A temperature dependent pilin promoter for production of thermostable enzymes in Thermus thermophilus [0.03%]
一种温度依赖型pilin启动子在嗜热栖热菌中生产耐热酶中的应用
Lennart Kirchner,Volker Müller,Beate Averhoff
Lennart Kirchner
Background: Enzymes from thermophiles are of great interest for research and bioengineering due to their stability and efficiency. Thermophilic expression hosts such as Thermus thermophilus [T. thermophilus] can overcome ...
Engineering Comamonas testosteroni for the production of 2-pyrone-4,6-dicarboxylic acid as a promising building block [0.03%]
工程菌康氏耐盐假单胞菌用于生产具有前景的砌块化合物2-吡喃酮-4,6-二羧酸
Tom Delmulle,Stijn Bovijn,Sari Deketelaere et al.
Tom Delmulle et al.
Background: Plastics are an indispensable part of our daily life. However, mismanagement at their end-of-life results in severe environmental consequences. The microbial conversion of these polymers into new value-added p...
A3DyDB: exploring structural aggregation propensities in the yeast proteome [0.03%]
A3DyDB：探索酵母蛋白质组中的结构聚集倾向性
Javier Garcia-Pardo,Aleksandra E Badaczewska-Dawid,Carlos Pintado-Grima et al.
Javier Garcia-Pardo et al.
Background: The budding yeast Saccharomyces cerevisiae (S. cerevisiae) is a well-established model system for studying protein aggregation due to the conservation of essential cellular structures and pathways found across...
Construction and testing of Yarrowia lipolytica recombinant protein expression chassis cells based on the high-throughput screening and secretome [0.03%]
基于高通量筛选和分泌组的解脂耶罗华酵母重组蛋白表达底盘细胞的构建与验证
Siqian Yu,Ge Zhang,Qi Liu et al.
Siqian Yu et al.
Background: In the recombinant protein market with broad economic value, the rapid development of synthetic biology has made it necessary to construct an efficient exocrine expression system for the different heterologous...
High-throughput process development from gene cloning to protein production [0.03%]
从基因克隆到蛋白质生产的高通量工艺开发
Manman Sun,Alex Xiong Gao,Xiuxia Liu et al.
Manman Sun et al.
In the post-genomic era, the demand for faster and more efficient protein production has increased, both in public laboratories and industry. In addition, with the expansion of protein sequences in databases, the range of possible enzymes o...
Generation of green electricity from sludge using photo-stimulated bacterial consortium as a sustainable technology [0.03%]
一种可持续技术：利用光刺激菌群丛经济高效地从污泥中发电
Amal S Othman,Nashwa A Ahmed,Mona S Elneklawi et al.
Amal S Othman et al.
Microbial fuel cell (MFC) is a bio-electrical energy generator that uses respiring microbes to transform organic matter present in sludge into electrical energy. The primary goal of this work was to introduce a new approach to the green ele...
A strategy to promote the convenient storage and direct use of polyhydroxybutyrate-degrading Bacillus sp. JY14 by lyophilization with protective reagents [0.03%]
冻干保护剂筛选及芽孢杆菌JY14的保存研究策略
Su Hyun Kim,Nara Shin,Suk Jin Oh et al.
Su Hyun Kim et al.
Background: Bioplastics are attracting considerable attention, owing to the increase in non-degradable waste. Using microorganisms to degrade bioplastics is a promising strategy for reducing non-degradable plastic waste. ...
Economical synthesis of γ-cyclodextrin catalyzed by oriented cyclodextrin glycosyltransferase displayed on bacterial polyhydroxyalkanoate nanogranules [0.03%]
固定化环糊精葡萄糖转移酶定向催化合成γ-环糊精的高效方法研究
Menglu Duan,Yan Wang,Dan Tan et al.
Menglu Duan et al.
Background: The advantages of γ-cyclodextrin (γ-CD) include its high solubility, ability to form inclusion complexes with various poorly water-soluble molecules, and favorable toxicological profile; thus, γ-CD is an at...
Analysis of acid-tolerance mechanism based on membrane microdomains in Saccharomyces cerevisiae [0.03%]
基于膜微区的酵母耐酸机制分析研究
Xueqin Lv,Ke Jin,Yu Yi et al.
Xueqin Lv et al.
Background: Saccharomyces cerevisiae has been used in the biosynthesis of acid products such as organic acids owing to its acid tolerance. Improving the acid tolerance of S. cerevisiae is beneficial for expanding its appl...
Cost-effective production of alginate oligosaccharides from Laminaria japonica roots by Pseudoalteromonas agarivorans A3 [0.03%]
利用海洋假交替单胞菌A3高效低成本地从海带根中生产岩藻糖寡糖
Xiao-Hui Sun,Xiu-Lan Chen,Xiao-Fei Wang et al.
Xiao-Hui Sun et al.
Background: Alginate oligosaccharides (AOs) are the degradation products of alginate, a natural polysaccharide abundant in brown algae. AOs generated by enzymatic hydrolysis have diverse bioactivities and show broad appli...