Filamentous multiscale characterization of the cultivation of Actinomadura namibiensis pellets for model-based prediction of labyrinthopeptin A1 biosynthesis [0.03%]
放线菌聚集体的丝状多尺度表征及其用于迷宫环肽A1生物合成预测模型的基础研究
Qiyue Liu,Zuzanna Justyna Kozanecka,Jintian Liu et al.
Qiyue Liu et al.
The filamentous actinomycete Actinomadura namibiensis has attracted increasing interest as the only known natural producer of the carbocyclic lantibiotic labyrinthopeptin A1, a secondary metabolite with a broad antiviral spectrum of activit...
Adaptive laboratory evolution of Saccharomyces cerevisiae enhances butyric acid tolerance and enables co-cultivation with Clostridium tyrobutyricum [0.03%]
模式酵母酿酒酵母的适应性实验室进化提高了丁酸耐受性并使其与丁酸梭菌共培养成为可能
K Oehlenschläger,S Bauschatz,E Schepp et al.
K Oehlenschläger et al.
Adaptive laboratory evolution (ALE) was successfully applied to improve the tolerance of Saccharomyces cerevisiae toward butyric acid, enabling its use in co-culture with Clostridium tyrobutyricum for the simultaneous production of ethanol ...
Fine-tuned synthetic transcription factors for production of 3'-phosphoadenosine-5'-phosphosulfate in yeast [0.03%]
用于酵母中产生3'-腺苷5'-磷硫酸的精细调节合成转录因子
Madhushruti Borah,Shanna Gu,Essa M Saied et al.
Madhushruti Borah et al.
Background: Technologies developed over the past decade have made Saccharomyces cerevisiae a promising platform for producing various natural products. Balancing multi-enzyme expression, while maintaining robust microbial...
Transcriptional reprogramming of the stress response and translational-secretory machinery enhances recombinant secreted protein production in Yarrowia lipolytica [0.03%]
解脂耶罗孢膜酵母转录和翻译分泌机制的重编程强化重组蛋白的生产
Maria Gorczyca,Piotr Kubiak,Ewelina Celińska
Maria Gorczyca
Protein synthesis and secretion processes are non-pathway traits. The genetic complexity makes them very challenging to engineer and genetically optimize. Therefore, we exploit transcriptional reprogramming to create a favorable cellular en...
A versatile toolset for Staphylococcus aureus gene inactivation enables testing of anti-phage defense systems in native genetic backgrounds [0.03%]
一个用于金黄色葡萄球菌基因失活的多功能工具组可在天然遗传背景下检测噬菌体防御系统
Łukasz Kałuski,Stephen Amankwah,Laura Wicke et al.
Łukasz Kałuski et al.
Background: Functional gene analysis of Staphylococcus aureus across different strains requires the development of genetic engineering tools. In studies on phage therapy, such analysis is especially important for understa...
Biocatalytic synthesis of alkoxylated phenazine derivatives using methyltransferases [0.03%]
甲基转移酶催化合成茋衍生物茋茋骨架的烷氧基化酚嗪衍生物的生物催化合成
Chaozhi Wang,Shuo Zhang,Sijia Xu et al.
Chaozhi Wang et al.
Background: Phenazines are important nitrogen-containing heterocycles with diverse applications in the chemical and pharmaceutical industry. Alkoxylated phenazines, in particular, exhibit promising acaricidal and fungicid...
Functional redundancy driven by isocitrate dehydrogenase enhances metabolic robustness in Pseudomonas putida KT2440 [0.03%]
鼠伤寒沙门氏菌KT2440中由异柠檬酸脱氢酶驱动的功能冗余增强了代谢稳健性
Maria-Tsampika Manoli,Sandra Herrera Alarcon,Álvaro Gargantilla-Becerra et al.
Maria-Tsampika Manoli et al.
Background: Prokaryotic isocitrate dehydrogenase (PIDH) is a conserved and highly versatile enzyme regulating carbon flux between the TCA cycle and the glyoxylate shunt. While most mesophilic organisms encode a single PID...
Enhancing recombinant eGFP secretion in P. tricornutum by optimizing culture conditions [0.03%]
通过优化培养条件增强P.tricornutum中重组eGFP的分泌
Nesrine Gargouch,Aurélie Charrier,Charlotte Toustou et al.
Nesrine Gargouch et al.
Phaeodactylum tricornutum is a promising candidate for the production of recombinant therapeutic proteins including antibodies. So far, low secretion yields remain a significant bottleneck for pharmaceutical applications. Towards the object...
Establishment of SRLC: a multiplex genome editing technology for Saccharomyces cerevisiae and its application in metabolic engineering of malonyl-CoA pathway [0.03%]
SRLC的建立及其在丙二酰辅酶A途径代谢工程中的应用
Wentao Zheng,Miao Wang,Qiang Tu et al.
Wentao Zheng et al.
The development of advanced genome engineering tools is crucial for optimizing metabolic pathways in Saccharomyces cerevisiae and achieving efficient biomanufacturing. This study proposes an enhancing multiplex genome editing strategy in S....
First evidence of keratinolytic activity in Simplicillium aogashimaense isolated from wool-pellet-amended soil [0.03%]
来源于羊毛粒改良土壤的Simplicillium aogashimaense菌株的角蛋白酶解活性的首个证据
Clémentine Isembart,Zoé Bruchon,Jean-Michel Yao et al.
Clémentine Isembart et al.
Background: Keratin-rich waste such as wool and feather wastes are generated in large quantities worldwide and remain difficult to valorize due to the structural resilience of keratin, that is reinforced by disulfide bond...