispecifics" market improved over the past decade due to the development of many technological platforms including bispecific T cell engagers (BiTEs). The approval of blinatumomab, the most advanced bispecific T-cell engager (BiTE) in ...
Stability and structure of Penicillium chrysogenum lipase in the presence of organic solvents [0.03%]
有机溶剂存在下青霉素G产生菌脂肪酶的稳定性和结构研究
Ayesha Sadaf,Jasneet Grewal,Isha Jain et al.
Ayesha Sadaf et al.
The present work describes the enzymatic properties of Penicillium chrysogenum lipase and its behavior in the presence of organic solvents. The temperature and pH optima of the purified lipase was found to be 55 °C and pH 8.0 respectively....
Preparative enrichment of human tissue cells capable to change a site of growth in vitro or in vivo - Recent developments [0.03%]
用于体外或体内生长部位改变的人类组织细胞的制备富集法-最近的发展
Johann Bauer,Hari H P Cohly,Jayashree Sahana et al.
Johann Bauer et al.
Human cells are heterogeneous in regard to their biochemical features and functions. Detailed knowledge about each single cell type is important to understand the whole organism. In order to get a deeper insight in the concert of life, it h...
Production and characterization of a conserved M2e peptide-based specific IgY antibody: evaluation of the diagnostic potential via conjugation with latex nanoparticles [0.03%]
基于M2e保守肽诱导的IgY抗体的制备、鉴定及偶联latex胶体金的诊断价值评估研究
Yasemin Budama-Kilinc,Rabia Cakir-Koc,Burak Ozdemir et al.
Yasemin Budama-Kilinc et al.
Antibodies play an important role in combating and controlling viral diseases such as influenza. Immunoglobulin Y (IgY) antibodies have several advantages such as a less invasive manufacturing process, ease of isolation, higher affinity com...
Stability of immobilized laccase on Luffa Cylindrica fibers and assessment of synthetic hormone degradation [0.03%]
黄瓜纤维固定化漆酶的稳定性及其对人工合成激素降解效果的评估
Monike Fabiane Alves Ribeiro Lacerda,Flávio Marques Lopes,Adilson Sartoratto et al.
Monike Fabiane Alves Ribeiro Lacerda et al.
In this work were studied the pH, thermal, and storage stability of free and immobilized laccases. Enzymes were produced by Pleurotus ostreatus on potato dextrose (PD) broth and potato dextrose modified (PDM) broth, and immobilized using Lu...
Solid-liquid extraction of daidzein and genistein from soybean: Kinetic modeling of influential factors [0.03%]
大豆中大豆苷和染料木素的固液萃取：影响因素的动力学模型研究
Guanjun Nan,Yuqiong Gao,Liying Guo et al.
Guanjun Nan et al.
The extraction of daidzein and genistein from soybean has been studied and the kinetic modeling was established using four modeling equations. The goodness of fit was evaluated by statistical errors including the standard error of means (SE...
Bioproduction of low-pigment xanthan gum by a cell-wall deficient mutant of Xanthomonas campestris [0.03%]
黄单胞菌外壳缺陷型突变体低色素 production 研究
Yongmei Liu,Zhonghua Wang,Mingyuan Liu et al.
Yongmei Liu et al.
This work aims to enhance the bioproduction of xanthan gum by screening a hyper-yield producer from the wild-type Xanthomonas campestris during a long-term continuous subculture. We reported a cell-wall deficient mutant, which performed a s...
In vitro investigation of hypoglycemic and oxidative stress properties of fava bean (Vicia faba L.) seed extract in Saccharomyces cerevisiae 2376 [0.03%]
辣利马豆(Vicia faba L.)种子提取物体外降糖抗氧化活性的研究
Dhiraj Kumar Choudhary,Abha Mishra
Dhiraj Kumar Choudhary
Imbalance of free radicals over antioxidants in human body may result in oxidative damage to biomolecules (lipids, proteins, DNA) that causes severe chronic diseases. The aim of this proposed research was to examine the hypoglycemic and oxi...
Solid state fermentation of Bacillus gottheilii M2S2 in laboratory-scale packed bed reactor for tannase production [0.03%]
固定床反应器中以固态发酵的方式培养芽孢杆菌生产单宁酶
Subbalaxmi Selvaraj,Ramachandra Murty Vytla
Subbalaxmi Selvaraj
Production of tannase was performed in packed bed reactor filled with an inert support polyurethane foam (PUF) using Bacillus gottheilii M2S2. The influence of process parameters such as fermentation time (24-72 h), tannic acid concentratio...