Enhanced extracellular production of raw starch-degrading α-amylase in Bacillus subtilis through expression regulatory element modification and fermentation optimization [0.03%]
通过表达调控元件修改和发酵优化在枯草芽孢杆菌中增强α-淀粉酶的胞外合成能力
Dongbang Yao,Xudong Han,Huanhuan Gao et al.
Dongbang Yao et al.
Background: Raw starch-degrading α-amylase (RSDA) can hydrolyze raw starch at moderate temperatures, thus contributing to savings in starch processing costs. However, the low production level of RSDA limits its industria...
High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux [0.03%]
高通量¹³C-代谢流分析表明，丙酰辅酶A和ATP由于糖酵解流的严格控制而有限导致限制了3-羟基丙酸生产菌毕赤氏酵母中的供给
Albert Fina,Pierre Millard,Joan Albiol et al.
Albert Fina et al.
Background: Production of 3-hydroxypropionic acid (3-HP) through the malonyl-CoA pathway has yielded promising results in Pichia pastoris (Komagataella phaffii), demonstrating the potential of this cell factory to produce...
Characterization of 17β-estradiol-degrading enzyme from Microbacterium sp. MZT7 and its function on E2 biodegradation in wastewater [0.03%]
来源于Microbacterium sp. MZT7的雌二醇降解酶的性质鉴定及其在废水中的功能研究
Peng Hao,Hanyu Pan,Zongshuo Lv et al.
Peng Hao et al.
Background: 17β-estradiol (E2) residues exhibit harmful effects both for human and animals and have got global attention of the scientific community. Microbial enzymes are considered as one of the effective strategies ha...
Metabolic engineering of Escherichia coli for high-level production of violaxanthin [0.03%]
用于高水平合成新黄素的大肠杆菌代谢工程菌株的构建及发酵优化
Dong Xinrui,Liu Bo,Bao Yihong et al.
Dong Xinrui et al.
Background: Xanthophylls are a large class of carotenoids that are found in a variety of organisms and play particularly important roles in the light-harvesting and photoprotection processes of plants and algae. Violaxant...
Insight into broad substrate specificity and synergistic contribution of a fungal α-glucosidase in Chinese Nong-flavor daqu [0.03%]
中国浓香型大曲中半纤维素降解真菌α-葡萄糖苷酶的底物普适性及协同贡献机制研究
Zhuolin Yi,Lanchai Chen,Yanling Jin et al.
Zhuolin Yi et al.
Background: Chinese Nong-favor daqu, the presentative liquor starter of Baijiu, has been enriched with huge amounts of enzymes in degrading various biological macromolecules by openly man-made process for thousand years. ...
Engineering of cyclodextrin glycosyltransferase improves the conversion efficiency of rebaudioside A to glucosylated steviol glycosides and increases the content of short-chain glycosylated steviol glycoside [0.03%]
工程化环糊精葡萄糖转移酶可提高甜菊糖苷A转化成葡糖基甘草次酸甘苷的效率并增加短链葡糖基甘草次酸甘苷含量
Ruiqin Zhang,Ruiqi Tang,Wei Wang et al.
Ruiqin Zhang et al.
Background: Compared with steviol glycosides, the taste of glucosylated steviol glycosides is better and more similar to that of sucrose. At present, cyclodextrin glucanotransferase (CGTase) is primarily used to catalyze ...
Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668 [0.03%]
枯草芽孢杆菌AR668中CRISPR-Cas9系统的建立
Jiao Li,Xin Song,Zhiqiang Xiong et al.
Jiao Li et al.
Bifidobacteria are representative intestinal probiotics that have extremely high application value in the food and medical fields. However, the lack of molecular biology tools limits the research on functional genes and mechanisms of bifido...
Lactiplantibacillus plantarum: a new example of inclusion body producing bacteria [0.03%]
植物乳杆菌：细菌产生包涵体的新例证
Ricardo Baltà-Foix,Caterina Serrano-Adrover,Adrià López-Cano et al.
Ricardo Baltà-Foix et al.
Background: Lactic Acid Bacteria such as Lactococcus lactis, Latilactobacillus sakei (basonym: Lactobacillus sakei) and Lactiplantibacillus plantarum (basonym: Lactobacillus plantarum) have gained importance as recombinan...
Pseudomonas aeruginosa's greenish-blue pigment pyocyanin: its production and biological activities [0.03%]
绿脓杆菌绿色蓝色色素吡ocrassin的产生及生物学活性
Ahmed A Abdelaziz,Amal M Abo Kamer,Khaled B Al-Monofy et al.
Ahmed A Abdelaziz et al.
A subject of great interest is the bioprospecting of microorganisms and their bioactive byproducts, such as pigments. Microbial pigments have various benefits, including being safe to use due to their natural makeup, having therapeutic effe...
Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach [0.03%]
利用多因素工程技术改善益生酵母Saccharomyces boulardii中治疗蛋白的分泌
Deniz Durmusoglu,Ibrahim Al&#x;Abri,Zidan Li et al.
Deniz Durmusoglu et al.
The probiotic yeast Saccharomyces boulardii (Sb) is a promising chassis to deliver therapeutic proteins to the gut due to Sb's innate therapeutic properties, resistance to phage and antibiotics, and high protein secretion capacity. To maint...