Brandon J McKenna,J Herbert Waite,Galen D Stucky
Brandon J McKenna
Biomineralization is an intricate process that relies on precise physiological control of solution and interface properties. Despite much research of the process, mechanistic details of biomineralization are only beginning to be understood,...
Molecular Solids from Symmetrical Bis(piperazine-2,5-diones) with Open and Closed Monomer Conformations [0.03%]
具有开放式和闭合式单体构象的对称双（哌嗪-2,5-二酮）分子固态研究
Nathan W Polaske,Gary S Nichol,Lajos Z Szabó et al.
Nathan W Polaske et al.
The design, synthesis and solid state structures of a new class of xylylene-linked bis(1,4- piperazine-2,5-diones) are reported in an effort to extend the molecular framework of piperazine-2,5-diones. These compounds were derived from piper...
Seila Selimović,Yanwei Jia,Seth Fraden
Seila Selimović
We employ the PhaseChip, a (poly)dimethylsiloxane (PDMS) microfluidic device, for statistical studies of protein crystal nucleation. The PhaseChip is designed to decouple nucleation and growth of protein crystals and so improve their yield ...
Microfluidic Generation of Lipidic Mesophases for Membrane Protein Crystallization [0.03%]
用于膜蛋白结晶的液晶相微流体制备方法
Sarah L Perry,Griffin W Roberts,Joshua D Tice et al.
Sarah L Perry et al.
We report on a microfluidic method for the formation of aqueous/lipid mesophases to enable screening of suitable crystallization conditions of membrane proteins from a membrane-like phase in sub-20 nanoliter volumes. This integrated microfl...
Protein Crystal Engineering of YpAC-IV using the Strategy of Excess Charge Reduction [0.03%]
利用降低净电荷的策略进行YpAC-IV蛋白晶体工程改造
D Travis Gallagher,N Natasha Smith,Sook-Kyung Kim et al.
D Travis Gallagher et al.
The class IV adenylyl cyclase from Yersinia pestis has been engineered by site-specific mutagenesis to facilitate crystallization at neutral pH. The wild-type enzyme crystallized only below pH 5, consistent with the observation of a carboxy...
Supramolecular synthesis based on a combination of hydrogen- and halogen bonds [0.03%]
基于氢键和卤素键结合的超分子合成
Christer B Aakeröy,Nate Schultheiss,Arbin Rajbanshi et al.
Christer B Aakeröy et al.
A family of supramolecular reagents containing two different binding sites, pyridine and amino-pyrimidine, were allowed to react with iodo- or bromo-substituted benzoic acids in order to assemble individual molecules into larger architectur...
Organization of lower rim O-alkylated p-phosphonic acid calix[4]arenes [0.03%]
4-羟基-calix-[4]arene的下位环烷氧基化膦酸盐的组织结构式研究
Thomas E Clark,Mohamed Makha,Alexandre N Sobolev et al.
Thomas E Clark et al.
Lower rim O-methyl, -n-butyl, and n-octadecyl calix[4]arenes bearing p-phosphonic acid groups on the upper rim have been prepared in high yield, compounds 12-14. Where possible the compounds have been characterized in the solid state using ...
Function-biased choice of additives for optimization of protein crystallization - the case of the putative thioesterase PA5185 from Pseudomonas aeruginosa PAO1 [0.03%]
偏向功能的选择添加剂以优化蛋白质结晶-来自绿脓杆菌PA01的假定的硫酯酶PA5185的例子
Maksymilian Chruszcz,Matthew D Zimmerman,Shuren Wang et al.
Maksymilian Chruszcz et al.
The crystal structure of PA5185, a putative thioesterase from Pseudomonas aeruginosa strain PAO1, was solved using multi-wavelength anomalous diffraction to 2.4 Å. Analysis of the structure and information about the putative function of th...
Postcrystallization Analysis of the Irreproducibility of the Human Intrinsic Factor-Cobalamin Complex Crystals [0.03%]
人固有因子-维生素B12复合物晶体不可重复性的析晶后分析
N Sukumar,F S Mathews,M M Gordon et al.
N Sukumar et al.
Approximately 15% (w/w) of human intrinsic factor (IF) is comprised of carbohydrate side chains, making crystallization problematic. In addition, IF is sensitive to proteolysis. To understand the role of these factors in crystallization, we...
Atul S Deshpande,Elia Beniash
Atul S Deshpande
Mineralized collagen fibrils constitute a basic structural unit of collagenous mineralized tissues such as dentin and bone. Understanding of the mechanisms of collagen mineralization is vital for development of new materials for the hard ti...