Optimization of hydrogen production in Enterobacter aerogenes by Complex I peripheral fragments destruction and maeA overexpression [0.03%]
通过破坏复杂I周边片段和过表达maeA来优化肠杆菌产氢性能
Ke Jiang,Ruoxuan Bai,Ting Gao et al.
Ke Jiang et al.
As a concentrated energy source with high added value, hydrogen has great development prospects, with special emphasis on sustainable microbial production as a replacement for traditional fossil fuels. In this study, λ-Red recombination wa...
A novel strategy for L-arginine production in engineered Escherichia coli [0.03%]
一种在工程菌E.coli中产生L-精氨酸的新策略
Mengzhen Nie,Jingyu Wang,Kechun Zhang
Mengzhen Nie
Background: L-arginine is an important amino acid with applications in diverse industrial and pharmaceutical fields. N-acetylglutamate, synthesized from L-glutamate and acetyl-CoA, is a precursor of the L-arginine biosynt...
Filter cake extract from the beet sugar industry as an economic growth medium for the production of Spirulina platensis as a microbial cell factory for protein [0.03%]
糖蜜滤泥提取物在经济型培养基中的应用及其用于生产螺旋藻的细胞工厂作用
Sara Saad,Mervat Hosny Hussien,Ghada Samir Abou-ElWafa et al.
Sara Saad et al.
Background: Beet filter cake (BFC) is a by-product of sugar beet processing, which is difficult to dispose of and involves severe environmental concerns. Spirulina platensis is a microalga with a high protein content esse...
Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions [0.03%]
甲醇和无甲醇培养条件下通过共表达辅助蛋白优化毕赤酵母中国纳单体的表达
Manu De Groeve,Bram Laukens,Peter Schotte
Manu De Groeve
Background: Ablynx NV, a subsidiary of Sanofi, has a long-standing focus on the development of Nanobody® molecules as biopharmaceuticals (Nanobody® is a registered trademark of Ablynx NV). Nanobody molecules are single ...
Flavour by design: food-grade lactic acid bacteria improve the volatile aroma spectrum of oat milk, sunflower seed milk, pea milk, and faba milk towards improved flavour and sensory perception [0.03%]
风味设计：食品级乳酸菌改善燕麦奶、向日葵籽奶、豌豆奶和蚕豆奶的挥发性香气谱，提升风味和感官体验
Muzi Tangyu,Michel Fritz,Jan Patrick Tan et al.
Muzi Tangyu et al.
Background: The global market of plant-based milk alternatives is continually growing. Flavour and taste have a key impact on consumers' selection of plant-based beverages. Unfortunately, natural plant milks have only lim...
Promising approaches for the assembly of the catalytically active, recombinant Desulfomicrobium baculatum hydrogenase with substitutions at the active site [0.03%]
组装具有活性中心突变的催化性重组脱硫微球菌氢酶的前景展望
Malgorzata Witkowska,Robert P Jedrzejczak,Andrzej Joachimiak et al.
Malgorzata Witkowska et al.
Background: Hydrogenases (H2ases) are metalloenzymes capable of the reversible conversion of protons and electrons to molecular hydrogen. Exploiting the unique enzymatic activity of H2ases can lead to advancements in the ...
Efficient production of bacterial antibiotics aminoriboflavin and roseoflavin in eukaryotic microorganisms, yeasts [0.03%]
棒状杆菌抗生素氨小孢子菌素和玫瑰红霉素的高效生产系利用酵母等真核微生物体系实现
Kostyantyn V Dmytruk,Justyna Ruchala,Liubov R Fayura et al.
Kostyantyn V Dmytruk et al.
Background: Actinomycetes Streptomyces davaonensis and Streptomyces cinnabarinus synthesize a promising broad-spectrum antibiotic roseoflavin, with its synthesis starting from flavin mononucleotide and proceeding through ...
Cell-growth phase-dependent promoter replacement approach for improved poly(lactate-co-3-hydroxybutyrate) production in Escherichia coli [0.03%]
基于细胞生长周期的启动子替换提高大肠杆菌中聚乳酸-共-3-羟基丁酸酯积累水平的新方法
Yuki Nagao,Sangho Koh,Seiichi Taguchi et al.
Yuki Nagao et al.
Escherichia coli is a useful platform for producing valuable materials through the implementation of synthetic gene(s) derived from other organisms. The production of lactate (LA)-based polyester poly[LA-co-3-hydroxybutyrate (3HB)] was carr...
From frozen cell bank to product assay: high-throughput strain characterisation for autonomous Design-Build-Test-Learn cycles [0.03%]
从冷冻细胞库到产品检测：用于自主“设计-构建-测试-学习”循环的高通量菌株表征
Laura M Helleckes,Debora Puchta,Hannah Czech et al.
Laura M Helleckes et al.
Background: Modern genome editing enables rapid construction of genetic variants, which are further developed in Design-Build-Test-Learn cycles. To operate such cycles in high throughput, fully automated screening, includ...
Polyhydroxybutyrate production by recombinant Escherichia coli based on genes related to synthesis pathway of PHB from Massilia sp. UMI-21 [0.03%]
基于Massilia sp. UMI-21聚β-羟基丁酸酯合成途径相关基因的工程菌聚乳酸生产性能研究
Nan Jiang,Ming Wang,Linxin Song et al.
Nan Jiang et al.
Background: Polyhydroxybutyrate (PHB) is currently the most common polymer produced by natural bacteria and alternative to conventional petrochemical-based plastics due to its similar material properties and biodegradabil...