Read-mapping signatures for molecular characterization of transgenic DNA insertions in paired-end Illumina data [0.03%]
配对末端Illumina数据中转基因DNA插入位点的分子特征的确立及其在比对中的标志
Colton R Kessenich,Nicole Catherall,Elisa Hughes et al.
Colton R Kessenich et al.
Transgenic crops undergo rigorous safety assessments prior to commercialization, with molecular characterization serving as a critical component of regulatory review. This process establishes the identity, copy number, sequence integrity, a...
Comparative analysis of MinION and MiSeq using 16S rRNA gene amplicon sequencing in human gut microbiome [0.03%]
运用扩增子测序比较分析MinION和MiSeq在人体肠道微生物组中的表现
Chong Zhu,Tao Wang,Wenbo Zhang et al.
Chong Zhu et al.
The genetic classification of microbial populations at high taxonomic resolution is crucial for clinical diagnosis and treatment. The classification of complex bacterial communities in the human gut was examined in this study by utilizing a...
Integrated structure-based and systems-level identification of PDE9A inhibitors for heart failure with preserved ejection fraction [0.03%]
基于结构和系统水平的整合鉴定PDE9A抑制剂以治疗射血分数保留的心力衰竭
Xiaoyan Lei,Rujie Wang,Yongmei Li
Xiaoyan Lei
Background: Heart failure with preserved ejection fraction (HFpEF) is a complex and increasingly prevalent cardiovascular disorder with limited effective therapeutic options. Dysregulation of cyclic guanosine monophosphat...
Multi-omic characterization of a soil microbial consortium reveals critical role of succinate and glutamate metabolism during calcium carbonate precipitation [0.03%]
土壤微生物菌群的多组学表征揭示了在碳酸钙沉淀过程中琥珀酸和谷氨酸代谢发挥关键作用
Winston E Anthony,Tesia T Lin,Marci Garcia et al.
Winston E Anthony et al.
Microbially induced calcium carbonate precipitation (MICP) holds potential for soil stabilization and carbon sequestration efforts, with the overall efficiency of the process being a major determinant for its use in many environmental and c...
Integrative analysis identifies the intratumoral Fusobacterium nucleatum as a driver of cervical cancer malignancy [0.03%]
整合分析鉴定出宫颈部肿瘤内的福斯杆菌作为恶性转化驱动因素
Mei Feng,Yaojie Yu,Jiayuan Du et al.
Mei Feng et al.
Background: Fusobacterium nucleatum (F. nucleatum) is an oral commensal bacterium that acts as a pathobiont with pro-tumorigenic activity in various gastrointestinal cancers. However, its functional role, invasive capacit...
Production and partial characterization of fructose-rich exopolysaccharide from a newly isolated halophilic strain Vreelandella sp. DT-Z4 [0.03%]
来源于新分离的盐嗜菌株Vreelandella sp. DT-Z4的富含果糖的胞外多糖的生产及其部分性质分析
Fengqian Yang,Fangyan Wang,Chengyang Wang et al.
Fengqian Yang et al.
Exopolysaccharide (EPS) synthesis of halophilic microorganisms is often reported as an adaptation to high-salt environments, and the unique structural and functional characteristics of these EPSs make them valuable biomaterials with promisi...
Improving xylitol production by semi-rational engineering of xylitol dehydrogenase and optimizing cofactor regeneration in Gluconobacter oxydans [0.03%]
通过半理性工程化改造木糖醇脱氢酶及优化辅因子再生提高葡萄糖醋杆菌木糖醇产量
Jun-Hao Su,Jin-Cheng Guo,Lu Liu et al.
Jun-Hao Su et al.
Background: Xylitol, a widely used sugar substitute in food and medicine, can be produced through microbial bioconversion using glucose as the primary substrate. In this process, a critical factor limiting xylitol product...
Characterization of an alkali- and glucose-tolerant β-glucosidase from Karamay saline-alkali soil and its structural basis for glucose tolerance [0.03%]
一株来源于克拉玛依盐碱土的耐碱和耐葡萄糖β-葡萄糖苷酶菌株及其耐葡萄糖作用机制研究
Qian Zhu,Qin Duan,Fei Wang et al.
Qian Zhu et al.
Background: Industrial applications of β-glucosidases are often constrained by high salinity, alkaline conditions, and glucose inhibition. Results: ...
3D-printed metamaterial femoral prostheses via scalar field fusion and directional porous structure regulation [0.03%]
基于标量场融合和方向多孔结构调控的3D打印代谢物股骨假体
Guoqing Zhang,Xiaoyu Zhou,Junxin Li et al.
Guoqing Zhang et al.
Background: This study integrates topology optimization, scalar field fusion (a method to realize smooth transition between solid and porous regions through implicit function interpolation), and directional porous structu...
Characterization of novel cellobiose 2-epimerases from Teredinibacter Haidensis and Cellvibrio Japonicus [0.03%]
来源于Teredinibacter haidensis和Cellvibrio japonicus的新型半乳双糖2-差向异构酶的性质研究
Yaxian Liu,Sabine Lutz-Wahl,Lutz Fischer
Yaxian Liu
Background: Cellobiose 2-epimerases (CEs) are promising enzymes that catalyze the conversion of lactose to the bioactive disaccharides epilactose and lactulose. Low-temperature (≤8 °C) lactose bioconversion is essential...