Rewiring central metabolism in Komagataella phaffii for efficient mannose synthesis [0.03%]
通过改造中心代谢途径高效地在毕赤酵母中合成甘露糖
Sijia Zhao,Yuheng Wang,Qian Li et al.
Sijia Zhao et al.
Background: Mannose has wide-ranging applications but microbial fermentation remains underdeveloped compared to biotransformation for its production. The yeast Komagataella phaffii stands out as a premier synthetic biolog...
Application of vibrational spectroscopies as process analytical techniques for monitoring fermentation and the conversion of lignocellulosic biomass by oleaginous filamentous fungi [0.03%]
振动光谱技术在发酵过程中监测以及丝状酵母将木质纤维素生物量转化过程中的应用
Simona Dzurendova,Cristian Bolaño Losada,Benjamin Xavier Dupuy-Galet et al.
Simona Dzurendova et al.
Background: Oleaginous filamentous fungi, such as Mucor circinelloides, are capable of accumulating high levels of single cell oil (SCO), making them attractive candidates for the production of biodiesel and other oleoche...
Investigating the probiotic and flavor-producing functions of Bacillus sp. FPIK1 sourced from fermented bamboo shoots of Assam [0.03%]
来自阿萨姆发酵竹笋的芽孢杆菌FPIK1的益生菌和产香功能研究
Debashree Borthakur,Bipin Kumar Sharma,Twesigye Duncan
Debashree Borthakur
Background: Fermented bamboo shoots, locally known as Khorisa, are a traditional food widely consumed in Assam, India, valued for their distinctive tangy flavour and potential probiotic benefits. This study aimed to isola...
Efficient refolding, purification, and characterization of barley oxalate oxidase in Escherichia coli [0.03%]
大肠杆菌中草酸氧化酶的高效复性、纯化及鉴定
William Donelan,ShiWu Li,Paul R Dominguez-Gutierrez et al.
William Donelan et al.
Background: Oxalate oxidase (OxOx) is an important enzyme commonly used in the determination of urine oxalate concentration. It is naturally expressed by a variety of plants and microorganisms and recombinant OxOx enzyme ...
Recombinant MAM from Faecalibacterium duncaniae exhibits a protective effect in DNBS-induced colitis [0.03%]
弗氏菌属邓氏菌株的MAM重组蛋白对DNBS诱导结肠炎具有保护作用
Thaís Vilela Rodrigues,Luís Lima de Jesus,Monique Ferrary Américo et al.
Thaís Vilela Rodrigues et al.
Background: Microbial anti-inflammatory molecule (MAM) is a key effector of the next-generation probiotic Faecalibacterium duncaniae A2-165, a species whose depletion in the gut microbiota is strongly linked to inflammato...
High hydrostatic pressure enhances chitinase activity and reveals metabolic regulation via transcriptomics in an abyssal fungus Purpureocillium lilacinum FDZ8Y1 [0.03%]
高压促进深渊真菌Purpureocillium lilacinum FDZ8Y1中几丁质酶活性并揭示转录组学介导的代谢调控
Shenao Hu,Yongqi Li,Shicong Wan et al.
Shenao Hu et al.
Background: The abyssal environment is characterized by extreme conditions such as high hydrostatic pressure (HHP), low temperature, and high salinity, which affects the protein synthesis of filamentous fungi. The targete...
Metabolic engineering of Phaeodactylum tricornutum for heterologous production of the plant triterpenoid friedelin [0.03%]
角毛藻代谢工程异源生产植物三萜friedelin
Khanh Nguyen,Min Sun Choi,Seungbeom Seo et al.
Khanh Nguyen et al.
Background: Friedelin is a pharmacologically valuable pentacyclic triterpenoid with anti-inflammatory, anticancer, antiviral, and antiobesity properties. Conventional methods of friedelin production rely on solvent-intens...
Cell surface display of VP1 of foot-and-mouth disease virus on Saccharomyces cerevisiae [0.03%]
用于酿酒酵母细胞表面展示的口蹄疫病毒VP1蛋白及其融合基因和重组菌株
Ngoc My Tieu Le,Jeesun Chun,Yo-Han Ko et al.
Ngoc My Tieu Le et al.
Background: Foot-and-mouth disease (FMD) is a highly infectious disease that mainly affects cloven-hoofed animals. The rapid spread of this disease hinders the success of control measures. This necessitates the urgent dev...
Multi-omics analysis reveals the carbohydrate-active enzyme repertoire involved in degrading complex arabinoxylans in Penicillium parvum 4-14 [0.03%]
多组学分析揭示了参与少孢青霉4-14降解复杂阿拉伯木聚糖的碳水化合物活性酶谱系
Liangkun Long,Jiarong Zhu,Yijia Teng et al.
Liangkun Long et al.
Background: Corn bran arabinoxylan (CBAX) is one of the most structurally complex xylans in nature. The bioconversion of CBAX into value-added products remains challenging because the substrate is resistant to pure xylana...
Engineering of FK520 polyketide synthase for rapid access to quality control reference standards [0.03%]
工程改造FK520聚酮合成酶快速获得质量控制标准品
Nina Žigart,Petra Pivk Lukančič,Tjaša Drčar et al.
Nina Žigart et al.
Despite the ability of mega synthases to construct complex structures with high precision, the main product is often accompanied by minor congeners because of relaxed selectivity of biosynthetic steps. In the case of polyketides, these may ...