Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris [0.03%]
用于毕赤酵母中植物生长素表达的联合策略优化研究
Cheng Li,Ying Lin,Xueyun Zheng et al.
Cheng Li et al.
Background: Phytase is used as an animal feed additive that degrades phytic acid and reduces feeding costs and pollution caused by fecal excretion of phosphorus. Some phytases have been expressed in Pichia pastoris, among...
Isolation of nanomolar scFvs of non-human primate origin, cross-neutralizing botulinum neurotoxins A1 and A2 by targeting their heavy chain [0.03%]
通过靶向其重链从非人灵长类动物中分离出纳摩尔级的单域抗体片段(scFv)，以实现A1和A2型肉毒神经毒素的交叉中和作用
Arnaud Avril,Sebastian Miethe,Michel R Popoff et al.
Arnaud Avril et al.
Background: Botulism is a naturally occurring disease, mainly caused by the ingestion of food contaminated by the botulinum neurotoxins (BoNTs). Botulinum neurotoxins are the most lethal. They are classified among the six...
Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells [0.03%]
来源于BHK、CHO和HEK293细胞的重组人凝血因子VII的不同N-糖基化形式
Ernst Böhm,Birgit K Seyfried,Michael Dockal et al.
Ernst Böhm et al.
BACKGROUND & Methods: Recombinant factor VII (rFVII), the precursor molecule for recombinant activated FVII (rFVIIa), is, due to its need for complex post...
Construction of polyomavirus-derived pseudotype virus-like particles displaying a functionally active neutralizing antibody against hepatitis B virus surface antigen [0.03%]
基于多瘤病毒的假病毒样颗粒展示对乙型肝炎病毒表面抗原中和抗体的功能活性
Milda Pleckaityte,Corinna M Bremer,Alma Gedvilaite et al.
Milda Pleckaityte et al.
Background: Virus-like particles (VLPs) can be efficiently produced by heterologous expression of viral structural proteins in yeast. Due to their repetitive structure, VLPs are extensively used for protein engineering an...
High-level expression of a recombinant active microbial transglutaminase in Escherichia coli [0.03%]
一种活性微生物转谷氨酰胺酶重组蛋白在大肠杆菌中的高效表达
Barbara Salis,Gaia Spinetti,Silvia Scaramuzza et al.
Barbara Salis et al.
Background: Bacterial transglutaminases are increasingly required as industrial reagents for in vitro modification of proteins in different fields such as in food processing as well as for enzymatic site-specific covalent...
Specific detection of foot-and-mouth disease serotype Asia 1 virus by carboxyl-magnetic beads conjugated with single-domain antibody [0.03%]
羧基磁珠结合单域抗体特异性检测口蹄疫A型病毒
Shunli Yang,Shuanghui Yin,Youjun Shang et al.
Shunli Yang et al.
Background: Immunomagnetic nanobead (IMNB) labeled with specific antibody, has been demonstrated to be useful for the capturing and detection of viruses. ...
The codon-optimized Δ(6)-desaturase gene of Pythium sp. as an empowering tool for engineering n3/n6 polyunsaturated fatty acid biosynthesis [0.03%]
来自Pythium sp.的优化密码子的Δ(6)去饱和酶基因，作为一种工程化n3 / n6多不饱和脂肪酸生物合成的工具
Sukanya Jeennor,Pattsarun Cheawchanlertfa,Sarinya Suttiwattanakul et al.
Sukanya Jeennor et al.
Background: The ∆(6)-desaturase gene, encoding a key enzyme in the biosynthesis of polyunsaturated fatty acids, has potential in pharmaceutical and nutraceutical applications. ...
Using proteomic analysis to investigate uniconazole-induced phytohormone variation and starch accumulation in duckweed (Landoltia punctata) [0.03%]
利用蛋白质组学分析苦草（Landoltia punctata）中高效霉菌素诱导的植物激素变化及淀粉累积机制
Mengjun Huang,Yang Fang,Yang Liu et al.
Mengjun Huang et al.
Background: Duckweed (Landoltia punctata) has the potential to remediate wastewater and accumulate enormous amounts of starch for bioethanol production. Using systematical screening, we determined that the highest biomass...
Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations [0.03%]
基于Cowpea氯绿斑驳病毒样颗粒的表位展示骨架的可行性研究
Afshin Hassani-Mehraban,Sjoerd Creutzburg,Luc van Heereveld et al.
Afshin Hassani-Mehraban et al.
Background & methods: Within the last decade Virus-Like Particles (VLPs) have increasingly received attention from scientists for their use as a carrier of (peptide) molecules or as scaffold to present epitopes for use in...
Immobilization of trypsin in organic and aqueous media for enzymatic peptide synthesis and hydrolysis reactions [0.03%]
有机和水相介质中固定胰蛋白酶并用于肽合成及酶解反应的研究
Julia Stolarow,Manuel Heinzelmann,Wladimir Yeremchuk et al.
Julia Stolarow et al.
Background: Immobilization of enzymes onto different carriers increases enzyme's stability and reusability within biotechnological and pharmaceutical applications. However, some immobilization techniques are associated wi...
Comparative Study
BMC biotechnology. 2015 Aug 19:15:77. DOI:10.1186/s12896-015-0196-y 2015