A fast and simple approach to optimize the unit operation high pressure homogenization - a case study for a soluble therapeutic protein in E. coli [0.03%]
一个优化高压均质器设计以提高可溶性治疗蛋白产量的快速而简便的方法——案例研究：大肠杆菌中表达的融合伴侣帮助下的人白细胞介素10蛋白质
Alexander Pekarsky,Oliver Spadiut,Vignesh Rajamanickam et al.
Alexander Pekarsky et al.
Escherichia coli is one of the most commonly used host organisms for the production of recombinant biopharmaceuticals. E. coli is usually characterized by fast growth on cheap media and high productivity, but one drawback is its intracellul...
Isolation, screening, and optimization of bacterial strains for novel transglutaminase production [0.03%]
新型转谷氨酰胺酶产生菌的筛选、鉴定及产酶条件优化
Karuna L Sorde,Laxmi Ananthanarayan
Karuna L Sorde
Transglutaminases are a class of transferases known to form isopeptide bond between glutamine and lysine residues in a protein molecule. Increasing demand for transglutaminase in food and other industries and its low productivity have compe...
Extracellular biosynthesis, characterization, optimization of silver nanoparticles (AgNPs) using Bacillus mojavensis BTCB15 and its antimicrobial activity against multidrug resistant pathogens [0.03%]
芽孢杆菌BTCB15合成的银纳米粒子抗菌性能研究及其抗多重耐药菌的效果
Mehwish Iqtedar,Mehral Aslam,Muhammad Akhyar et al.
Mehwish Iqtedar et al.
Biosynthesis of metal nanoparticles is an area of interest among researchers because of its eco-friendly approach. Current study focuses at biosynthesis of silver nanoparticles (AgNPs) and optimization of physico-chemical conditions to obta...
Enhanced amylase production by a Bacillus subtilis strain under blue light-emitting diodes [0.03%]
在蓝色发光二极管照射下枯草芽孢杆菌产生淀粉酶能力的增强
Punniyakotti Elumalai,Jeong-Muk Lim,Yool-Jin Park et al.
Punniyakotti Elumalai et al.
A chemotrophic, aerobic bacterial strain, Bacillus subtilis B2, was used to produce amylase by submerged fermentation under different light sources. SDS-PAGE indicated that the 55 kDa enzyme belonged to the α-amylase group. B2 was incubate...
Xuguo Duan,Zhenyan Shen,Xinyi Zhang et al.
Xuguo Duan et al.
In this study, the effects of carbon source, nitrogen source, and metal ions on cell growth and Bacillus aryabhattai β-amylase production in recombinant Brevibacillus choshinensis were investigated. The optimal medium for β-amylase produc...
Integration of size-exclusion chromatography and ultracentrifugation for purification of recombinant hepatitis B surface antigen: An alternative method for immunoaffinity chromatography [0.03%]
一种替代免疫亲和色谱法纯化重组乙肝表面抗原的方法：排阻色谱与超速离心技术联用
Mahshid Yousefipour,Maryam Khatami,Amin Javidanbardan et al.
Mahshid Yousefipour et al.
In purification process of recombinant hepatitis B surface antigen (rHBsAg), immunoaffinity chromatography (IAF) is one of the most important and effective steps in rHBsAg purification. However, the buffer composition and the interaction of...
Optimization of ultrasound-assisted water extraction of flavonoids from Psidium guajava leaves by response surface analysis [0.03%]
响应面分析超声辅助提取巴西番木瓜叶黄酮类化合物的最佳工艺优化研究
Jiaokun Li,Chenchen Wu,Fang Li et al.
Jiaokun Li et al.
Psidium guajava leaves are rich in health-promoting flavonoids compounds. For better utilization of the resource, the ultrasound-assisted aqueous extraction was investigated using Box-Behnken design under response surface methodology. A hig...
Flavonoid content and antioxidant activity of Artemisia vulgaris L. "hairy" roots [0.03%]
Artemisia vulgaris L. 毛状根中的黄酮含量及其抗氧化活性
Nadiia Matvieieva,Kateryna Drobot,Volodymyr Duplij et al.
Nadiia Matvieieva et al.
We investigated the effect of Agrobacterium rhizogenes-mediated transformation on antioxidant activity of Artemisia vulgaris "hairy" roots. It appeared that transformation may increase flavonoid content as well as DPPH-scavenging activity a...
An amylopullulanase (ApuNP1) from Geobacillus thermoleovorans NP1: biochemical characterization and its potential industrial applications [0.03%]
来源于解糖热丝链菌NP1的淀粉糊精酶（ApuNP1）：生化特性及其潜在工业应用
Nihan Arabacı,Burhan Arıkan
Nihan Arabacı
An amylopullulanase was produced by Geobacillus thermoleovorans NP1. The optimum enzyme production occurred at 45°C and pH 7.0 (12 hr). NP1 amylopullulanase (ApuNP1) exhibited the maximal activity at 50°C and pH 6.0 and was stable between...
Optimization of the 503 antigen induction strategy of Leishmania infantum chagasi expressed in Escherichia coli M15 [0.03%]
利什曼原虫503抗原在大肠杆菌M15菌株中表达的优化诱导条件研究
Luan Tales Costa de Paiva Vasconcelos,Marcos Antônio Oliveira Filho,Vitor Troccoli Ribeiro et al.
Luan Tales Costa de Paiva Vasconcelos et al.
Leishmaniosis is a complex of diseases that can be fatal, if not given proper attention. Despite its relevance in the public health system, there is no vaccine capable of preventing the disease in humans so far and its treatment is expensiv...