Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus [0.03%]
来自Pyrococcus furiosus的可溶性氢酶的产生及应用
Chang-Hao Wu,Patrick M McTernan,Mary E Walter et al.
Chang-Hao Wu et al.
Hydrogen gas is a potential renewable alternative energy carrier that could be used in the future to help supplement humanity's growing energy needs. Unfortunately, current industrial methods for hydrogen production are expensive or environ...
Frédéric Pecorari,Vickery L Arcus,Juergen Wiegel
Frédéric Pecorari
A Novel Highly Thermostable Multifunctional Beta-Glycosidase from Crenarchaeon Acidilobus saccharovorans [0.03%]
一株来自高温古菌Acidilobus saccharovorans的多功能β-糖苷酶基因的克隆、表达及初步应用研究
Vadim M Gumerov,Andrey L Rakitin,Andrey V Mardanov et al.
Vadim M Gumerov et al.
We expressed a putative β-galactosidase Asac_1390 from hyperthermophilic crenarchaeon Acidilobus saccharovorans in Escherichia coli and purified the recombinant enzyme. Asac_1390 is composed of 490 amino acid residues and showed high seque...
Archaeal MCM Proteins as an Analog for the Eukaryotic Mcm2-7 Helicase to Reveal Essential Features of Structure and Function [0.03%]
模式古菌MCM蛋白的结构与功能研究揭示了真核生物Mcm2-7解旋酶的基本特征
Justin M Miller,Eric J Enemark
Justin M Miller
In eukaryotes, the replicative helicase is the large multisubunit CMG complex consisting of the Mcm2-7 hexameric ring, Cdc45, and the tetrameric GINS complex. The Mcm2-7 ring assembles from six different, related proteins and forms the core...
From Structure-Function Analyses to Protein Engineering for Practical Applications of DNA Ligase [0.03%]
从结构功能分析到蛋白质工程：DNA连接酶的实际应用
Maiko Tanabe,Yoshizumi Ishino,Hirokazu Nishida
Maiko Tanabe
DNA ligases are indispensable in all living cells and ubiquitous in all organs. DNA ligases are broadly utilized in molecular biology research fields, such as genetic engineering and DNA sequencing technologies. Here we review the utilizati...
Untapped Resources: Biotechnological Potential of Peptides and Secondary Metabolites in Archaea [0.03%]
未被开发的资源：古菌中肽和次生代谢物的生物技术潜力
James C Charlesworth,Brendan P Burns
James C Charlesworth
Archaea are an understudied domain of life often found in "extreme" environments in terms of temperature, salinity, and a range of other factors. Archaeal proteins, such as a wide range of enzymes, have adapted to function under these extre...
Cecilia R Chambers,Wayne M Patrick
Cecilia R Chambers
With their ability to catalyse the formation of phosphodiester linkages, DNA ligases and RNA ligases are essential tools for many protocols in molecular biology and biotechnology. Currently, the nucleic acid ligases from bacteriophage T4 ar...
Jennifer A Littlechild
Jennifer A Littlechild
Archaeal enzymes are playing an important role in industrial biotechnology. Many representatives of organisms living in "extreme" conditions, the so-called Extremophiles, belong to the archaeal kingdom of life. This paper will review studie...
Biochemical Characterisation of Phage Pseudomurein Endoisopeptidases PeiW and PeiP Using Synthetic Peptides [0.03%]
基于合成肽研究噬菌体假肽聚糖内小肽酶PeiP和PeiW的生化性质
Linley R Schofield,Amy K Beattie,Catherine M Tootill et al.
Linley R Schofield et al.
Pseudomurein endoisopeptidases cause lysis of the cell walls of methanogens by cleaving the isopeptide bond Ala-ε-Lys in the peptide chain of pseudomurein. PeiW and PeiP are two thermostable pseudomurein endoisopeptidases encoded by phage ...
Ecology and Distribution of Thaumarchaea in the Deep Hypolimnion of Lake Maggiore [0.03%]
Lake Maggiore深水层产甲烷古菌的生态与分布
Manuela Coci,Nina Odermatt,Michaela M Salcher et al.
Manuela Coci et al.
Ammonia-oxidizing Archaea (AOA) play an important role in the oxidation of ammonia in terrestrial, marine, and geothermal habitats, as confirmed by a number of studies specifically focused on those environments. Much less is known about the...