Hierarchical Design Synergizing Local Dynamics Optimization and Global Rigidification Unlocks the Catalytic Potential of d-Erythrose-4-phosphate Dehydrogenase [0.03%]
层级设计协同局部动力学优化和全局刚性化解锁d-赤藓糖-4-磷酸脱氢酶的催化潜力
Chunxiang Pu,Tenghe Wang,Yu Lv et al.
Chunxiang Pu et al.
A key challenge in enzyme engineering is coordinating local catalytic dynamics with global structural integrity. Here, we present a hierarchical strategy integrating local dynamics optimization with global scaffold rigidification, using d-e...
Odorant-Binding Protein 18 Contributes to Tolerance-Related Responses to Flonicamid and Its Metabolite in Apis mellifera ligustica [0.03%]
意桑蜂Flonicamid及其代谢物耐受性相关响应中的OBP18作用机制研究
Peitong Chen,Ru Yan,Liangdong Wang et al.
Peitong Chen et al.
Honey bees rely on olfaction to perceive pheromones and environmental chemicals. Odorant-binding proteins (OBPs) are key carriers in odorant recognition, while growing studies suggest that OBPs may function beyond chemosensation, particular...
Multidimensional Metabolic Engineering for Green and Sustainable Bioproduction of Serotonin from Glucose in Bacillus licheniformis [0.03%]
用于从葡萄糖绿色可持续生物合成血清素的多维代谢工程研究
Xinyuan Yang,Yangyang Wang,Ji Li et al.
Xinyuan Yang et al.
Serotonin (5-hydroxytryptamine, 5-HT) plays a critical role in maintaining intestinal health, regulating mood and sleep, and enhancing crop stress tolerance. Here, a Bacillus licheniformis cell factory was developed for the sustainable and ...
The E3 Ubiquitin-Protein Ligase ZmBOIR3 Is a Key Regulator of Low-Temperature Tolerance in Maize Seedlings [0.03%]
E3泛素蛋白连接酶ZmBOIR3是玉米幼苗低温耐受性的关键调控因子
Qingyu Xu,Yingli Sun,Hongchen Du et al.
Qingyu Xu et al.
Low-temperature stress seriously affects the growth and development of maize seedlings, endangering their quality and yield. In this study, the low-temperature tolerance at the seedling stage of 299 maize inbred lines was evaluated by using...
ATP Synthase Subunit b Binds Cry2Ab and Mediates Its Toxicity in Grapholita molesta [0.03%]
线粒体ATP合成酶β亚基介导Cry2Ab毒素杀虫机理
Xiaoyan Yang,Yanshen Fu,Jiacheng Ye et al.
Xiaoyan Yang et al.
The oriental fruit moth (Grapholita molesta), a worldwide fruit tree pest, can be effectively controlled by Bacillus thuringiensis (Bt), with the insect peritrophic membrane (PM) serving as a key target for such control. This study investig...
Daurichromenic Acid Isolated from Rhododendron rupicola var. chryseum Exhibits Antibacterial Activity against Xanthomonas citri subsp. citri [0.03%]
Rhododendron rupicola var. chryseum中分离的Daurichromenic酸对柑橘疫病菌Xanthomonas citri subsp. citri具有抗菌活性
Jia Zhang,Ai-Chun Lu,Yan Liang et al.
Jia Zhang et al.
Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is a devastating bacterial disease. Given the resistance and pollution from long-term chemical pesticides, plant-derived natural products offer green alternatives. Here, daurich...
Vibration-Induced Texture Deterioration in Kiwifruit: Molecular Mechanisms and Modulation by 1-MCP-Mediated Pectin Stabilization [0.03%]
振动引起的奇异果质地劣变的分子机制及1-MCP介导的pectin稳定化调控研究
Haishan Xu,Yiyang Li,Haiying Yang et al.
Haishan Xu et al.
Vibration stress during transportation represents a mechanical factor that accelerates texture deterioration. This study revealed the molecular basis underlying vibration-induced texture deterioration in kiwifruit and further evaluated 1-me...
Copper Exposure Promotes Mitochondrial VDAC Oligomerization and Releases mtDNA to Induce Pyroptosis in Pig Hepatocytes [0.03%]
铜暴露通过促进线粒体孔蛋白寡聚化释放mtDNA诱导猪肝细胞吡啶虫素凋亡
Wenyue Qiu,Wei Chen,Shanglong Yang et al.
Wenyue Qiu et al.
Copper (Cu) is widely used as a growth-promoting trace element in swine feed, but excessive Cu causes liver toxicity and modulates innate immunity, with unclear molecular mechanisms. In this study, dietary Cu overload in pigs led to Cu accu...
Controlled Trypsin Hydrolysis of Bovine Casein for Preferential αS1-Casein Reduction: Fraction-Specific Kinetics, αS1-Casein-Related Antigenicity and Candidate Bioactive Peptides [0.03%]
调控胰蛋白酶水解牛酪蛋白以优先减少αS1-酪蛋白：组分特异性动力学，αS1-酪蛋白相关抗原性和候选生物活性肽
Bao-Rong Wang,Stella Zhu,Wen-Qi Liang et al.
Bao-Rong Wang et al.
Cow's milk-based infant formula aims to approximate human milk, but bovine αS1-casein remains an important allergen-related protein with a profile distinct from human milk caseins. This study controlled trypsin hydrolysis to preferentially...
Butyrate-Dependent Conformational Switch Promotes Nuclear Translocation of the Mustard Allergen Sin a 1 in Human Gut Epithelial Cells [0.03%]
丁酸依赖的构象转换促进芥子过敏原Sin a 1在人肠道上皮细胞中进入细胞核转运
Rubén G Gordo,Jorge Parrón-Ballesteros,Nieves Olmo et al.
Rubén G Gordo et al.
Sin a 1, the major allergen from mustard seeds, is a highly stable 2S albumin capable of reaching the intestinal epithelium in its intact form. In this study, we investigated whether butyrate, a microbiota-derived short-chain fatty acid, di...