Purification, biochemical characterization, and industrial potential of a thermostable lipase from Aeribacillus pallidus IO3 isolated from a geothermal source [0.03%]
一株嗜热菌Aeribacillus pallidus IO3的耐热脂肪酶的纯化、生化性质及应用潜力研究
Ikra Ozkan,Ahmet Adiguzel
Ikra Ozkan
Lipases obtained from thermophilic microorganisms are attracting great interest in the industrial field due to their stability under high-temperature conditions and their wide range of industrial applications. In this study, thermophilic ba...
Optimizing the proteinase K digest improves yield and integrity of DNA extracted from formalin-fixed, paraffin-embedded tissue by an automated robot [0.03%]
优化蛋白酶K消化可提高自动机器人从甲醛固定石蜡包埋组织中提取的DNA的产量和完整性
Camille Bellora,William Mathieson
Camille Bellora
ABSTRACTAutomation is a requisite for laboratories performing large numbers of DNA extractions, but it can compromise DNA yield and/or integrity compared to manual extractions. We addressed this problem by optimizing the manual Proteinase K...
Characterization and in vitro biological activities of exopolysaccharides from sugarcane waste-derived Weissella paramesenteroides [0.03%]
蔗糖渣衍生威氏菌外多糖的性质及其体外生物活性
Poorvaja Subramanian,Preethi Kathirvel
Poorvaja Subramanian
Exopolysaccharides (EPS) are microbial biopolymers composed primarily of complex carbohydrates and are valued for their biocompatibility, biodegradability, and diverse biological activities. In this study, lactic acid bacteria were isolated...
Gene expression, enzyme purification and physicochemical characterization of Weissella confusa P2 glucansucrase [0.03%]
Weissella confusa P2葡糖焦磷酸酶的基因表达、酶的纯化及理化性质研究
Jinlong Wang,Jing Jiang,Gubo Gao et al.
Jinlong Wang et al.
In this study, the glucansucrase-encoding gene (dsr) from Weissella confusa P2 was cloned and heterologously expressed in Escherichia coli BL21(DE3). A 4542 bp fragment encoding 1510 amino acids was obtained, and the recombinant enzyme exhi...
Advances in modification strategies for polymer-based biocarriers in biofilm wastewater treatment [0.03%]
基于聚合物的生物载体改性策略在膜法废水处理中的应用进展
Dexin Su,Yupeng Chen
Dexin Su
Biofilm technology is an effective biological wastewater treatment method that has attracted significant attention. As the core component of this technology, biocarriers play a vital role in microorganism attachment and biofilm formation. P...
Integrated statistical and machine-learning optimization for enhanced heparosan production by Lactococcus lactis [0.03%]
集统计和机器学习优化于一体的N-乙酰氨基葡萄糖胺增强Lactococcus lactis高效产生海藻糖六硫酸盐的方法
Siddharth Guhan,Thilakraj S,Senthilkumar Sivaprakasam et al.
Siddharth Guhan et al.
Lactococcus lactis SH6 was engineered for heterologous heparosan production, and its growth medium was optimized using a combination of experimental design and machine learning (ML). One-factor-at-a-time shake flask experiments revealed glu...
Cost-effective production of vi polysaccharide through high-cell-density fermentation using animal component-free media for typhoid vaccines [0.03%]
一种成本效益高的动物成分无菌体多糖培养基，用于通过高细胞密度发酵生产伤寒疫苗中的Vi多糖蛋白质组分
Anirban Ray,Charles David,Pradeep Gupta et al.
Anirban Ray et al.
Salmonella enterica serovar Typhi remains a major public health concern that requires an effective strategy to produce vaccines. This study reports the development of a high cell density fermentation (HCD) process to increase the production...
Enhanced production of bacterial nanocellulose by Bacillus subtilis using combined mutagenesis and response surface optimization approaches [0.03%]
采用联合诱变和响应面优化方法提高枯草芽孢杆菌生产细菌纳米纤维素的产量
Lakshmi Gollapalli,Ranga Rao Ambati
Lakshmi Gollapalli
In the present study, a native Bacillus subtilis isolate-8 was subjected to sequential mutagenesis using ultraviolet (UV) irradiation, nitrous acid (HNO2) and N-methyl-N'-nitro-N-nitrosoguanidine (NTG) to obtain enhanced BNC production by f...
Plasmid design, cloning, and expression of Vitreoscilla hemoglobin in Streptomyces pilosus: a novel strategy to enhance desferrioxamine B production and yield [0.03%]
放线菌 pilosus 中 Vitreoscilla 血红蛋白的质粒设计、克隆和表达：一种提高脱铁氧霉素B产量的新策略
Mahsa Arkani,Valiollah Babaeipour,Ali Asghar Deldar et al.
Mahsa Arkani et al.
Streptomyces pilosus is the primary producer of desferrioxamine B (DFOB), to treat acute and chronic iron overload. Its growth and productivity depend on dissolved oxygen concentration. Vitreoscilla hemoglobin (VHb enhances oxygen transfer ...
Optimization of poly (3-hydroxybutyrate) production by recombinant Escherichia coli using an experimental screening design [0.03%]
采用实验筛选设计优化重组大肠杆菌中聚(3-羟基丁酸)的生产
Aleyna Filizfidanoglu,Tugbay-Sıla Altac,Yunus Ensari et al.
Aleyna Filizfidanoglu et al.
The negative effects of synthetic plastics on ecosystems and human health have increased interest in environmentally friendly and biodegradable bioplastics. Among bioplastics, polyhydroxyalkanoates (PHAs) stand out due to their biodegradabi...